Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,099	G→C	A85P (GCC→CCC)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,099	0	G	C	100.0%	255.9 / NA	69	A85P (GCC→CCC)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (33/36);  total (33/36)

TCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGC  >  NC_000913/2477962‑2478232                                                                                                                                          |                                                                                                                                      tcGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGccc                                                                                                                                     >  2:1097382/1‑140 (MQ=255) tcGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGccc                                                                                                                                     >  2:442579/1‑140 (MQ=255)     gggAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCag                                                                                                                                 >  2:296343/1‑140 (MQ=255)      ggAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCaga                                                                                                                                >  2:558943/1‑140 (MQ=255)          cGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCagaaaga                                                                                                                            >  2:1047275/1‑140 (MQ=255)                 gggCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTc                                                                                                                     <  1:296343/140‑1 (MQ=255)                  ggCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCt                                                                                                                    <  2:165337/140‑1 (MQ=255)                    cATGGGGCCACTGGATATGGTAAATGCTATCGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGa                                                                                                                  <  2:705869/140‑1 (MQ=255)                     aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCagaaagaa                                                                                                                           <  1:653437/130‑1 (MQ=255)                     aTGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCagaaagaa                                                                                                                           >  2:653437/1‑130 (MQ=255)                       ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGacac                                                                                                               >  2:813602/1‑140 (MQ=255)                       ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGacac                                                                                                               >  1:4117/1‑140 (MQ=255)                       ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGacac                                                                                                               >  2:744276/1‑140 (MQ=255)                       ggggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGacac                                                                                                               >  2:684717/1‑140 (MQ=255)                         ggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTTGTCTGACACtt                                                                                                             >  2:228600/1‑140 (MQ=255)                         ggCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACGCtt                                                                                                             >  1:539502/1‑140 (MQ=255)                                   tatGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGcc                                                                                                   >  2:1077985/1‑140 (MQ=255)                                    atGGTAAATGCTATTGAAAGTGGAATTGGTGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCg                                                                                                  >  1:259141/1‑140 (MQ=255)                                      ggTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                                >  1:799096/1‑140 (MQ=255)                                          aaTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACg                                                                                                        >  1:377647/1‑128 (MQ=255)                                          aaTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACg                                                                                                        <  2:377647/128‑1 (MQ=255)                                          aaTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                            <  1:367354/140‑1 (MQ=255)                                          aaTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCt                                                                                            <  1:126575/140‑1 (MQ=255)                                           aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCtt                                                                                           >  2:887509/1‑140 (MQ=255)                                           aTGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCtt                                                                                           >  1:763962/1‑140 (MQ=255)                                            tGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCttt                                                                                          >  1:746610/1‑140 (MQ=255)                                            tGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCttt                                                                                          >  1:755308/1‑140 (MQ=255)                                                aTTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                                      <  1:476770/140‑1 (MQ=255)                                                aTTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGt                                                                                      <  2:799096/140‑1 (MQ=255)                                                 ttGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTTGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGtt                                                                                     <  1:228600/140‑1 (MQ=255)                                                  tGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                                >  1:469801/1‑128 (MQ=255)                                                  tGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCt                                                                                                <  2:469801/128‑1 (MQ=255)                                                   gAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACGCTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                                   <  2:539502/140‑1 (MQ=255)                                                   gAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                                   <  2:81086/140‑1 (MQ=255)                                                   gAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                                   <  2:1051859/140‑1 (MQ=255)                                                   gAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGa                                                                                   <  2:554231/140‑1 (MQ=255)                                                       gTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTc                                                                               >  2:1058305/1‑140 (MQ=255)                                                           aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                           >  1:85565/1‑140 (MQ=255)                                                           aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                           >  1:840835/1‑140 (MQ=255)                                                           aaTTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTa                                                                           <  1:1085311/140‑1 (MQ=255)                                                               ggCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGt                                                                       <  1:813602/140‑1 (MQ=255)                                                                  ggAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCt                                                                    <  2:4117/140‑1 (MQ=255)                                                                  ggAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCt                                                                    >  2:550640/1‑140 (MQ=255)                                                                    aaCGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTgg                                                                  <  2:191819/140‑1 (MQ=255)                                                                      cGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGtt                                                                <  1:744276/140‑1 (MQ=255)                                                                         ctgggTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGc                                                             <  1:968696/138‑1 (MQ=255)                                                                            ggTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTg                                                          <  2:840835/140‑1 (MQ=255)                                                                                 cTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtg                                                     >  2:666591/1‑140 (MQ=255)                                                                                   cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                                   <  2:259141/140‑1 (MQ=255)                                                                                   cGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTtgtg                                                   <  1:1058305/140‑1 (MQ=255)                                                                                           ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                           <  1:558943/140‑1 (MQ=255)                                                                                           ttATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                           <  2:755308/140‑1 (MQ=255)                                                                                             aTCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGTCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                         >  1:1021035/1‑140 (MQ=255)                                                                                             aTCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCt                                         <  1:518965/140‑1 (MQ=255)                                                                                                   cTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCtgtttgt                                   <  1:442579/140‑1 (MQ=255)                                                                                                        cACGATACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGTCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAg                              <  2:1021035/140‑1 (MQ=255)                                                                                                            aTACTGGGAAAAATGATGGAAGCATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTggg                          >  2:833350/1‑140 (MQ=255)                                                                                                             tACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                           >  2:567912/1‑122 (MQ=255)                                                                                                             tACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACg                                           <  1:567912/122‑1 (MQ=255)                                                                                                             tACTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGc                         >  2:399509/1‑140 (MQ=255)                                                                                                              aCTGGGAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg                        <  1:684717/140‑1 (MQ=255)                                                                                                                   gAAAAATGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTg                   <  2:295682/140‑1 (MQ=255)                                                                                                                       aaTGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg                        <  1:780809/131‑1 (MQ=255)                                                                                                                       aaTGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGcg                        >  2:780809/1‑131 (MQ=255)                                                                                                                       aaTGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTAt               >  2:788400/1‑140 (MQ=255)                                                                                                                       aaTGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTAt               >  2:377232/1‑140 (MQ=255)                                                                                                                       aaTGATGGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTAt               >  1:1044459/1‑140 (MQ=255)                                                                                                                          gatgGAAGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGa            <  2:763962/140‑1 (MQ=255)                                                                                                                               aaGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCct       <  1:1097382/140‑1 (MQ=255)                                                                                                                               aaGTATCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCct       <  2:352101/140‑1 (MQ=255)                                                                                                                                    tCCGGGCCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGGGTCGTGCTATTGATTCCTCTGGc  >  1:795057/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TCGTGGGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGCGGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATGGAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCAGTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTGGGCGTCGTGCTATTGATTCCTCTGGC  >  NC_000913/2477962‑2478232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑