Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,903	G→A	A34V (GCC→GTC)	eutQ ←	putative ethanolamine utilization protein EutQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,903	0	G	A	100.0%	201.1 / NA	57	A34V (GCC→GTC)	eutQ	putative ethanolamine utilization protein EutQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/25);  total (32/25)

TGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAA  >  NC_000913/2574768‑2575038                                                                                                                                        |                                                                                                                                        tGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTc                                                                                                                                     <  2:67628/140‑1 (MQ=255)       atgatgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGt                                                                                                                               >  1:621598/1‑140 (MQ=255)         gatgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAACCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGtaa                                                                                                                             <  2:1068455/140‑1 (MQ=255)         gatgGTTGCGCGGAGGCGCTGGCTTGCGGTTTTGTCGGCTGGCGCGCGGGGGGGCAGGGGCGCCGTTACGGGGATCGATTCATCACTTTCGGTGAGGGTAAACCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGtaa                                                                                                                             <  2:428136/140‑1 (MQ=255)          atgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGtaat                                                                                                                            >  1:677686/1‑140 (MQ=255)          atgGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGtaat                                                                                                                            >  1:524287/1‑140 (MQ=255)             gTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTaataat                                                                                                                         >  2:1033905/1‑140 (MQ=255)              tttCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATg                                                                                                                        >  2:415509/1‑140 (MQ=255)                 cgcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTCCCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTg                                                                                                                     <  1:865762/140‑1 (MQ=255)                 cgcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTGGGTGATGGAAAACCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTg                                                                                                                     <  2:22304/140‑1 (MQ=255)                  gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTgg                                                                                                                    >  1:1018466/1‑140 (MQ=255)                      aTGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGgcgcg                                                                                                                <  1:883790/140‑1 (MQ=255)                        gcggTGGCTTTCGGTTTTGTCGGCGGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCa                                                                                                              <  1:876523/140‑1 (MQ=255)                            tGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAAc                                                                                                          >  2:813889/1‑140 (MQ=255)                              gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCa                                                                                                        >  1:540774/1‑140 (MQ=255)                              gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCa                                                                                                        <  1:1033905/140‑1 (MQ=255)                                tttCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACg                                                                                                      >  2:313301/1‑140 (MQ=255)                                   cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAAcaa                                                                                                        <  2:804064/135‑3 (MQ=255)                                   cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAAcaa                                                                                                        >  1:804064/1‑133 (MQ=255)                                   cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGAc                                                                                                   >  1:627279/1‑140 (MQ=255)                                        ttGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAACCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAg                                                                                                             <  2:446107/125‑1 (MQ=255)                                        ttGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAg                                                                                                             >  1:446107/1‑125 (MQ=255)                                          gTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCt                                                                                            >  1:384426/1‑140 (MQ=255)                                                    aCGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCcgcg                                                                                  >  2:97912/1‑140 (MQ=255)                                                         ggCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCcgcgcgcgg                                                                             >  2:979677/1‑139 (MQ=255)                                                          gCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCgtg                                                                            <  2:384426/140‑1 (MQ=255)                                                            gggCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGtgtg                                                                          <  2:540774/140‑1 (MQ=255)                                                            gggCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGtgtg                                                                          <  1:313301/140‑1 (MQ=255)                                                               cACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCtt                                                                       <  1:682488/140‑1 (MQ=255)                                                                        cGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAatat                                                              >  1:346264/1‑140 (MQ=255)                                                                        cGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAatat                                                              >  2:318189/1‑140 (MQ=255)                                                                        cGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAat                                                                >  1:942323/1‑138 (MQ=255)                                                                        cGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAat                                                                <  2:942323/138‑1 (MQ=255)                                                                              cGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCg                                                        >  2:504987/1‑140 (MQ=255)                                                                                ggATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCt                                                      <  2:621598/140‑1 (MQ=255)                                                                                                 tCGGTGATGGTAAACCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCAc                                     <  1:973994/140‑1 (MQ=255)                                                                                                 tCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCAc                                     >  1:937893/1‑140 (MQ=255)                                                                                                 tCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCAc                                     >  2:973994/1‑140 (MQ=255)                                                                                                         ggTAAAACCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtcc                             >  2:246036/1‑140 (MQ=255)                                                                                                             aaaCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTcctcct                         >  1:1049165/1‑140 (MQ=255)                                                                                                               aCCCAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGt                       <  1:415509/140‑1 (MQ=255)                                                                                                                 ccAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGttt                     <  2:1049165/140‑1 (MQ=255)                                                                                                                  cAGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTg                    >  1:174583/1‑140 (MQ=255)                                                                                                                   aGTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTgg                   >  1:826769/1‑140 (MQ=255)                                                                                                                    gTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGt                  >  1:1019090/1‑140 (MQ=255)                                                                                                                    gTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGt                  <  2:937893/140‑1 (MQ=255)                                                                                                                    gTAGATCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGt                  >  1:878708/1‑140 (MQ=255)                                                                                                                        aTCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtc                              >  2:659990/1‑124 (MQ=255)                                                                                                                        aTCCGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTtc                              <  1:659990/124‑1 (MQ=255)                                                                                                                          ccGCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTgagaga            >  2:58186/1‑140 (MQ=255)                                                                                                                            gCCACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATg          <  2:524287/140‑1 (MQ=255)                                                                                                                              cACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGcc        >  1:665548/1‑140 (MQ=255)                                                                                                                              cACTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGcc        >  1:751142/1‑140 (MQ=255)                                                                                                                               aCTTCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCa       >  2:801681/1‑140 (MQ=255)                                                                                                                                  tCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGAt    <  2:627279/140‑1 (MQ=255)                                                                                                                                  tCACGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGAt    <  1:801681/140‑1 (MQ=255)                                                                                                                                    aCGGACTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATaa  <  2:1018466/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGGGCAATGATGGTTTCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCACGGACATTGCCTGTTCGCCGCGCGCGTGTGCTTCACGAATATCATTCGCTGTGATGAGTTTTTTCACCTGCTTCCTCCTGTTTGGTGAGAGATGCCAGATAA  >  NC_000913/2574768‑2575038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑