Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,298	T→C	A112A (GCA→GCG)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,298	0	T	C	100.0%	125.0 / NA	36	A112A (GCA→GCG)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

GGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGC  >  NC_000913/2363173‑2363431                                                                                                                              |                                                                                                                                      ggTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTcc                                                                                                                         <  1:28297/140‑1 (MQ=255)  gTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCa                                                                                                                        >  1:825959/1‑140 (MQ=255)         gggAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGt                                                                                                                 >  1:1107499/1‑140 (MQ=255)         gggAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGt                                                                                                                 <  1:480291/140‑1 (MQ=255)            aGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGa                                                                                                              >  2:705083/1‑140 (MQ=255)               aaaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAAt                                                                                                           >  1:496287/1‑140 (MQ=255)                aaGAACATCAGGCAACGATTAAGCTGCGCGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATa                                                                                                          >  2:47878/1‑140 (MQ=255)                aaGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATa                                                                                                          >  1:978174/1‑140 (MQ=255)                 aGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAg                                                                                                         <  2:954418/140‑1 (MQ=255)                 aGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAg                                                                                                         >  2:273384/1‑140 (MQ=255)                   aaCATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCg                                                                                                       <  2:496287/140‑1 (MQ=255)                   aaCATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCg                                                                                                       <  1:1064469/140‑1 (MQ=255)                      aTCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTc                                                                                                    >  1:1145179/1‑140 (MQ=255)                       tCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTcc                                                                                                   >  1:1009077/1‑140 (MQ=255)                                  ttAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAg                                                                                        <  1:299484/140‑1 (MQ=255)                                    aaGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGcc                                                                                      <  2:1009077/140‑1 (MQ=255)                                      gCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAg                                                                                    <  1:273384/140‑1 (MQ=255)                                         gCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCt                                                                                 <  1:779329/140‑1 (MQ=255)                                            cGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCa                                                                              >  1:620345/1‑140 (MQ=255)                                               cAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATgc                                                                           >  2:466105/1‑140 (MQ=255)                                                      acaGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCa                                                                    <  1:466105/140‑1 (MQ=255)                                                             gTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTc                                                             >  1:1082267/1‑140 (MQ=255)                                                                        gTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTg                                                  >  1:582199/1‑140 (MQ=255)                                                                                       aGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGa                                      >  2:974690/1‑137 (MQ=255)                                                                                       aGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGa                                      <  1:974690/137‑1 (MQ=255)                                                                                       aGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTg                                   >  1:763424/1‑140 (MQ=255)                                                                                         cACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTgcg                                 >  1:731774/1‑140 (MQ=255)                                                                                           cTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCt                               <  1:705083/140‑1 (MQ=255)                                                                                              gcaggcagCTCCGCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTaa                            >  1:587034/1‑140 (MQ=255)                                                                                                          gCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt                <  2:1145179/140‑1 (MQ=255)                                                                                                          gCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt                <  2:825959/140‑1 (MQ=255)                                                                                                          gCTTGTTTACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt                <  2:978174/140‑1 (MQ=255)                                                                                                                ttACGGTTGCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTcc          >  2:676058/1‑140 (MQ=255)                                                                                                                        gCTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCccgc  >  1:704140/1‑140 (MQ=255)                                                                                                                         cTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt                >  2:328891/1‑125 (MQ=255)                                                                                                                         cTCGGCGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTggt                <  1:328891/125‑1 (MQ=255)                                                                                                                              |                                                                                                                                      GGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACGTTCGTGGAAATAGCGTTCCATCTCTTTGAGCCAGGCTTCATGCAGCACCAGGCCTTCACCTTTTGCTGTCGCAGCGGCGAGTGCGCTTAAATCATCGCTGGTCGGTCCCAGCCCGC  >  NC_000913/2363173‑2363431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑