Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,918,991	A→G	V132A (GTG→GCG)	gudD ←	D‑glucarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,918,991	0	A	G	100.0%	274.7 / NA	74	V132A (GTG→GCG)	gudD	D‑glucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/40);  total (34/40)

AGGCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCACGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGC  >  NC_000913/2918856‑2919125                                                                                                                                        |                                                                                                                                       aGGCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGtt                                                                                                                                    >  1:1039101/1‑140 (MQ=255) aGGCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGtt                                                                                                                                    >  2:315279/1‑140 (MQ=255)  ggCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGttt                                                                                                                                   <  2:1039101/140‑1 (MQ=255)  ggCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGttt                                                                                                                                   >  2:661296/1‑140 (MQ=255)             tCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtg                                                                                                                        <  2:74315/140‑1 (MQ=255)             tCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtg                                                                                                                        >  1:909576/1‑140 (MQ=255)             tCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtg                                                                                                                        >  1:913273/1‑140 (MQ=255)             tCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtg                                                                                                                        >  1:225374/1‑140 (MQ=255)                 aTGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtgctgc                                                                                                                    >  1:1034684/1‑140 (MQ=255)                 aTGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGAtgctgc                                                                                                                    >  2:507077/1‑140 (MQ=255)                  tGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGcc                                                                                                                   >  1:566802/1‑140 (MQ=255)                  tGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGcc                                                                                                                   >  1:483203/1‑140 (MQ=255)                    aGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGcccc                                                                                                                 >  1:600695/1‑140 (MQ=255)                      tCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCcag                                                                                                               <  1:277732/140‑1 (MQ=255)                       cATCCGGCTGGCTTTGATACGGCAGCGGCGTGACTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCcagc                                                                                                              <  1:1039393/140‑1 (MQ=255)                         tCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCcagcag                                                                                                            >  2:1063660/1‑140 (MQ=255)                             gctggctTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGCTGACCATCCCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATcc                                                                                                        >  2:681795/1‑140 (MQ=255)                              ctggctTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCa                                                                                                       >  2:312928/1‑140 (MQ=255)                                  ctTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCAt                                                                                                   >  1:777093/1‑140 (MQ=255)                                     tGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGc                                                                                                <  2:160452/140‑1 (MQ=255)                                           ggCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTc                                                                                          >  1:179467/1‑140 (MQ=255)                                              aGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTAt                                                                                       <  2:566802/140‑1 (MQ=255)                                                  gcgTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCg                                                                                   <  2:580091/140‑1 (MQ=255)                                                    gTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGt                                                                                 <  2:483203/140‑1 (MQ=255)                                                     tGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTa                                                                                >  2:586285/1‑140 (MQ=255)                                                     tGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTa                                                                                <  2:600695/140‑1 (MQ=255)                                                     tGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCAGTa                                                                                <  2:541532/140‑1 (MQ=255)                                                       gCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTCCGCTGTTGGCCATCGCCCAGCAGCGATGCCGCGTTTGCCCCCAGATGCTGCCCCAGCAGATCCGGCATTGCCGCTTCTATCCCGGTAAc                                                                              <  2:993665/140‑1 (MQ=255)                                                       gCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAAc                                                                              <  2:932757/140‑1 (MQ=255)                                                       gCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAAc                                                                              >  2:746344/1‑140 (MQ=255)                                                       gCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAAc                                                                              <  1:253053/140‑1 (MQ=255)                                                       gCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAAc                                                                              <  2:1034684/140‑1 (MQ=255)                                                          ttGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTAc                                                                           <  1:661296/140‑1 (MQ=255)                                                            gCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACAt                                                                         >  1:138188/1‑140 (MQ=255)                                                             cGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCGCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATg                                                                        <  1:785759/140‑1 (MQ=255)                                                             cGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATg                                                                        <  2:225374/140‑1 (MQ=255)                                                              gATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGa                                                                       <  2:777093/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  1:746344/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  1:1063660/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  1:180236/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  2:516136/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  2:1119740/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  2:138188/140‑1 (MQ=255)                                                               aTTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGaa                                                                      <  2:179467/140‑1 (MQ=255)                                                                 tACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATa                                                                    <  2:913273/140‑1 (MQ=255)                                                                  aCCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAg                                                                   <  2:667648/140‑1 (MQ=255)                                                                  aCCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAg                                                                   >  1:897519/1‑140 (MQ=255)                                                                         aagaaCAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACg                                                            <  1:411418/140‑1 (MQ=255)                                                                            aaCAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAg                                                         >  2:106137/1‑140 (MQ=255)                                                                             aCAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAgg                                                        >  2:639387/1‑140 (MQ=255)                                                                              cAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGt                                                       <  1:639387/140‑1 (MQ=255)                                                                                   aaCCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAAt                                                  >  1:858552/1‑140 (MQ=255)                                                                                    aCCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATg                                                 <  1:507077/140‑1 (MQ=255)                                                                                      cGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTa                                                              >  2:160263/1‑125 (MQ=255)                                                                                      cGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTa                                                              <  1:160263/125‑1 (MQ=255)                                                                                      cGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTc                                               >  1:809240/1‑140 (MQ=255)                                                                                         gCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGc                                            >  2:1077319/1‑140 (MQ=255)                                                                                          cATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCa                                           <  1:315279/140‑1 (MQ=255)                                                                                                 aCTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACcgcg                                    <  2:96465/140‑1 (MQ=255)                                                                                                 aCTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACcgcg                                    <  1:312928/140‑1 (MQ=255)                                                                                                   ttCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGcc                                  <  1:106137/140‑1 (MQ=255)                                                                                                      gCTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCccac                               <  2:540851/140‑1 (MQ=255)                                                                                                       cTACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCccacc                              >  2:843256/1‑140 (MQ=255)                                                                                                        tACGCTGTTGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCccacca                             <  2:858552/140‑1 (MQ=255)                                                                                                               ttGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCAc                      >  2:687363/1‑140 (MQ=255)                                                                                                               ttGACCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCAc                      >  1:205119/1‑140 (MQ=255)                                                                                                                  aCCATCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGAt                   <  1:1077319/140‑1 (MQ=255)                                                                                                                     aTCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCGCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCgg                <  2:897519/140‑1 (MQ=255)                                                                                                                     aTCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCgg                >  1:39339/1‑140 (MQ=255)                                                                                                                     aTCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCgg                >  1:492808/1‑140 (MQ=255)                                                                                                                      tCGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGc               >  2:1140423/1‑140 (MQ=255)                                                                                                                       cGCCCAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCa              >  2:19229/1‑140 (MQ=255)                                                                                                                         cccAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCaaa            <  2:809240/140‑1 (MQ=255)                                                                                                                         cccAGCAGCGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCaaa            >  2:91299/1‑140 (MQ=255)                                                                                                                             gcagcGATGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTa        <  2:909576/140‑1 (MQ=255)                                                                                                                                   aTGCCGCGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGc  >  1:333294/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       AGGCGATACCAGTCGCATGAGTCATCCGGCTGGCTTTGATACGGCAGCGGCGTGGCTTTGCGATTACCGACGAAGAACAGATAACCGAGCATTTCGACTTCGCTACGCTGTTGACCATCGCCCAGCAGCGATGCCACGTTTACCCCCAGATGCTGCCCCAGCAGATCCAGCATTGCCGCTTCTATCCCGGTAACTACATGAATAGTGGTACGTAGGTCAAATGTCTGCAAACCGCGCCCACCAGCATCACGATCGGCAAAAGTATTACGC  >  NC_000913/2918856‑2919125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑