Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,406,122	A→G	Y174H (TAC→CAC)	lrhA ←	DNA‑binding transcriptional dual regulator LrhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,406,122	0	A	G	100.0%	243.1 / NA	67	Y174H (TAC→CAC)	lrhA	DNA‑binding transcriptional dual regulator LrhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/28);  total (39/28)

GCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGC  >  NC_000913/2405986‑2406255                                                                                                                                         |                                                                                                                                      gCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCa                                                                                                                                    <  1:119312/140‑1 (MQ=255) gCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCa                                                                                                                                    <  1:896337/140‑1 (MQ=255)         aGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGTGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTg                                                                                                                            <  2:101880/140‑1 (MQ=255)            cAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCg                                                                                                                         <  2:915842/140‑1 (MQ=255)            cAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCg                                                                                                                         <  1:208514/140‑1 (MQ=255)               tCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAgg                                                                                                                      >  2:340771/1‑140 (MQ=255)               tCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAgg                                                                                                                      >  1:780182/1‑140 (MQ=255)               tCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAgg                                                                                                                      >  1:734094/1‑140 (MQ=255)                 gCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTa                                                                                                                    <  2:80488/140‑1 (MQ=255)                    aTGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGc                                                                                                                 >  1:157479/1‑140 (MQ=255)                    aTGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGc                                                                                                                 <  2:704673/140‑1 (MQ=255)                     tGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCa                                                                                                                >  1:651797/1‑140 (MQ=255)                             cTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAAt                                                                                                        <  2:74717/140‑1 (MQ=255)                                cTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGGGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAg                                                                                                                       <  1:811075/122‑1 (MQ=255)                                cTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAg                                                                                                                       >  2:811075/1‑122 (MQ=255)                                 tttATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCt                                                                                                    >  1:450075/1‑140 (MQ=255)                                 tttATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCt                                                                                                    >  1:202389/1‑140 (MQ=255)                                  ttattaAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCtt                                                                                                   >  1:57142/1‑140 (MQ=255)                                          cGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTg                                                                                           >  1:257440/1‑140 (MQ=255)                                          cGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTg                                                                                           >  1:190030/1‑140 (MQ=255)                                             cGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAg                                                                                        >  2:559553/1‑140 (MQ=255)                                                 aGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACg                                                                                    <  1:340771/140‑1 (MQ=255)                                                   cACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGAt                                                                                  <  2:734094/140‑1 (MQ=255)                                                     ccATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATgg                                                                                <  2:157479/140‑1 (MQ=255)                                                     ccATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATgg                                                                                <  2:780182/140‑1 (MQ=255)                                                            gcgAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTa                                                                         <  2:190030/140‑1 (MQ=255)                                                             cgAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTAc                                                                        >  2:208164/1‑140 (MQ=255)                                                               aaaCGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCa                                                                      >  2:852360/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  1:660381/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  1:1142416/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  2:759878/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  2:361891/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  2:752620/1‑140 (MQ=255)                                                                               tcCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTg                                                      >  1:140140/1‑140 (MQ=255)                                                                                       tACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGCGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCa                                              >  2:506464/1‑140 (MQ=255)                                                                                         cAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGc                                            >  2:592993/1‑140 (MQ=255)                                                                                            gagGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCAtt                                         >  2:1035210/1‑140 (MQ=255)                                                                                             agGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCAttt                                        <  1:559553/140‑1 (MQ=255)                                                                                               ggATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCt                                      <  2:450075/140‑1 (MQ=255)                                                                                               ggATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCt                                      <  2:104163/140‑1 (MQ=255)                                                                                                gATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTg                                     <  1:361891/140‑1 (MQ=255)                                                                                                 aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTg                                     <  1:929974/139‑1 (MQ=255)                                                                                                 aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTg                                     >  2:929974/1‑139 (MQ=255)                                                                                                 aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGc                                    <  2:602318/140‑1 (MQ=255)                                                                                                 aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGc                                    >  1:11192/1‑140 (MQ=255)                                                                                                 aTCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGc                                    >  1:602318/1‑140 (MQ=255)                                                                                                    ggCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCat                                 >  1:202415/1‑140 (MQ=255)                                                                                                      cTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCatat                               <  1:752620/140‑1 (MQ=255)                                                                                                       tCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGTGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCAtata                              >  2:867023/1‑140 (MQ=255)                                                                                                        cGCCTTTTTGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGGAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAg                             >  2:723469/1‑140 (MQ=255)                                                                                                         gCCTTTTTGCAATTTGTATTCCGCTGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAgg                            >  2:263470/1‑140 (MQ=255)                                                                                                          ccTTTTTGCAATATGTATTCCGCTGCGCAGCGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGc                           <  1:506464/140‑1 (MQ=255)                                                                                                             ttttGCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCAtt                        <  1:172591/140‑1 (MQ=255)                                                                                                                 gCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGt                    <  2:942723/140‑1 (MQ=255)                                                                                                                 gCAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGt                    <  1:852360/140‑1 (MQ=255)                                                                                                                  cAATATGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGtt                   <  2:257440/140‑1 (MQ=255)                                                                                                                       tGTATTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAAcg              <  1:759878/140‑1 (MQ=255)                                                                                                                          aTTCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCgg           >  1:104964/1‑140 (MQ=255)                                                                                                                            tCCGCTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGAc         >  1:743645/1‑140 (MQ=255)                                                                                                                                cTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATcc     >  1:19018/1‑140 (MQ=255)                                                                                                                                cTGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATcc     >  1:94494/1‑140 (MQ=255)                                                                                                                                 tGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATcc     <  1:654354/139‑1 (MQ=255)                                                                                                                                 tGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATcc     >  2:654354/1‑139 (MQ=255)                                                                                                                                 tGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCa    >  1:553425/1‑140 (MQ=255)                                                                                                                                 tGCGCAGTGCCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCa    >  2:1004399/1‑140 (MQ=255)                                                                                                                                 tGCGCAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCa    >  1:456518/1‑140 (MQ=255)                                                                                                                                   cgcAGTGCCAGTGTGCTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAgc  >  1:549765/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GCGACATAAGCCAGTCGCCATGGAATATCTGCTTTATTAAGCGTCGCCAGCACCATATCGCGAAACGGGCTAGGATCATCCAGCAATACAAGAGGGATCGGCTCGCCTTTTTGCAATATGTATTCCGCTGCGCAGTACCAGTGTGTTGGCGAGGTACGCAGGTTCAATGCCTTAAACGCTGAAGGACGATGGGTGGTTACCATCAAATCCACTTCCTGGGATTCCAGCATTTCTGCCATATAGGCATTACGTTTAACGCGGACATCCAGC  >  NC_000913/2405986‑2406255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑