Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	232,955	T→C	D334G (GAC→GGC)	mltD ←	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,955	0	T	C	100.0%	234.6 / NA	64	D334G (GAC→GGC)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/38);  total (26/38)

TGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGT  >  NC_000913/232822‑233090                                                                                                                                      |                                                                                                                                        tGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCaa                                                                                                                                   <  1:500937/140‑1 (MQ=255)  ggCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAAc                                                                                                                                  <  2:87773/140‑1 (MQ=255)   gCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAAcc                                                                                                                                 >  1:334306/1‑140 (MQ=255)      tCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGc                                                                                                                              <  1:263083/140‑1 (MQ=255)           ttAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGa                                                                                                                         <  1:228538/140‑1 (MQ=255)           ttAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCAAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGa                                                                                                                         <  2:824013/140‑1 (MQ=255)             aGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGc                                                                                                                              <  1:731946/133‑1 (MQ=255)             aGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGc                                                                                                                              >  2:731946/1‑133 (MQ=255)               aTCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGt                                                                                                                     >  2:1084805/1‑140 (MQ=255)                   gcgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTAcag                                                                                                                 >  2:322506/1‑140 (MQ=255)                    cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTTAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTAcagc                                                                                                                >  1:38343/1‑140 (MQ=255)                    cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGt                                                                                                                            >  2:443541/1‑128 (MQ=255)                    cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGt                                                                                                                            <  1:443541/128‑1 (MQ=255)                    cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTAcagc                                                                                                                >  1:949387/1‑140 (MQ=255)                    cgcAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTAcagc                                                                                                                >  2:1126398/1‑140 (MQ=255)                                cACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGcc                                                                                                    <  1:1126398/140‑1 (MQ=255)                                 aCTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCt                                                                                                   <  1:1106460/140‑1 (MQ=255)                                   tgctgcAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGa                                                                                                 >  1:846640/1‑140 (MQ=255)                                     ctgcAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGcc                                                                                                    >  1:207401/1‑135 (MQ=255)                                     ctgcAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGcc                                                                                                    <  2:207401/135‑1 (MQ=255)                                       gcAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGcc                                                                                             <  1:812757/140‑1 (MQ=255)                                                tGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCa                                                                                    <  1:568310/140‑1 (MQ=255)                                                  gTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACg                                                                                  <  1:322506/140‑1 (MQ=255)                                                   tGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGc                                                                                 <  1:1084805/140‑1 (MQ=255)                                                     cTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAg                                                                               <  1:320666/140‑1 (MQ=255)                                                       tACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGtt                                                                             <  2:741445/140‑1 (MQ=255)                                                       tACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGtt                                                                             >  2:386725/1‑140 (MQ=255)                                                       tACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGtt                                                                             <  1:398073/140‑1 (MQ=255)                                                        aCGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTg                                                                            <  2:923180/140‑1 (MQ=255)                                                          gCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGAt                                                                          <  2:657213/140‑1 (MQ=255)                                                                aCGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCAt                                                                    >  1:912052/1‑140 (MQ=255)                                                                  gTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGc                                                                  <  1:859783/140‑1 (MQ=255)                                                                   tGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGct                                                                 <  2:846640/140‑1 (MQ=255)                                                                         gATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTg                                                           <  1:798328/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:949387/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:255461/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:878249/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:912052/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:1120332/140‑1 (MQ=255)                                                                            aCTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  1:793593/140‑1 (MQ=255)                                                                                gAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCAt                                                    >  1:55254/1‑140 (MQ=255)                                                                                    gCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACg                                                <  1:386725/140‑1 (MQ=255)                                                                                     cGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  1:1148281/131‑1 (MQ=255)                                                                                     cGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        >  2:1148281/1‑131 (MQ=255)                                                                                     cGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        <  2:120068/131‑1 (MQ=255)                                                                                     cGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCa                                                        >  1:120068/1‑131 (MQ=255)                                                                                       tgtCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTAc                                             >  1:885887/1‑140 (MQ=255)                                                                                       tgtCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTAc                                             >  1:543981/1‑140 (MQ=255)                                                                                       tgtCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTAc                                             >  1:575861/1‑140 (MQ=255)                                                                                        gtCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACt                                            >  1:912492/1‑140 (MQ=255)                                                                                              agagCGTACGGTGTAAACACGGCTTTTAAGCGGCGTATCGCCCACAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCCCCATCACGTACTGCGGGc                                      <  2:1063114/140‑1 (MQ=255)                                                                                              agagCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGc                                      <  2:885887/140‑1 (MQ=255)                                                                                              agagCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGc                                      <  2:108461/140‑1 (MQ=255)                                                                                                            aaaCACGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGc                        >  2:824583/1‑140 (MQ=255)                                                                                                                 cGGCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGa                   <  2:1084605/140‑1 (MQ=255)                                                                                                                  ggCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAg                  <  2:55254/140‑1 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAg                  >  2:479433/1‑139 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAg                  <  1:479433/139‑1 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  1:449718/1‑140 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  1:21733/1‑140 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  2:37658/1‑140 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  1:119845/1‑140 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  2:1010840/1‑140 (MQ=255)                                                                                                                   gCTGTTAAGCGGCGTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGc                 >  1:1017771/1‑140 (MQ=255)                                                                                                                            cggcgTATTGCCGGCAACCAGCGTCGACTGTACAGCAGCTATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGc        <  2:334306/140‑1 (MQ=255)                                                                                                                                  aTTGCCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGt  <  1:1010840/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGGCTTCAGCTTAGATCCGCGCAGTTTGTTCCACTGCTGCAAATCTTTGGTGCTTACGCCGAGACGTGAAGCGATACTTGAAAGCGTGTCGCCAGAGCGTACGGTGTAAACACGGCTGTTAAGCGGCGTATTGTCGGCAACCAGCGTCGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCCTTTTACGCCAGCGT  >  NC_000913/232822‑233090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑