Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,796	A→G	Q172Q (CAA→CAG)	ampE →	protein AmpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,796	0	A	G	100.0%	247.9 / NA	66	Q172Q (CAA→CAG)	ampE	protein AmpE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/42);  total (24/42)

TTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGATCGGT  >  NC_000913/119665‑119916                                                                                                                                    |                                                                                                                         ttGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTActggct                                                                                                                  <  1:595242/140‑1 (MQ=255)   gCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTActggctgg                                                                                                                >  2:1082075/1‑140 (MQ=255)   gCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTActggctgg                                                                                                                >  2:154802/1‑140 (MQ=255)     gTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGgcg                                                                                                              <  2:425305/140‑1 (MQ=255)     gTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGgcg                                                                                                              <  2:213111/140‑1 (MQ=255)     gTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGgcg                                                                                                              <  2:249479/140‑1 (MQ=255)       gAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGcgcg                                                                                                            <  1:893332/140‑1 (MQ=255)        aGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGTGCGa                                                                                                           >  1:62104/1‑140 (MQ=255)                    cATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGAcgccg                                                                                               <  1:631314/140‑1 (MQ=255)                    cATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGAcgccg                                                                                               >  2:468965/1‑140 (MQ=255)                      ttGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGca                                                                                             <  2:884456/140‑1 (MQ=255)                      ttGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGca                                                                                             <  1:558181/140‑1 (MQ=255)                       tGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGcat                                                                                            >  1:170662/1‑140 (MQ=255)                       tGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGcat                                                                                            <  2:19355/140‑1 (MQ=255)                       tGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGcat                                                                                            <  2:925103/140‑1 (MQ=255)                              gATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGtt                                                                                     <  1:154802/140‑1 (MQ=255)                                        cGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCgg                                                                           >  2:282189/1‑140 (MQ=255)                                             ttATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTg                                                                      >  2:567312/1‑140 (MQ=255)                                               aTCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGCTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGAt                                                                    <  2:929531/140‑1 (MQ=255)                                                tCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATg                                                                   <  1:468965/140‑1 (MQ=255)                                                tCTTGCACCGCTGTTCTCGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGTGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATg                                                                   <  2:506477/140‑1 (MQ=255)                                                 cTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGc                                                                  <  2:665072/140‑1 (MQ=255)                                                  ttGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGcc                                                                 <  1:1067990/140‑1 (MQ=255)                                                    gCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGTGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGt                                                               <  2:62104/140‑1 (MQ=255)                                                    gCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGt                                                               <  1:282189/140‑1 (MQ=255)                                                    gCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGt                                                               <  2:170662/140‑1 (MQ=255)                                                     cACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTg                                                              >  2:918859/1‑140 (MQ=255)                                                         gCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTc                                                          <  2:357700/140‑1 (MQ=255)                                                         gCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTc                                                          <  1:656644/140‑1 (MQ=255)                                                         gCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTc                                                          <  1:284608/140‑1 (MQ=255)                                                         gCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTc                                                          <  1:1082075/140‑1 (MQ=255)                                                         gCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTc                                                          <  1:918859/140‑1 (MQ=255)                                                          cTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCa                                                         >  1:361150/1‑140 (MQ=255)                                                             ttCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTg                                                                      >  2:1016529/1‑124 (MQ=255)                                                             ttCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTg                                                                      <  1:1016529/124‑1 (MQ=255)                                                             ttCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGt                                                      <  1:567312/140‑1 (MQ=255)                                                                                gAACCTGGGGACCCGTTGCGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGtt                                   <  1:102510/140‑1 (MQ=255)                                                                                gAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGt                                    <  1:352206/139‑1 (MQ=255)                                                                                gAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGt                                    >  2:352206/1‑139 (MQ=255)                                                                                  aCCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCg                                 >  2:1138022/1‑140 (MQ=255)                                                                                  aCCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCg                                 >  1:872541/1‑140 (MQ=255)                                                                                   ccTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGt                                >  1:446230/1‑140 (MQ=255)                                                                                      ggggACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCtt                             <  2:446230/140‑1 (MQ=255)                                                                                        ggACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGc                           >  2:590979/1‑140 (MQ=255)                                                                                        ggACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGc                           <  1:590979/140‑1 (MQ=255)                                                                                        ggACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGc                           <  2:740607/140‑1 (MQ=255)                                                                                           cccGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCggg                        >  1:797891/1‑140 (MQ=255)                                                                                           cccGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCggg                        >  1:213953/1‑140 (MQ=255)                                                                                            ccGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTg                                         <  2:442307/122‑1 (MQ=255)                                                                                            ccGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTg                                         >  1:442307/1‑122 (MQ=255)                                                                                            ccGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCgggg                       >  2:164604/1‑139 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  1:720436/140‑1 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  1:766107/140‑1 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  1:164604/140‑1 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      >  1:80261/1‑140 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  2:544855/140‑1 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  1:1138022/140‑1 (MQ=255)                                                                                             cGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGACCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtg                      <  1:192534/140‑1 (MQ=255)                                                                                              gTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGc                           <  1:457383/134‑1 (MQ=255)                                                                                              gTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGc                           >  2:457383/1‑134 (MQ=255)                                                                                              gTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGgtgt                     <  2:139004/140‑1 (MQ=255)                                                                                                  cGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGta                 <  2:361150/140‑1 (MQ=255)                                                                                                         ggggTATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCt          >  2:1112285/1‑140 (MQ=255)                                                                                                             tATGCGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGAt      <  2:231501/140‑1 (MQ=255)                                                                                                                 cGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGATCGGt  <  2:80261/140‑1 (MQ=255)                                                                                                                 cGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGATCGGt  >  2:210514/1‑140 (MQ=255)                                                                                                                 cGTTTTTGCGTGCATGGCAGTACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGATCGGt  >  1:1129837/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                         TTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGGGAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCATCATCGTTTACAGTCCGGCATTGATGCCGTGCTTCATGTACTGGATTGGGTGCCGGTTCGTCTTGCGGGTGTGGTATATGCCTTGATCGGT  >  NC_000913/119665‑119916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑