Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,624,198	G→A	S385S (TCG→TCA)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,198	0	G	A	100.0%	211.4 / NA	59	S385S (TCG→TCA)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

GCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCC  >  NC_000913/2624069‑2624332                                                                                                                                  |                                                                                                                                       gCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCatg                                                                                                                              >  2:1065615/1‑138 (MQ=255)   tctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCaatgat                                                                                                                                   >  1:65592/1‑133 (MQ=255)   tctATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCaatgat                                                                                                                                   <  2:65592/133‑1 (MQ=255)         atCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACAt                                                                                                                      <  1:606088/140‑1 (MQ=255)            cTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAAc                                                                                                                   >  1:188505/1‑140 (MQ=255)               aTCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCAcg                                                                                                                              <  1:1110068/126‑1 (MQ=255)               aTCACACCTTTGAGCATGTACTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTCGCGAAAGATTCACGCATCATCGACTCAATGATCCAcg                                                                                                                              >  2:1110068/1‑126 (MQ=255)                 cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTaa                                                                                                              >  1:1053508/1‑140 (MQ=255)                 cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTaa                                                                                                              >  1:116673/1‑140 (MQ=255)                 cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTaa                                                                                                              >  2:736839/1‑140 (MQ=255)                 cacaCCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTaa                                                                                                              >  2:1139016/1‑140 (MQ=255)                    acCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGaa                                                                                                           >  1:1030161/1‑140 (MQ=255)                     ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGaaa                                                                                                          >  2:607062/1‑140 (MQ=255)                     ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGaaa                                                                                                          >  1:356267/1‑140 (MQ=255)                     ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGaaa                                                                                                          >  2:726254/1‑140 (MQ=255)                       tttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGt                                                                                                        <  2:116673/140‑1 (MQ=255)                        ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTc                                                                                                       >  1:508819/1‑140 (MQ=255)                        ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTc                                                                                                       >  2:694937/1‑140 (MQ=255)                         tGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCa                                                                                                      >  1:1012026/1‑140 (MQ=255)                            gCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCg                                                                                                   <  1:607062/140‑1 (MQ=255)                              aTGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg                                                                                                 <  1:726254/140‑1 (MQ=255)                                gtgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                                               >  1:695441/1‑140 (MQ=255)                                gtgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                                               <  1:915108/140‑1 (MQ=255)                                gtgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                                               >  1:36775/1‑140 (MQ=255)                                gtgCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt                                                                                               <  1:1089483/140‑1 (MQ=255)                                  gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGGTTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGtggtgg                                                                                             >  1:665580/1‑140 (MQ=255)                                  gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt                                                                                                  <  1:917507/135‑1 (MQ=255)                                  gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt                                                                                                  >  2:917507/1‑135 (MQ=255)                                  gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGtggtgg                                                                                             >  2:390323/1‑140 (MQ=255)                                  gCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGtggtgg                                                                                             >  1:386212/1‑140 (MQ=255)                                   cTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTggtggt                                                                                            <  2:665580/140‑1 (MQ=255)                                   cTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTggtggt                                                                                            <  2:386212/140‑1 (MQ=255)                                   cTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTggtggt                                                                                            <  2:356267/140‑1 (MQ=255)                                     ggAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTg                                                                                          <  1:152789/140‑1 (MQ=255)                                            ctgcGTCAGGCTTCGTTCTACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACa                                                                                   >  1:559942/1‑140 (MQ=255)                                                    ggCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt                                                                           >  2:898935/1‑140 (MQ=255)                                                         cgttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgac                                                                      >  1:758692/1‑140 (MQ=255)                                                          gttcgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg                                                                     <  1:693657/140‑1 (MQ=255)                                                                cccGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGaagaag                                                               <  2:1012026/140‑1 (MQ=255)                                                                cccGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGaagaag                                                               <  2:559942/140‑1 (MQ=255)                                                                cccGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGaagaag                                                               <  1:1065615/140‑1 (MQ=255)                                                                          cTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa                                                     >  1:535071/1‑140 (MQ=255)                                                                          cTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa                                                     >  2:771819/1‑140 (MQ=255)                                                                          cTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa                                                     >  1:126641/1‑140 (MQ=255)                                                                                        tAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGcc                                       <  1:390323/140‑1 (MQ=255)                                                                                               tACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGa                                >  1:29835/1‑140 (MQ=255)                                                                                               tACCGCGTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGa                                >  1:1086118/1‑140 (MQ=255)                                                                                                    cgTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCa                            >  2:77467/1‑139 (MQ=255)                                                                                                    cgTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCa                            <  1:77467/139‑1 (MQ=255)                                                                                                    cgTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAg                           >  2:566501/1‑140 (MQ=255)                                                                                                    cgTGGCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAg                           >  2:255691/1‑140 (MQ=255)                                                                                                        gCGAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGt                       <  2:695441/140‑1 (MQ=255)                                                                                                          gAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACATGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGc                     <  2:1030161/140‑1 (MQ=255)                                                                                                          gAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGc                     <  2:758692/140‑1 (MQ=255)                                                                                                          gAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGc                     <  2:126641/140‑1 (MQ=255)                                                                                                          gAAAGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGc                     <  1:736839/140‑1 (MQ=255)                                                                                                            aaGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCAc                   <  2:36775/140‑1 (MQ=255)                                                                                                            aaGATTCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCATGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCAc                   <  2:188505/140‑1 (MQ=255)                                                                                                                ttCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTa               >  2:911125/1‑140 (MQ=255)                                                                                                                 tCACGCATCATCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAt              >  2:392706/1‑140 (MQ=255)                                                                                                                      catcatCGACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTAtcttct         <  2:508819/140‑1 (MQ=255)                                                                                                                             gACTCAATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGcc  <  1:1139016/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       GCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGAAGCGCCTGACCGAAGCAGGCGTGCACGTTATCTTCTCTGCGCC  >  NC_000913/2624069‑2624332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑