Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,154,296	T→C	D308G (GAT→GGT)	yqhC ←	DNA‑binding transcriptional activator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,154,296	0	T	C	100.0%	185.8 / NA	50	D308G (GAT→GGT)	yqhC	DNA‑binding transcriptional activator YqhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

CCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATCCGCGCCTTATGCAG  >  NC_000913/3154164‑3154429                                                                                                                                     |                                                                                                                                      ccGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTcccc                                                                                                                                <  2:924280/140‑1 (MQ=255)  cGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTccccc                                                                                                                               >  2:365231/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGTAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          >  2:582197/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          >  2:72808/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          >  2:240332/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          <  1:264703/140‑1 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          >  2:183833/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          >  2:170118/1‑140 (MQ=255)       gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGt                                                                                                                          <  1:877657/140‑1 (MQ=255)                  aCGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGt                                                                                                               <  1:966052/140‑1 (MQ=255)                     cTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAAc                                                                                                            >  1:516080/1‑140 (MQ=255)                        tttGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGtt                                                                                                         <  1:240332/140‑1 (MQ=255)                                gATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCa                                                                                                 <  1:16649/140‑1 (MQ=255)                                  ttAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACg                                                                                               >  1:1060958/1‑140 (MQ=255)                                  ttAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACg                                                                                               >  2:1048917/1‑140 (MQ=255)                                  ttAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACg                                                                                               >  1:122513/1‑140 (MQ=255)                                     aaaaaaaaTACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCt                                                                                            <  1:777230/140‑1 (MQ=255)                                             tACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCg                                                                                    <  1:459893/140‑1 (MQ=255)                                                 gTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATgc                                                                                <  2:1091809/140‑1 (MQ=255)                                                  tAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGTAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATgcg                                                                               <  1:582197/140‑1 (MQ=255)                                                  tAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATgcg                                                                               <  1:365231/140‑1 (MQ=255)                                                          gACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATa                                                                       >  2:828987/1‑140 (MQ=255)                                                                 ttCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGAcg                                                                <  1:48715/140‑1 (MQ=255)                                                                   ccccGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGAcgcg                                                              <  2:56966/140‑1 (MQ=255)                                                                                    gTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTgg                                             <  1:61564/140‑1 (MQ=255)                                                                                         gggAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTc                                        >  1:419446/1‑140 (MQ=255)                                                                                          ggAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCa                                       >  1:718889/1‑140 (MQ=255)                                                                                          ggAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCa                                       >  2:256663/1‑140 (MQ=255)                                                                                           gAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCAt                                      <  2:122513/140‑1 (MQ=255)                                                                                            aaCGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATg                                     <  1:1048917/140‑1 (MQ=255)                                                                                             aCGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGc                                    <  1:170118/140‑1 (MQ=255)                                                                                               gTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCg                                  >  1:799743/1‑140 (MQ=255)                                                                                                 aTTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTc                                <  2:419446/140‑1 (MQ=255)                                                                                                 aTTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTc                                <  1:87246/140‑1 (MQ=255)                                                                                                 aTTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTc                                >  1:423498/1‑140 (MQ=255)                                                                                                   ttAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCAt                              <  1:183833/140‑1 (MQ=255)                                                                                                   ttAATTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCAt                              <  2:271994/140‑1 (MQ=255)                                                                                                      aTTCCCCTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGga                           <  2:799743/140‑1 (MQ=255)                                                                                                          cccTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGgatgat                       >  1:54000/1‑140 (MQ=255)                                                                                                            cTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatca                     >  1:38765/1‑140 (MQ=255)                                                                                                            cTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatca                     >  1:499464/1‑140 (MQ=255)                                                                                                            cTGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatca                     >  1:134366/1‑140 (MQ=255)                                                                                                             tGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatcat                    >  1:268468/1‑140 (MQ=255)                                                                                                             tGCATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatcat                    <  2:718889/140‑1 (MQ=255)                                                                                                               cATCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatcatca                  >  2:179182/1‑140 (MQ=255)                                                                                                                aTCGCCCGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGatcatcat                 >  2:28601/1‑140 (MQ=255)                                                                                                                    cccGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATCcgc             <  2:268468/140‑1 (MQ=255)                                                                                                                     ccGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATcc               <  1:37175/137‑1 (MQ=255)                                                                                                                     ccGCATTCTTGCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATcc               >  2:37175/1‑137 (MQ=255)                                                                                                                               gCCGCACCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATCCGCGCCTTATGCAg  >  1:991630/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      CCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCCGTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTTTAAACTCACGGCTAAATTGCGATGCGCTTTCATAGCCGACGCGCATCGCTGCTGCGCTGGCCTTCATGCCGTCATGGATGATCATCATCCGCGCCTTATGCAG  >  NC_000913/3154164‑3154429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑