Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,598	T→C	R433R (CGT→CGC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,598	0	T	C	100.0%	191.9 / NA	52	R433R (CGT→CGC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/25);  total (27/25)

GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277461‑2277734                                                                                                                                          |                                                                                                                                         ggCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCAt                                                                                                                                        >  2:102432/1‑140 (MQ=255)     tACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCg                                                                                                                                    <  1:919401/140‑1 (MQ=255)     tACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCg                                                                                                                                    <  1:867388/140‑1 (MQ=255)      aCGGGTTCACAAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGa                                                                                                                                   >  2:935769/1‑140 (MQ=255)          gTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGc                                                                                                                               <  1:24619/140‑1 (MQ=255)           ttCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGcc                                                                                                                              >  2:461420/1‑140 (MQ=255)                           ttCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTa                                                                                                              <  2:345049/140‑1 (MQ=255)                               gCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                          <  1:883183/140‑1 (MQ=255)                               gCCGCACAACGCGAACAACAAGAGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                          <  2:30296/140‑1 (MQ=255)                                   cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCttg                                                                                                      <  1:102432/140‑3 (MQ=255)                                   cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                      >  2:278950/1‑140 (MQ=255)                                    acaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGc                                                                                                     >  1:931499/1‑140 (MQ=255)                                        cgcgAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGAt                                                                                                 <  1:702840/140‑1 (MQ=255)                                                acaaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtg                                                                                         <  2:282602/140‑1 (MQ=255)                                                acaaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtg                                                                                         >  1:256689/1‑140 (MQ=255)                                                 caaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtga                                                                                        <  2:256689/140‑1 (MQ=255)                                                       aTAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAAcc                                                                                  >  2:410314/1‑140 (MQ=255)                                                         aGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGa                                                                                >  1:1027557/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  2:830164/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  1:353538/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  1:205443/1‑140 (MQ=255)                                                                     tGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCg                                                                    >  1:289440/1‑140 (MQ=255)                                                                     tGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCg                                                                    >  1:1037466/1‑140 (MQ=255)                                                                     tGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCg                                                                    >  1:278790/1‑140 (MQ=255)                                                                      gAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGa                                                                   <  1:612309/140‑1 (MQ=255)                                                                      gAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGa                                                                   <  1:851235/140‑1 (MQ=255)                                                                      gAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGa                                                                   <  1:278950/140‑1 (MQ=255)                                                                        aGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACa                                                                 >  1:359812/1‑140 (MQ=255)                                                                            ggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAAc                                                             >  2:831129/1‑140 (MQ=255)                                                                                  gATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                       <  2:776995/140‑1 (MQ=255)                                                                                    tCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAgg                                                     >  2:650883/1‑140 (MQ=255)                                                                                    tCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAgg                                                     >  2:950965/1‑140 (MQ=255)                                                                                      cTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcg                                                   >  1:124061/1‑140 (MQ=255)                                                                                       tGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGgcgc                                                  <  2:124061/140‑1 (MQ=255)                                                                                             acGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAatat                                            >  1:741500/1‑140 (MQ=255)                                                                                                ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                         <  1:210938/140‑1 (MQ=255)                                                                                                 cACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGAc                                        >  1:187355/1‑140 (MQ=255)                                                                                                  aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:461420/140‑1 (MQ=255)                                                                                                         tCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:40807/133‑1 (MQ=255)                                                                                                         tCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       >  2:40807/1‑133 (MQ=255)                                                                                                                             gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  1:1020012/1‑140 (MQ=255)                                                                                                                             gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  2:360258/1‑140 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         >  2:1087209/1‑140 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         <  2:1037466/140‑1 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         <  2:1027557/140‑1 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         >  1:234263/1‑140 (MQ=255)                                                                                                                                aGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACAGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCaa         >  1:694804/1‑140 (MQ=255)                                                                                                                                 gCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAAc        <  1:360258/140‑1 (MQ=255)                                                                                                                                   gACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACAGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTg      <  2:694804/140‑1 (MQ=255)                                                                                                                                     cAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGc    <  2:278790/140‑1 (MQ=255)                                                                                                                                       aCGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCt  <  2:359812/140‑1 (MQ=255)                                                                                                                                       aCGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCt  <  2:234263/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277461‑2277734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑