Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,572,290	G→A	A54V (GCC→GTC)	eutM ←	putative structural protein, ethanolamine utilization microcompartment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,572,290	0	G	A	100.0%	262.7 / NA	75	A54V (GCC→GTC)	eutM	putative structural protein, ethanolamine utilization microcompartment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/33);  total (42/33)

TGATTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGT  >  NC_000913/2572154‑2572426                                                                                                                                         |                                                                                                                                         tGATTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACtt                                                                                                                                       >  2:829007/1‑140 (MQ=255)   aTTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTa                                                                                                                                     >  2:230046/1‑140 (MQ=255)   aTTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTa                                                                                                                                     <  2:808284/140‑1 (MQ=255)   aTTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTa                                                                                                                                     >  2:776719/1‑140 (MQ=255)   aTTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTa                                                                                                                                     >  2:756902/1‑140 (MQ=255)    ttACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTAc                                                                                                                                    <  1:546507/140‑1 (MQ=255)     tACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACa                                                                                                                                   >  1:939351/1‑140 (MQ=255)     tACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACa                                                                                                                                   >  1:1059761/1‑140 (MQ=255)      aCAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAg                                                                                                                                  <  1:482036/140‑1 (MQ=255)       cAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAgg                                                                                                                                 >  2:880480/1‑140 (MQ=255)        aGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGc                                                                                                                                <  2:821861/140‑1 (MQ=255)         ggTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCt                                                                                                                               >  1:185412/1‑140 (MQ=255)         ggTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCt                                                                                                                               >  1:1083865/1‑140 (MQ=255)         ggTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCt                                                                                                                               >  2:209643/1‑140 (MQ=255)         ggTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCt                                                                                                                               >  1:392834/1‑140 (MQ=255)             gctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCaa                                                                                                                           >  1:1115203/1‑140 (MQ=255)              ctgctATCGCCTTTCAGGCCGATCGGGAAGCCTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAAc                                                                                                                          >  2:78604/1‑140 (MQ=255)              ctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAAc                                                                                                                          >  2:278026/1‑140 (MQ=255)              ctgctATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAAc                                                                                                                          >  1:426400/1‑140 (MQ=255)                          ttCAGGCCGATCGGGAAGTCTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAAc                                                                                                              <  1:78604/140‑1 (MQ=255)                          ttCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAAc                                                                                                              <  2:27308/140‑1 (MQ=255)                            cAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCa                                                                                                            <  2:426400/140‑1 (MQ=255)                              ggCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATg                                                                                                          >  2:827044/1‑140 (MQ=255)                              ggCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATg                                                                                                          >  1:789293/1‑140 (MQ=255)                               gCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATgg                                                                                                         <  1:129062/140‑1 (MQ=255)                                 cGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCa                                                                                                       <  2:185412/140‑1 (MQ=255)                                    tCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTa                                                                                                    >  1:413042/1‑140 (MQ=255)                                           gACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgc                                                                                             <  1:880480/140‑1 (MQ=255)                                              ttcttcCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgccgc                                                                                          >  1:173030/1‑140 (MQ=255)                                                cttcCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgccgccg                                                                                        >  1:51890/1‑140 (MQ=255)                                                cttcCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGgccgccg                                                                                        <  1:843630/140‑1 (MQ=255)                                                ctgccAGGTCGTCGTGCGGACGCGGGAGAACCTGTGCCGATACCAGCTCGCCGATACGCCGTGCCGTGGCGGCCCATGAATCGGTCGCGACTTTACACGCGGCATCTTCCCCACGAACCATGTCAGTACACAGgccgccg                                                                                        <  2:889885/137‑1 (MQ=255)                                                   ccAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCa                                                                                     <  2:939351/140‑1 (MQ=255)                                                   ccAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCa                                                                                     <  2:1059761/140‑1 (MQ=255)                                                     aGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAAt                                                                                   <  1:776719/140‑1 (MQ=255)                                                          gCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCt                                                                              <  1:829007/140‑1 (MQ=255)                                                              tGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAac                                                                          <  2:966980/140‑1 (MQ=255)                                                                cGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAacac                                                                        >  2:374921/1‑140 (MQ=255)                                                                   aCGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACaccaa                                                                     <  1:278026/140‑1 (MQ=255)                                                                   aCGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACaccaa                                                                     <  2:1083865/140‑1 (MQ=255)                                                                                  tACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                      <  2:51890/140‑1 (MQ=255)                                                                                  tACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                      <  1:976269/140‑1 (MQ=255)                                                                                  tACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                      <  1:230046/140‑1 (MQ=255)                                                                                   aCAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACgcgcgc                                                     >  2:757400/1‑140 (MQ=255)                                                                                    cAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCa                                                    <  2:149889/140‑1 (MQ=255)                                                                                    cAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCa                                                    <  1:756902/140‑1 (MQ=255)                                                                                     aGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAg                                                   >  2:339975/1‑140 (MQ=255)                                                                                      gATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGc                                                  <  1:827044/140‑1 (MQ=255)                                                                                       aTACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCt                                                 >  2:106071/1‑140 (MQ=255)                                                                                        tACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCtt                                                >  1:240340/1‑140 (MQ=255)                                                                                         aCCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgc                                                         <  1:1024444/130‑1 (MQ=255)                                                                                         aCCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgc                                                         >  2:1024444/1‑130 (MQ=255)                                                                                         aCCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCttt                                               >  1:660751/1‑140 (MQ=255)                                                                                            aGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCAc                                            >  1:532923/1‑140 (MQ=255)                                                                                             gCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCAcc                                           >  2:278701/1‑140 (MQ=255)                                                                                              cTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCa                                          >  1:677042/1‑140 (MQ=255)                                                                                               tCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCAt                                         <  2:392834/140‑1 (MQ=255)                                                                                               tCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGCCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGTGCGCAGCTTTCACCAt                                         >  1:475254/1‑140 (MQ=255)                                                                                                 gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                      <  2:793251/125‑1 (MQ=255)                                                                                                 gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGAcgcgcg                                                      >  1:793251/1‑125 (MQ=255)                                                                                                 gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATcg                                       >  1:251022/1‑140 (MQ=255)                                                                                                 gCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATcg                                       >  2:390293/1‑140 (MQ=255)                                                                                                   cGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATcgcg                                     >  2:238202/1‑140 (MQ=255)                                                                                                            gtgCCGCTGCGGCCCCTGCATCGGTCGCGACTTTACAGGCTGCATCATCCCCCCCAACCATGGCAGTACCCAGCCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGcc                            <  2:240340/140‑1 (MQ=255)                                                                                                               ccGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAg                                 >  2:206655/1‑132 (MQ=255)                                                                                                               ccGCTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAg                                 <  1:206655/132‑1 (MQ=255)                                                                                                                  cTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATc                      >  1:210505/1‑140 (MQ=255)                                                                                                                  cTGCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTCAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATc                      >  1:106250/1‑140 (MQ=255)                                                                                                                    gCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAg                    >  2:1120301/1‑140 (MQ=255)                                                                                                                    gCGGCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAg                    <  1:390293/140‑1 (MQ=255)                                                                                                                       gCACCTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGc                 >  1:526976/1‑140 (MQ=255)                                                                                                                          ccTGCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAAc              <  2:660751/140‑1 (MQ=255)                                                                                                                             gCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCaa                        >  1:979040/1‑127 (MQ=255)                                                                                                                             gCATCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCaa                        <  2:979040/127‑1 (MQ=255)                                                                                                                               aTCGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGc         >  1:160385/1‑140 (MQ=255)                                                                                                                                 cGGTCGCGACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGccc       <  1:1018108/140‑1 (MQ=255)                                                                                                                                      gcgACTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGt  <  2:526976/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGATTACAGGTTGCTGCTATCGCCTTTCAGGCCGATCGGGAAGACTTCTTCCAGGTCGCCGTGCGGACGCGGGATAACGTGTACAGATACCAGCTCGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGT  >  NC_000913/2572154‑2572426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑