Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,167,049	T→C	T224A (ACT→GCT)	ygiS ←	putative deoxycholate binding periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,167,049	0	T	C	100.0%	313.6 / NA	83	T224A (ACT→GCT)	ygiS	putative deoxycholate binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/37);  total (46/37)

ACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCA  >  NC_000913/3166919‑3167183                                                                                                                                   |                                                                                                                                       aCCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTc                                                                                                                               <  1:1147711/140‑1 (MQ=255)   ccAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTTCTTTGGATTTTTGCGTGCAGCAATCTTTTCGt                                                                                                                             <  1:653302/140‑1 (MQ=255)   ccAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGt                                                                                                                             <  1:781280/140‑1 (MQ=255)    cAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGtt                                                                                                                            <  2:1149319/140‑1 (MQ=255)      ggTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTaa                                                                                                                          <  1:1104026/140‑1 (MQ=255)      ggTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTaa                                                                                                                          <  1:840192/140‑1 (MQ=255)      ggTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTaa                                                                                                                          >  1:9560/1‑140 (MQ=255)       gTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAAc                                                                                                                         <  1:1018865/140‑1 (MQ=255)       gTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAAc                                                                                                                         >  2:981848/1‑140 (MQ=255)         gagaTCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTa                                                                                                                       >  1:600102/1‑140 (MQ=255)            aTCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTAccc                                                                                                                    >  2:790018/1‑140 (MQ=255)            aTCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTAccc                                                                                                                    >  2:312414/1‑140 (MQ=255)             tCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCa                                                                                                                   <  2:451382/140‑1 (MQ=255)                aCCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCAc                                                                                                                  >  1:253794/1‑138 (MQ=255)                aCCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCAc                                                                                                                  <  2:253794/138‑1 (MQ=255)                  ctctCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGAt                                                                                                              <  2:952189/140‑1 (MQ=255)                    ctcCCACGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCa                                                                                                            <  2:1019041/140‑1 (MQ=255)                      cccGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAg                                                                                                          >  1:667137/1‑140 (MQ=255)                          cgcgATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGcaca                                                                                                      >  1:776558/1‑140 (MQ=255)                               tAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGc                                                                                                 >  1:718942/1‑140 (MQ=255)                                aGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCa                                                                                                <  2:600102/140‑1 (MQ=255)                                aGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCa                                                                                                <  1:956807/140‑1 (MQ=255)                                aGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCa                                                                                                <  2:667137/140‑1 (MQ=255)                                aGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCa                                                                                                <  2:871386/140‑1 (MQ=255)                                       ataGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGt                                                                                         >  2:410435/1‑140 (MQ=255)                                         aGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTaa                                                                                       >  2:250930/1‑140 (MQ=255)                                              gTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCAt                                                                                  <  2:395439/140‑1 (MQ=255)                                               tGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATg                                                                                 >  1:725437/1‑140 (MQ=255)                                               tGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATg                                                                                 >  2:68189/1‑140 (MQ=255)                                               tGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATg                                                                                 >  2:773986/1‑140 (MQ=255)                                                   cGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTtct                                                                             <  2:787051/140‑1 (MQ=255)                                                         aTCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCt                                                                       <  2:421336/140‑1 (MQ=255)                                                                  cAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGc                                                              <  2:776558/140‑1 (MQ=255)                                                                    gATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTa                                                            >  2:398699/1‑140 (MQ=255)                                                                        cTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGc                                                        >  2:281640/1‑140 (MQ=255)                                                                         tCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGcc                                                       <  1:312414/140‑1 (MQ=255)                                                                           aaCCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCa                                                      >  1:195066/1‑139 (MQ=255)                                                                           aaCCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCa                                                      <  2:195066/139‑1 (MQ=255)                                                                            aCCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGcc                                                       >  2:1063984/1‑137 (MQ=255)                                                                            aCCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGcc                                                       <  1:1063984/137‑1 (MQ=255)                                                                               tGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGcc                                                       >  1:959620/1‑134 (MQ=255)                                                                               tGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGcc                                                       <  2:959620/134‑1 (MQ=255)                                                                                 ttGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAg                                               >  1:1034408/1‑140 (MQ=255)                                                                                 ttGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAg                                               >  1:242309/1‑140 (MQ=255)                                                                                 ttGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAg                                               >  1:785238/1‑140 (MQ=255)                                                                                 ttGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAg                                               <  1:701130/140‑1 (MQ=255)                                                                                    cAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGa                                            <  1:981848/140‑1 (MQ=255)                                                                                       tACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGa                                         >  2:15019/1‑140 (MQ=255)                                                                                       tACTGTATGTAGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGa                                         <  1:790018/140‑1 (MQ=255)                                                                                        aCTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGAc                                        <  2:158639/140‑1 (MQ=255)                                                                                         cTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACa                                       <  2:725437/140‑1 (MQ=255)                                                                                           gTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatg                                     >  2:795389/1‑140 (MQ=255)                                                                                            tATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatga                                    >  2:967736/1‑140 (MQ=255)                                                                                            tATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatga                                    >  1:1107498/1‑140 (MQ=255)                                                                                            tATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatga                                    >  1:493106/1‑140 (MQ=255)                                                                                            tATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatga                                    >  1:751226/1‑140 (MQ=255)                                                                                            tATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACatga                                    >  2:51883/1‑140 (MQ=255)                                                                                                ttGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                <  2:718942/140‑1 (MQ=255)                                                                                                ttGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                <  2:785238/140‑1 (MQ=255)                                                                                                 tGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAgg                               >  2:949329/1‑140 (MQ=255)                                                                                                 tGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAgg                               >  1:865120/1‑140 (MQ=255)                                                                                                 tGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                >  2:1114214/1‑139 (MQ=255)                                                                                                 tGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                <  1:1114214/139‑1 (MQ=255)                                                                                                 tGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAACACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAgg                               >  1:328279/1‑140 (MQ=255)                                                                                                  gcgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGa                              >  2:547935/1‑140 (MQ=255)                                                                                                   cgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGaa                             >  2:294967/1‑140 (MQ=255)                                                                                                   cgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGaa                             >  1:1103213/1‑140 (MQ=255)                                                                                                   cgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGaa                             >  1:138764/1‑140 (MQ=255)                                                                                                   cgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGaa                             >  2:743435/1‑140 (MQ=255)                                                                                                   cgcATCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGaa                             >  1:761895/1‑140 (MQ=255)                                                                                                      aTCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                <  1:438154/134‑1 (MQ=255)                                                                                                      aTCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAg                                >  2:438154/1‑134 (MQ=255)                                                                                                      aTCGCGGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCg                          >  2:345272/1‑140 (MQ=255)                                                                                                          cgGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGaa                      <  2:865120/140‑1 (MQ=255)                                                                                                          cgGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGaa                      >  2:667963/1‑140 (MQ=255)                                                                                                          cgGTACTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGaa                      >  2:133181/1‑140 (MQ=255)                                                                                                               cTTTGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGCTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCg                 <  2:9560/140‑1 (MQ=255)                                                                                                                tttGGATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGt                >  2:947610/1‑140 (MQ=255)                                                                                                                    gATTTTTGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGc            <  1:410435/140‑1 (MQ=255)                                                                                                                       ttttGCGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAg         >  2:910951/1‑140 (MQ=255)                                                                                                                            cGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGa     <  1:921472/139‑1 (MQ=255)                                                                                                                            cGTGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGa     >  2:921472/1‑139 (MQ=255)                                                                                                                              tGCAGCAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCa  <  1:345272/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       ACCCAGGTGAGATCGACCTCTCCCGCGCGATAGCGGTTATAGCCGGTGACCGAATTATCTAGCGCCAGATACTCAACCTGTTGCAATACTGTATGTTGCGCATCGCGGTACTTTGGATTTTTGCGTGCAGTAATCTTTTCGTTAACTACCCACTGATCAAGCACAAAGGCACCGTTGTAAACCATGTTCTCTGGCTTACTCCAGCTATCGCCATGTTTAGCGATGACATGATGAGGAACCGGGAACAGCGTCGGCCAGGCGAGCA  >  NC_000913/3166919‑3167183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑