Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,230	T→C	D308G (GAC→GGC)	hprS ←	sensory histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,230	0	T	C	100.0%	222.6 / NA	59	D308G (GAC→GGC)	hprS	sensory histidine kinase HprS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

ACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGAC  >  NC_000913/2037096‑2037365                                                                                                                                       |                                                                                                                                        aCATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTTGACTTCCTTATTGAGAGAAAGCGAGCCCAGt                                                                                                                                    <  1:430668/140‑1 (MQ=255)   aTTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGttt                                                                                                                                  <  2:427645/140‑1 (MQ=255)    ttCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTc                                                                                                                                 >  2:841032/1‑140 (MQ=255)    ttCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTc                                                                                                                                 >  2:453316/1‑140 (MQ=255)     tCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCa                                                                                                                                >  1:15466/1‑140 (MQ=255)            agtaAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAAc                                                                                                                         >  1:967612/1‑140 (MQ=255)             gtaAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACa                                                                                                                        <  1:453316/140‑1 (MQ=255)             gtaAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACa                                                                                                                        <  1:150543/140‑1 (MQ=255)             gtaAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACa                                                                                                                        >  2:893556/1‑140 (MQ=255)                aaaTTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTg                                                                                                                     >  1:961384/1‑140 (MQ=255)                        tCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATc                                                                                                             >  1:63896/1‑140 (MQ=255)                             aaaGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGccc                                                                                                        <  1:571838/140‑1 (MQ=255)                                 aTTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGc                                                                                                    <  1:38889/140‑1 (MQ=255)                                    tGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAg                                                                                                 <  2:967612/140‑1 (MQ=255)                                      cTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGaa                                                                                               <  1:841032/140‑1 (MQ=255)                                      cTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGaa                                                                                               <  1:947019/140‑1 (MQ=255)                                      cTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGaa                                                                                               <  2:216778/140‑1 (MQ=255)                                      cTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGaa                                                                                               <  1:1007169/140‑1 (MQ=255)                                          ttGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAg                                                                                           <  1:1083379/140‑1 (MQ=255)                                           tGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCa                                                                                                   >  2:249280/1‑131 (MQ=255)                                           tGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCa                                                                                                   <  1:249280/131‑1 (MQ=255)                                           tGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                                          >  1:773961/1‑140 (MQ=255)                                                tCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCaa                                                                                                  >  1:990218/1‑127 (MQ=255)                                                tCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCaa                                                                                                  <  2:990218/127‑1 (MQ=255)                                                tCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGtt                                                                                     >  2:917502/1‑140 (MQ=255)                                                  gACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTtct                                                                                   <  2:15466/140‑1 (MQ=255)                                                              aaTCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACcgcg                                                                       >  1:594448/1‑140 (MQ=255)                                                              aaTCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACcgcg                                                                       <  1:917502/140‑1 (MQ=255)                                                              aaTCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACcgcg                                                                       <  2:1089236/140‑1 (MQ=255)                                                               aTCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGa                                                                      >  1:955012/1‑140 (MQ=255)                                                                             tGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGct                                                        <  2:961384/140‑1 (MQ=255)                                                                              gAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAata                                                                  <  1:358517/129‑1 (MQ=255)                                                                              gAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAata                                                                  >  2:358517/1‑129 (MQ=255)                                                                                  ggTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCa                                                   <  1:364193/140‑1 (MQ=255)                                                                                    tATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAAt                                                 >  2:218326/1‑140 (MQ=255)                                                                                      ttCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGt                                               >  1:51732/1‑140 (MQ=255)                                                                                              aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCa                                       >  2:625240/1‑140 (MQ=255)                                                                                              aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCa                                       >  2:137877/1‑140 (MQ=255)                                                                                              aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCa                                       >  2:212224/1‑140 (MQ=255)                                                                                              aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCa                                       >  1:588455/1‑140 (MQ=255)                                                                                               gTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCaa                                      <  1:870687/140‑1 (MQ=255)                                                                                                tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAAt                                     <  1:137877/140‑1 (MQ=255)                                                                                                tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAAt                                     >  1:149626/1‑140 (MQ=255)                                                                                                tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAAt                                     >  1:1046075/1‑140 (MQ=255)                                                                                                tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAAt                                     >  2:118454/1‑140 (MQ=255)                                                                                                      aaaTTTTCGACTTCCTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGtttt                               <  1:893556/140‑1 (MQ=255)                                                                                                                    cTTATTGAGAGAAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGa                 <  2:63896/140‑1 (MQ=255)                                                                                                                          gagagaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTc           <  1:218326/140‑1 (MQ=255)                                                                                                                            gagaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTg         <  1:625240/140‑1 (MQ=255)                                                                                                                             agaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg        >  1:323197/1‑140 (MQ=255)                                                                                                                             agaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg        >  2:465841/1‑140 (MQ=255)                                                                                                                             agaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg        >  1:48787/1‑140 (MQ=255)                                                                                                                              gaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACt             <  1:1040589/134‑1 (MQ=255)                                                                                                                              gaAAGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACt             >  2:1040589/1‑134 (MQ=255)                                                                                                                               aaaGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGtt      >  2:792304/1‑140 (MQ=255)                                                                                                                                 aGCGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTg    >  2:655233/1‑140 (MQ=255)                                                                                                                                   cGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGAc  <  1:118454/140‑1 (MQ=255)                                                                                                                                   cGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGAc  <  2:773961/140‑1 (MQ=255)                                                                                                                                   cGAGCCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGAc  <  2:149626/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGAC  >  NC_000913/2037096‑2037365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑