Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,944	T→C	T118A (ACG→GCG)	alaC ←	glutamate‑‑pyruvate aminotransferase AlaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,944	0	T	C	100.0%	202.3 / NA	54	T118A (ACG→GCG)	alaC	glutamate‑‑pyruvate aminotransferase AlaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/21);  total (33/21)

CTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAA  >  NC_000913/2497810‑2498080                                                                                                                                       |                                                                                                                                         ctcCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCa                                                                                                                                     >  1:1142005/1‑140 (MQ=255)         cGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGa                                                                                                                             <  1:236786/140‑1 (MQ=255)         cGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGa                                                                                                                             <  2:424600/140‑1 (MQ=255)            tGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATgc                                                                                                                          >  1:49867/1‑140 (MQ=255)                   aTCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGc                                                                                                                   >  2:1051073/1‑140 (MQ=255)                   aTCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGc                                                                                                                   >  1:94846/1‑140 (MQ=255)                             tCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGa                                                                                                         <  2:613617/140‑1 (MQ=255)                             tCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGa                                                                                                         >  1:866564/1‑140 (MQ=255)                               caccaGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAAc                                                                                                       <  1:179159/140‑1 (MQ=255)                                    gCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATa                                                                                                  <  2:1142005/140‑1 (MQ=255)                                       gCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTg                                                                                               >  1:927276/1‑140 (MQ=255)                                       gCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTg                                                                                               >  1:750942/1‑140 (MQ=255)                                             aGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATg                                                                                         <  2:750942/140‑1 (MQ=255)                                             aGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATg                                                                                         <  2:927276/140‑1 (MQ=255)                                                       cgcCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGAtt                                                                               >  1:223061/1‑140 (MQ=255)                                                               cAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCg                                                                       >  2:505325/1‑140 (MQ=255)                                                                      cgcgCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCaa                                                                >  1:1131341/1‑140 (MQ=255)                                                                       gcgcCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAAc                                                               >  1:856750/1‑140 (MQ=255)                                                                         gcCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCaa                                                                >  2:487227/1‑137 (MQ=255)                                                                         gcCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCaa                                                                <  1:487227/137‑1 (MQ=255)                                                                         gcCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAAcgt                                                             <  1:467916/140‑1 (MQ=255)                                                                           cATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAAcgtcg                                                           <  2:94846/140‑1 (MQ=255)                                                                              aaaTATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAg                                                        <  1:505325/140‑1 (MQ=255)                                                                              aaaTATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAg                                                        <  2:234745/140‑1 (MQ=255)                                                                              aaaTATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAg                                                        <  2:223061/140‑1 (MQ=255)                                                                                atatGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCg                                                      <  2:49867/140‑1 (MQ=255)                                                                                  atGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGAt                                                    >  2:841677/1‑140 (MQ=255)                                                                                   tGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATc                                                   >  2:1054605/1‑140 (MQ=255)                                                                                     gATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCt                                                 >  2:81933/1‑140 (MQ=255)                                                                                       tCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGa                                               >  1:886785/1‑140 (MQ=255)                                                                                          ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAc                                            >  1:382843/1‑140 (MQ=255)                                                                                          ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAc                                            >  1:7488/1‑140 (MQ=255)                                                                                          ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAc                                            >  1:634539/1‑140 (MQ=255)                                                                                          ggTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAc                                            >  2:88270/1‑140 (MQ=255)                                                                                           gTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCTATTTCAACGTCGTAGCGATCCTGATAcc                                           >  1:487229/1‑140 (MQ=255)                                                                                           gTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATAcc                                           >  2:305823/1‑140 (MQ=255)                                                                                               cTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCg                                       >  1:792606/1‑140 (MQ=255)                                                                                                ttGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCgg                                      >  1:1000287/1‑140 (MQ=255)                                                                                                         gCACCAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGgcg                             <  2:634539/140‑1 (MQ=255)                                                                                                            ccAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGa                          <  1:81933/140‑1 (MQ=255)                                                                                                             cAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGAc                         >  1:631847/1‑140 (MQ=255)                                                                                                             cAGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGAc                         >  1:554444/1‑140 (MQ=255)                                                                                                              aGCACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACg                        <  1:1051073/140‑1 (MQ=255)                                                                                                                cACCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTa                      >  2:1123886/1‑140 (MQ=255)                                                                                                                 aCCGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCTATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTaa                     <  2:487229/140‑1 (MQ=255)                                                                                                                   cGTGTCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAAcc                   <  2:886785/140‑1 (MQ=255)                                                                                                                    gtgtCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACcg                  >  2:935612/1‑140 (MQ=255)                                                                                                                    gtgtCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACcg                  >  1:312913/1‑140 (MQ=255)                                                                                                                     tgtCACCATGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACcgc                 >  1:575742/1‑140 (MQ=255)                                                                                                                             tGATCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGcc         >  2:593733/1‑140 (MQ=255)                                                                                                                               aTCCAGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCcgc       >  2:529338/1‑140 (MQ=255)                                                                                                                                   aGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGa   <  2:7488/140‑1 (MQ=255)                                                                                                                                   aGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGa   <  2:382843/140‑1 (MQ=255)                                                                                                                                   aGCGCCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGa   >  1:598598/1‑140 (MQ=255)                                                                                                                                    gcgcCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGaa  <  2:1000287/140‑1 (MQ=255)                                                                                                                                    gcgcCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGaa  <  2:856750/140‑1 (MQ=255)                                                                                                                                    gcgcCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGaa  <  2:866564/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGCGGCACTGAGCGTACCTGCGCCCCGGCAATCACCGCGCCATAAATATGGATCGGGTAACTTGGATTCGGCACCAGCACCGTGTCACCATGATCCAGCGTCGCCAGCATCAGATGCGCCAGGCCCTCTTTCGAACCAATAGTGACGATGGCTTCTGATTCCGGGTCGATTTCAACGTCGTAGCGATCCTGATACCAGCGGGAAATGGCGCGACGTAACCGCGGAATGCCGCGTGAA  >  NC_000913/2497810‑2498080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑