Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,650	G→A	A434T (GCT→ACT)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,650	0	G	A	100.0%	137.0 / NA	39	A434T (GCT→ACT)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/20);  total (19/20)

ACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACA  >  NC_000913/3025519‑3025782                                                                                                                                    |                                                                                                                                      aCGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCggg                                                                                                                               >  1:851692/1‑140 (MQ=255)  cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  1:324988/1‑140 (MQ=255)  cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  1:389056/1‑140 (MQ=255)  cGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTAGTAGTTGAAAACCCTATTTGTACTGGCGGGt                                                                                                                              >  2:705810/1‑140 (MQ=255)       tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑t                                                                                                                        >  2:829369/1‑140 (MQ=255)       tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑t                                                                                                                        <  2:581794/140‑1 (MQ=255)           gcgcCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGa                                                                                                                    <  2:324988/140‑1 (MQ=255)            cgcCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGAt                                                                                                                   <  2:324186/140‑1 (MQ=255)                           aTTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAC‑TTCGCTTTTTTTAAAtattat                                                                                                    >  2:1064432/1‑140 (MQ=255)                           aTTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGCCCCAC‑CCCGCTTTTTTTTAAtattat                                                                                                    >  2:793366/1‑140 (MQ=255)                                   cACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCtg                                                                                            <  1:818844/140‑1 (MQ=255)                                             ggtttaggGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCg                                                                                  <  2:310372/140‑1 (MQ=255)                                                 taggGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTTAT‑TTCGTTTTTTTTTTAAATTTTTCTCCCCGGTGGGTACCGACaa                                                                              >  2:644298/1‑139 (MQ=255)                                                        tGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TTCGATTTTTTTTAATATTATTCTCCCCCGTGGCTACCCACAGGaaaaaa                                                                       >  2:222224/1‑138 (MQ=255)                                                          gCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGtt                                                                     <  2:125635/140‑1 (MQ=255)                                                             tctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTg                                                                  <  1:974774/140‑1 (MQ=255)                                                              ctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGc                                                                 <  1:1064432/140‑1 (MQ=255)                                                              ctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGc                                                                 <  1:705810/140‑1 (MQ=255)                                                               tcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTTACTTGGGATTTT‑TTTAATATTATTCTCCCTCGGGGGTACCCACCGGAAAACAATCTGcc                                                                >  2:176812/1‑140 (MQ=255)                                                               tcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGcc                                                                <  1:176812/140‑1 (MQ=255)                                                                         ccGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TTCGGTTTTTTTTAATATTTTTCTCCCCCGTGGGTACCCACAGGAAAAAATTCTGCCTGGGAttttc                                                      >  2:559156/1‑137 (MQ=255)                                                                          cGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCt                                                     <  1:222224/140‑1 (MQ=255)                                                                              aTTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGc                                                 <  1:967189/140‑1 (MQ=255)                                                                                      aaTATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAgaga                                         <  1:1050885/140‑1 (MQ=255)                                                                                           tGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TTCGATTTTATTTAATATTATTCTCCCCTGTGGCTACCCACAGGAAAAAGTTCTGCCCTGTATTTTCTCAGCGGGAGAGATgggt                                    >  2:854120/1‑138 (MQ=255)                                                                                            gCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAtt                                   <  1:990122/140‑1 (MQ=255)                                                                                            gCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAtt                                   <  2:851692/140‑1 (MQ=255)                                                                                                 ccTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTTAA‑TGCGAGTTTTTTTAATATTATTCTCCCCTCTGGCTACCGACCAGAAAACGTTTTTCCTGGTATTACCTCCGCGGGAGAGAAGGTTTaaaaa                              >  2:990122/1‑137 (MQ=255)                                                                                                 ccTCTATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TGCGGTTTTTTTTAATATTATTCTCCCCGGTGGGTACCGCCAGGAAAAAGTTCTGCCTGGTATTTCCTCCACGGGAGAGAAGGATTaaaaa                              >  2:1050885/1‑137 (MQ=255)                                                                                                    ctATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAA‑TGCGGTTTTTTTTAATATTATTCTCCCTCGTGGGTACCCGCCGGAAAAAGGTTTTCCTGGTATTTCCTCAGCGGGAGAGAAGGGTTaaaaaaaa                           >  2:837825/1‑134 (MQ=255)                                                                                                     tATTTATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTaaa                          <  1:793366/140‑1 (MQ=255)                                                                                                        ttATGTATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGa                       <  2:389056/140‑1 (MQ=255)                                                                                                           tGTATTAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TGCGATTTTTTTTAATATTATTCTCCCTTGTGGGTACCGACAAGAAAAAGGTCTGCCTGGTATTACCTCCGCGGGAGAGATGGGTTAAAAGTAAAGGAggc                    >  2:978120/1‑138 (MQ=255)                                                                                                             tATTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCaa                  <  1:829369/140‑1 (MQ=255)                                                                                                              aTTAGTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAat                 <  1:644298/140‑1 (MQ=255)                                                                                                               ttAGTTGAAAACCCTATTTGTACTGGCGGGGTAAA‑TTCCGTTTTTTTTAATATTATTCTCCCTCGGGGCTACCGCCAGGAAAACGTTCTGCCTGGTATTTCCTCCCCGGAAGAGAGGGGGTAACAGAAAAAGAGGCaaag                >  2:1126395/1‑138 (MQ=255)                                                                                                                 aGTTGAAAACCCTATTTGTACTGGCGGCTTTAA‑TGCGATTTTTTTTAAAATTATCCTCCCCGGTGGGTGCCGACAGGGAAACCTTTCTCCTTGTTTTTCCTCCGCGGAAGAGAGGGATTTAAAATAAAAGAGTCCatgag              >  2:508334/1‑139 (MQ=255)                                                                                                                  gTTGAAAACCCTATTTGTACTGGCGGGTTAAC‑TGCGGTTTTTTTTAATATTATCCTCCCTTGTGGCTACCGACAGGAAAAAAGTTTTCCTGGTATTATCTCAGCGGGAGAGATGGATTAACAGTAAAAGAGGCAataatg             >  2:737085/1‑140 (MQ=255)                                                                                                                             tATTTGTACTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAaca  <  1:634983/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAC‑TGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACA  >  NC_000913/3025519‑3025782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑