Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,033,306	T→A	I81N (ATC→AAC)	idi →	isopentenyl‑diphosphate Delta‑isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,033,306	0	T	A	100.0%	179.8 / NA	56	I81N (ATC→AAC)	idi	isopentenyl‑diphosphate Delta‑isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (38/18);  total (38/18)

CTCCAGTTGGCTGTTTAATGCCAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCAC  >  NC_000913/3033169‑3033441                                                                                                                                          |                                                                                                                                        ctcCAGTTGGCTGTTTAATGCCAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAAcc                                                                                                                                       >  2:183791/1‑140 (MQ=255)   ccgtttGGCTGTTAAGTGCAAAAGAAAATTTATTTGTTCCCCCCCCCGCACTGAGAAAAAAAGCTGGGCCGGGGGGGGGGACAACCTCGGTTTGTGGGCACCCACAGCGGGGAAAAAGCAACGAAGACGCAGTGAAccgc                                                                                                                                     <  2:447449/136‑1 (MQ=255)     gtttGGTTTTTAAATGCAAAAGAAAAATTATTATTTCCCCGCCGCGCACTGAGAAAAAAAGCATGGCCGGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAAccgccg                                                                                                                                   <  2:471626/138‑1 (MQ=255)                        aaGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGc                                                                                                                >  1:958966/1‑140 (MQ=255)                                   ttaTTTCCCCGCCGCGCACTGAGAAAAAAAGCATGGCCTGGCGTGTGGACTACCTCGGTTTGTGGGCCCCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTAc                                                                                                     <  1:984166/140‑1 (MQ=255)                                    tgttcccccGCCGCGCACTGAGAAAAAAAGCATGGCCGGGGGTGTGGACAAGCTGGGTTTGGGGGCCCCCACAAGGGGGAGAAAGCAAAAAAGCCGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACg                                                                                                    <  2:1138176/134‑1 (MQ=255)                                    taGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTACCTCGGTTTGTGGGCCCCCACAACTGGGAAAAAGCAACGAAGACGCGGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACg                                                                                                    <  2:78272/140‑1 (MQ=255)                                    taGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACg                                                                                                    <  1:511244/140‑1 (MQ=255)                                          cccGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGcctcct                                                                                              >  1:989870/1‑140 (MQ=255)                                          cccGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGcctcct                                                                                              >  2:902111/1‑140 (MQ=255)                                          cccGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGcctcct                                                                                              >  2:206890/1‑140 (MQ=255)                                          cccGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGcctcct                                                                                              >  1:865197/1‑140 (MQ=255)                                          cccGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGC‑‑GGAAATTACGCCTCCTGa                                                                                            >  2:853057/1‑140 (MQ=255)                                           ccgccgCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGAGGAAATTACGCCTCCTg                                                                                             >  2:180545/1‑140 (MQ=255)                                              ccgcGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAat                                                                                          >  2:1107439/1‑140 (MQ=255)                                                 cgcACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAatcta                                                                                       >  2:199904/1‑140 (MQ=255)                                                 cgcACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAatcta                                                                                       >  2:307238/1‑140 (MQ=255)                                                                   aTGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCt                                                                     <  1:183791/140‑1 (MQ=255)                                                                    tGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTa                                                                    >  1:537290/1‑140 (MQ=255)                                                                       ccTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACcg                                                                 <  1:1008769/140‑1 (MQ=255)                                                                           gCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGcc                                                             >  1:11581/1‑140 (MQ=255)                                                                                tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGa                                                        >  1:570587/1‑140 (MQ=255)                                                                                tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGa                                                        >  2:952069/1‑140 (MQ=255)                                                                                tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGa                                                        >  1:419646/1‑140 (MQ=255)                                                                                tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGa                                                        >  2:233047/1‑140 (MQ=255)                                                                                tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGa                                                        >  1:236266/1‑140 (MQ=255)                                                                                 ggACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACAAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGAt                                                       <  1:329337/140‑1 (MQ=255)                                                                                  gACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACcg                                                                 <  1:934317/129‑1 (MQ=255)                                                                                  gACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACcg                                                                 >  2:934317/1‑129 (MQ=255)                                                                                     tAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGa                                                   >  1:1114824/1‑140 (MQ=255)                                                                                        cTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTg                                                >  1:1058452/1‑140 (MQ=255)                                                                                            gTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCAt                                            >  2:680566/1‑140 (MQ=255)                                                                                            gTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCAt                                            <  1:749763/140‑1 (MQ=255)                                                                                             tttGTGGGCCCCCACAACTGGGAGAAGGCAACGAAGACGCAGTAAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCAtt                                           <  2:537290/140‑1 (MQ=255)                                                                                               tgtgGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATtgt                                         >  1:290806/1‑140 (MQ=255)                                                                                               tgtgGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATtgt                                         >  1:170105/1‑140 (MQ=255)                                                                                                 tgGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCAtt                                           >  1:47158/1‑136 (MQ=255)                                                                                                 tgGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCAtt                                           <  2:47158/136‑1 (MQ=255)                                                                                                 ggggCCCCCACAACGGGGAGAAAGCAACGAAGACGCGGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTgg                                       <  2:79118/139‑1 (MQ=255)                                                                                                 ggggCACCCACAACTGGGAGAAAGCAACAAAGACGCAGTAAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTgg                                       <  2:989870/139‑1 (MQ=255)                                                                                                   ggCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGaa                                     >  2:266285/1‑140 (MQ=255)                                                                                                    gcccccACAACGGGGAAAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGaaa                                    <  2:170105/137‑1 (MQ=255)                                                                                                    gCACCCACAACTGGGAAAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGaaa                                    <  1:372938/140‑1 (MQ=255)                                                                                                           caACTGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAgt                             <  2:419646/140‑1 (MQ=255)                                                                                                               tGGGAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAgtgtgt                         <  2:1073534/140‑1 (MQ=255)                                                                                                                 ggAGAAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTcc                       >  1:705538/1‑140 (MQ=255)                                                                                                                  gagaAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCg                      >  2:1015702/1‑140 (MQ=255)                                                                                                                  gagaAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCg                      >  1:899489/1‑140 (MQ=255)                                                                                                                  gagaAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCg                      >  1:73841/1‑140 (MQ=255)                                                                                                                  gagaAAGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCg                      >  2:1126177/1‑140 (MQ=255)                                                                                                                      aaGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTAt                  >  1:98233/1‑140 (MQ=255)                                                                                                                      aaGCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTAt                  >  2:886274/1‑140 (MQ=255)                                                                                                                        gCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTAttt                >  1:138447/1‑140 (MQ=255)                                                                                                                        gCAACGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTAttt                >  2:577615/1‑140 (MQ=255)                                                                                                                           aCGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGcc             <  1:1015702/140‑1 (MQ=255)                                                                                                                           aCGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGcc             >  2:1098002/1‑140 (MQ=255)                                                                                                                            cGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcg            <  1:1107439/140‑1 (MQ=255)                                                                                                                            cGAAGACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcg            <  2:236266/140‑1 (MQ=255)                                                                                                                              aggccgcgGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcgca          <  2:985695/136‑1 (MQ=255)                                                                                                                              aaGATGAAGTAATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcgca          <  1:180545/140‑1 (MQ=255)                                                                                                                              aaGACGCAGGGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcgca          <  1:902111/140‑1 (MQ=255)                                                                                                                                gACGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcgcacg        <  1:233047/140‑1 (MQ=255)                                                                                                                                 aCGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcg            <  2:1118152/135‑1 (MQ=255)                                                                                                                                 aCGCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCcg            >  1:1118152/1‑135 (MQ=255)                                                                                                                                   gCAGTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCgcacgcac     >  2:558597/1‑140 (MQ=255)                                                                                                                                      gTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac  <  1:577615/140‑1 (MQ=255)                                                                                                                                      gTGAACCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGcaccac  <  1:266285/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CTCCAGTTGGCTGTTTAATGCCAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTATGAGCTTGGCGTGGAAATTACGCCTCCTGAATCTATCTATCCTGACTTTCGCTACCGCGCCACCGATCCGAGTGGCATTGTGGAAAATGAAGTGTGTCCGGTATTTGCCGCACGCACCAC  >  NC_000913/3033169‑3033441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑