Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,904,216	G→A	intergenic (+842/+531)	ygcE → / ← queE	putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,904,216	0	G	A	100.0%	236.9 / NA	66	intergenic (+842/+531)	ygcE/queE	putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/35);  total (31/35)

ATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACG  >  NC_000913/2904080‑2904352                                                                                                                                         |                                                                                                                                         aTCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCaggg                                                                                                                                       >  2:92497/1‑140 (MQ=255)     ccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAaca                                                                                                                                   <  1:1027436/140‑1 (MQ=255)     ccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAaca                                                                                                                                   <  1:352170/140‑1 (MQ=255)        cTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTg                                                                                                                                >  2:497856/1‑140 (MQ=255)            cgcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGctc                                                                                                                            >  2:470709/1‑140 (MQ=255)                  gAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAc                                                                                                                      >  2:611997/1‑140 (MQ=255)                      acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAcagca                                                                                                                  <  2:775864/140‑1 (MQ=255)                      acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAcagca                                                                                                                  <  1:497856/140‑1 (MQ=255)                       ccTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAcagcag                                                                                                                 <  1:108527/140‑1 (MQ=255)                           ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCAcc                                                                                                             <  2:1144890/140‑1 (MQ=255)                             ttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCa                                                                                                           >  1:289716/1‑140 (MQ=255)                               cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAcag                                                                                                                    >  2:234360/1‑129 (MQ=255)                               cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAAcag                                                                                                                    <  1:234360/129‑1 (MQ=255)                                gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGa                                                                                                        >  1:138506/1‑140 (MQ=255)                                 cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGAc                                                                                                       <  1:160801/140‑1 (MQ=255)                                      cTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGt                                                                                                  <  1:899871/140‑1 (MQ=255)                                          ggACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                              <  2:12265/140‑1 (MQ=255)                                            aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGttt                                                                                            <  1:550592/140‑1 (MQ=255)                                              tATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt                                                                                          >  2:1075306/1‑140 (MQ=255)                                              tATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt                                                                                          >  2:601854/1‑140 (MQ=255)                                              tATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt                                                                                          >  1:837131/1‑140 (MQ=255)                                                ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATcc                                                                                        >  1:663689/1‑140 (MQ=255)                                                ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATcc                                                                                        >  2:705142/1‑140 (MQ=255)                                                ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATcc                                                                                        >  1:1105487/1‑140 (MQ=255)                                                   cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCg                                                                                     <  2:289716/140‑1 (MQ=255)                                                   cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCg                                                                                     >  2:1036466/1‑140 (MQ=255)                                                         ggTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGgcg                                                                               <  2:247879/140‑1 (MQ=255)                                                           tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             <  2:878651/140‑1 (MQ=255)                                                           tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             <  2:221126/140‑1 (MQ=255)                                                           tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             >  1:1124089/1‑140 (MQ=255)                                                           tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             <  2:1124089/140‑1 (MQ=255)                                                           tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             >  2:815101/1‑140 (MQ=255)                                                             tATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             >  1:757639/1‑138 (MQ=255)                                                             tATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                             <  2:757639/138‑1 (MQ=255)                                                                    gCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                    <  2:1105487/140‑1 (MQ=255)                                                                    gCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                    <  2:349023/140‑1 (MQ=255)                                                                    gCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                    <  1:611997/140‑1 (MQ=255)                                                                    gCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                    <  1:92497/140‑1 (MQ=255)                                                                          gcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGc                                                              <  2:580659/140‑1 (MQ=255)                                                                               gAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTg                                                         <  2:138506/140‑1 (MQ=255)                                                                               gAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTg                                                         <  1:967160/140‑1 (MQ=255)                                                                                          cACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGc                                              >  1:218215/1‑140 (MQ=255)                                                                                          cACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGc                                              >  1:45070/1‑140 (MQ=255)                                                                                            cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg                                            <  1:470709/140‑1 (MQ=255)                                                                                             ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGc                                           >  2:149603/1‑140 (MQ=255)                                                                                             ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGc                                           >  2:61664/1‑140 (MQ=255)                                                                                                  aCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAaca                                      <  2:837131/140‑1 (MQ=255)                                                                                                   cAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAacac                                     <  1:1036466/140‑1 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                             >  2:99839/1‑126 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                             <  1:99839/126‑1 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg                                            >  2:33287/1‑127 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg                                            <  1:33287/127‑1 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGttt                               >  1:88442/1‑140 (MQ=255)                                                                                                         cGTGTATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGttt                               >  2:730351/1‑140 (MQ=255)                                                                                                           tgtATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTAt                             >  2:1107539/1‑140 (MQ=255)                                                                                                           tgtATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTAt                             >  1:1098597/1‑140 (MQ=255)                                                                                                             tATCTGCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc                           >  2:418548/1‑140 (MQ=255)                                                                                                                  gCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCt                      >  1:768967/1‑140 (MQ=255)                                                                                                                  gCTCGGTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCt                      >  2:253213/1‑140 (MQ=255)                                                                                                                       gTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgcgc                 <  1:601854/140‑1 (MQ=255)                                                                                                                       gTTTATCCCCGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgcgc                 <  1:1075306/140‑1 (MQ=255)                                                                                                                             ccccGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa           >  2:603703/1‑140 (MQ=255)                                                                                                                             ccccGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa           <  1:61664/140‑1 (MQ=255)                                                                                                                             ccccGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa           <  1:603703/140‑1 (MQ=255)                                                                                                                             ccccGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa           <  1:149603/140‑1 (MQ=255)                                                                                                                             ccccGCTGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa           >  1:134589/1‑140 (MQ=255)                                                                                                                                   tGGCGCAGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACAtt     <  2:218215/140‑1 (MQ=255)                                                                                                                                      cgcaGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACg  <  2:264481/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACG  >  NC_000913/2904080‑2904352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑