Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,944	T→C	G145G (GGT→GGC)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,944	0	T	C	100.0%	152.8 / NA	43	G145G (GGT→GGC)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

TGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACG  >  NC_000913/3007808‑3008079                                                                                                                                         |                                                                                                                                        tGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGctct                                                                                                                                      >  2:605033/1‑140 (MQ=255) tGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGctct                                                                                                                                      >  2:1024255/1‑140 (MQ=255) tGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGctct                                                                                                                                      <  1:301590/140‑1 (MQ=255)  gTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTg                                                                                                                                     >  2:465029/1‑140 (MQ=255)   tCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGc                                                                                                                                    >  2:641250/1‑140 (MQ=255)   tCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGc                                                                                                                                    >  1:1145161/1‑140 (MQ=255)             aCCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAAcgc                                                                                                                          >  2:207226/1‑140 (MQ=255)               cTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGt                                                                                                                        >  1:1047857/1‑140 (MQ=255)                 gAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTg                                                                                                                      >  1:895858/1‑140 (MQ=255)                                        tGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGcc                                                                                               <  1:641250/140‑1 (MQ=255)                                              tCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGc                                                                                         <  1:977299/140‑1 (MQ=255)                                                     caccaccGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGGGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCa                                                                                  >  2:877938/1‑140 (MQ=255)                                                              cGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGa                                                                         >  2:210339/1‑140 (MQ=255)                                                                          gcgcGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATa                                                             >  2:745693/1‑140 (MQ=255)                                                                           cgcgGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATAc                                                            >  2:724598/1‑140 (MQ=255)                                                                             cgGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCg                                                          <  1:917846/140‑1 (MQ=255)                                                                                      gTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGa                                                 <  1:249119/140‑1 (MQ=255)                                                                                         ggCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCa                                              >  1:234178/1‑140 (MQ=255)                                                                                          gcagcaCAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCAt                                             >  2:189753/1‑140 (MQ=255)                                                                                              cacagcaACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGcc                                                    <  2:78919/129‑1 (MQ=255)                                                                                              cacagcaACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGcc                                                    >  1:78919/1‑129 (MQ=255)                                                                                              cacagcaACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCaa                                         >  1:923879/1‑140 (MQ=255)                                                                                               acagcaACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAac                                        <  1:189753/140‑1 (MQ=255)                                                                                                 agcaACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAacac                                      >  1:696298/1‑140 (MQ=255)                                                                                                   caACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCa                                          >  1:8288/1‑134 (MQ=255)                                                                                                   caACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCa                                          <  2:8288/134‑1 (MQ=255)                                                                                                    aaCTCGGACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACAcgcg                                   <  2:895858/140‑1 (MQ=255)                                                                                                          gACAGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCgcagcag                             <  2:504555/140‑1 (MQ=255)                                                                                                             aGAATGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCAc                          <  2:607994/140‑1 (MQ=255)                                                                                                               aaTGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACTATGCAACACGCGCAGCAGCACgg                        >  1:663308/1‑140 (MQ=255)                                                                                                                 tGCGGTGATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCt                      >  2:384363/1‑140 (MQ=255)                                                                                                                       gATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  2:575729/140‑1 (MQ=255)                                                                                                                       gATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  2:923879/140‑1 (MQ=255)                                                                                                                       gATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  1:465029/140‑1 (MQ=255)                                                                                                                       gATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  1:605033/140‑1 (MQ=255)                                                                                                                       gATTTACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCATTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  2:888031/140‑1 (MQ=255)                                                                                                                         tttACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                <  1:1102133/138‑1 (MQ=255)                                                                                                                         tttACATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAg                >  2:1102133/1‑138 (MQ=255)                                                                                                                            aCATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTa           <  1:207226/140‑1 (MQ=255)                                                                                                                            aCATGCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATAACGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTa           <  2:1145161/140‑1 (MQ=255)                                                                                                                                gCCGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAgg       >  2:562205/1‑140 (MQ=255)                                                                                                                                  cGAAAGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGac     >  2:475290/1‑140 (MQ=255)                                                                                                                                    aaaGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGacac   >  1:123852/1‑140 (MQ=255)                                                                                                                                    aaaGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGacac   <  1:791139/140‑1 (MQ=255)                                                                                                                                     aaGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACg  <  1:1024255/140‑1 (MQ=255)                                                                                                                                     aaGGCTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACg  <  1:877938/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TGTCATTTGAGCACCTGAAAAATGCCATCGGCGAAAAAATGACTTTCGCGACCACCACCGACGGCAACCACGGGCGCGGTGTGGCGTGGGCAGCACAGCAACTCGGACAGAATGCGGTGATTTACATGCCGAAAGGTTCTGCTCAGGAACGCGTTGACGCCATTCTGAACCTCGGTGCCGAGTGCATCGTCACGGATATGAACTATGACGATACCGTTCGCCTGACCATGCAACACGCGCAGCAGCACGGCTGGGAAGTGGTACAGGACACG  >  NC_000913/3007808‑3008079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑