Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	229,324	G→A	G53D (GGC→GAC)	dkgB →	methylglyoxal reductase DkgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	229,324	0	G	A	100.0%	279.8 / NA	78	G53D (GGC→GAC)	dkgB	methylglyoxal reductase DkgB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/43);  total (35/43)

GGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGG  >  NC_000913/229191‑229460                                                                                                                                      |                                                                                                                                         ggTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGcc                                                                                                                                    >  2:307043/1‑140 (MQ=255)   tACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCAc                                                                                                                                  <  1:703505/140‑1 (MQ=255)   tACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCAc                                                                                                                                  <  1:946792/140‑1 (MQ=255)      tttCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTc                                                                                                                               >  1:811799/1‑140 (MQ=255)      tttCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTc                                                                                                                               <  2:637802/140‑1 (MQ=255)      tttCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTc                                                                                                                               >  2:322406/1‑140 (MQ=255)       ttCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCa                                                                                                                              >  1:325390/1‑140 (MQ=255)         ccGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATg                                                                                                                            <  2:550548/140‑1 (MQ=255)         ccGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATg                                                                                                                            <  1:307043/140‑1 (MQ=255)            tCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAAc                                                                                                                         <  2:836933/140‑1 (MQ=255)            tCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAAc                                                                                                                         <  1:758467/140‑1 (MQ=255)            tCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAAc                                                                                                                         <  2:414660/140‑1 (MQ=255)                aaaGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCa                                                                                                                              >  1:1108450/1‑131 (MQ=255)                aaaGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCa                                                                                                                              <  2:1108450/131‑1 (MQ=255)                   gacgacGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACAt                                                                                                                  <  2:327269/140‑1 (MQ=255)                   gacgacGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACAt                                                                                                                  <  2:811799/140‑1 (MQ=255)                   gacgacGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACAt                                                                                                                  <  1:139764/140‑1 (MQ=255)                   gacgacGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACAt                                                                                                                  <  2:949348/140‑1 (MQ=255)                     cgacgTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATca                                                                                                                <  1:1092368/140‑1 (MQ=255)                        cgTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATcacca                                                                                                             >  2:935233/1‑140 (MQ=255)                          ttgttATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACt                                                                                                           <  2:130799/140‑1 (MQ=255)                               aTTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGCGACGTGCCACGTCATGAACTCTACATCACCACTAAAAt                                                                                                      >  2:863056/1‑140 (MQ=255)                                 ttCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCt                                                                                                    <  2:774896/140‑1 (MQ=255)                                  tCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTg                                                                                                   >  1:974975/1‑140 (MQ=255)                                    aTCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGTAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGa                                                                                                 <  2:974975/140‑1 (MQ=255)                                       tgtgATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTg                                                                                              >  1:556921/1‑140 (MQ=255)                                       tgtgATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTg                                                                                              >  2:129106/1‑140 (MQ=255)                                         tgATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGaa                                                                                            >  1:724965/1‑140 (MQ=255)                                              aCGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAAtct                                                                                       <  1:225149/140‑1 (MQ=255)                                              aCGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAAtct                                                                                       >  2:44629/1‑140 (MQ=255)                                               cGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAAtctc                                                                                      >  2:207930/1‑140 (MQ=255)                                                   gcTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCa                                                                                  >  2:610904/1‑140 (MQ=255)                                                   gcTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCa                                                                                  >  1:1145107/1‑140 (MQ=255)                                                          cTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACaa                                                                           >  1:959007/1‑140 (MQ=255)                                                                   cgcgCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  <  2:325390/140‑1 (MQ=255)                                                                   cgcgCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  <  2:556921/140‑1 (MQ=255)                                                                     cgcAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCaa                                                                <  1:871647/140‑1 (MQ=255)                                                                     cgcAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGCGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCaa                                                                <  1:863056/140‑1 (MQ=255)                                                                       cAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGt                                                              >  2:177586/1‑140 (MQ=255)                                                                       cAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGt                                                              <  1:254358/140‑1 (MQ=255)                                                                              tACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAag                                                       >  2:546336/1‑140 (MQ=255)                                                                                 cGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAagaga                                                    <  1:272997/140‑1 (MQ=255)                                                                                  gCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  >  2:823545/1‑125 (MQ=255)                                                                                  gCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  <  1:823545/125‑1 (MQ=255)                                                                                  gCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAagagag                                                   <  2:846857/140‑1 (MQ=255)                                                                                   cacaAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGc                                                  >  2:1013431/1‑140 (MQ=255)                                                                                    acaAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  >  1:254799/1‑123 (MQ=255)                                                                                    acaAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATccc                                                                  <  2:254799/123‑1 (MQ=255)                                                                                    acaAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGcc                                                 >  2:1029030/1‑140 (MQ=255)                                                                                    acaAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGcc                                                 >  1:398759/1‑140 (MQ=255)                                                                                                tAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGc                                     >  2:162004/1‑140 (MQ=255)                                                                                                 aaCGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCg                                    <  1:322406/140‑1 (MQ=255)                                                                                                  aCGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGt                                   >  1:398466/1‑140 (MQ=255)                                                                                                  aCGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGt                                   <  2:469363/140‑1 (MQ=255)                                                                                                   cGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTa                                  >  1:208053/1‑140 (MQ=255)                                                                                                   cGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTa                                  >  1:904622/1‑140 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGt                                   <  1:51656/135‑1 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGt                                   >  2:51656/1‑135 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGa                              <  2:1145107/140‑1 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGa                              <  1:1013431/140‑1 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGa                              >  2:701065/1‑140 (MQ=255)                                                                                                       gCCGCAGTAGGTCAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGa                              <  1:935233/140‑1 (MQ=255)                                                                                                                   cAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  <  2:959007/140‑1 (MQ=255)                                                                                                                   cAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  <  1:546336/140‑1 (MQ=255)                                                                                                                   cAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  >  1:22758/1‑140 (MQ=255)                                                                                                                   cAGGCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  <  2:45806/140‑1 (MQ=255)                                                                                                                     ggCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                <  2:615022/140‑1 (MQ=255)                                                                                                                     ggCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                >  1:615022/1‑140 (MQ=255)                                                                                                                     ggCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                <  1:44629/140‑1 (MQ=255)                                                                                                                     ggCGATTACAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                >  2:524215/1‑140 (MQ=255)                                                                                                                      gCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  >  2:847636/1‑137 (MQ=255)                                                                                                                      gCGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCt                  <  1:847636/137‑1 (MQ=255)                                                                                                                       cGATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACg              <  1:903313/140‑1 (MQ=255)                                                                                                                        gATTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGc             >  1:707355/1‑140 (MQ=255)                                                                                                                         aTTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTTGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                <  2:495255/136‑1 (MQ=255)                                                                                                                         aTTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTTGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGa                >  1:495255/1‑136 (MQ=255)                                                                                                                         aTTGCAGAAAGTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCt            <  2:208053/140‑1 (MQ=255)                                                                                                                                   gTGACGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTgg  <  2:724965/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGATAACGGCGCTTGAACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGG  >  NC_000913/229191‑229460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑