Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,215,455	C→T	A7T (GCT→ACT)	mug ←	stationary phase mismatch/uracil DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,215,455	0	C	T	100.0%	216.0 / NA	62	A7T (GCT→ACT)	mug	stationary phase mismatch/uracil DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/37);  total (25/37)

GGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTA  >  NC_000913/3215329‑3215583                                                                                                                               |                                                                                                                                 ggtcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCa                                                                                                                     <  2:461754/140‑1 (MQ=255)   tcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAAc                                                                                                                   <  1:901863/140‑1 (MQ=255)   tcggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAAc                                                                                                                   <  1:112496/140‑1 (MQ=255)    cggtAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATTCCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAAcc                                                                                                                  <  1:868189/140‑1 (MQ=255)    cggtAAACCCCGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCGCAGGGTTGATACCGCAAAACACGGCCCGTAACCCTGGAGTCAAAATATCCTCAAcc                                                                                                                  <  2:413479/140‑1 (MQ=255)         aaCCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATAtt                                                                                                             <  2:387373/140‑1 (MQ=255)            ccGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTAc                                                                                                          <  2:64265/140‑1 (MQ=255)             cGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACt                                                                                                         <  2:464828/140‑1 (MQ=255)             cGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACt                                                                                                         >  2:590797/1‑140 (MQ=255)             cGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACt                                                                                                         <  1:88110/140‑1 (MQ=255)              ggCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTc                                                                                                        <  1:860425/140‑1 (MQ=255)               gCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTcc                                                                                                       >  1:1032736/1‑140 (MQ=255)                       tatCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAAc                                                                                               <  1:309/140‑1 (MQ=255)                          cACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTAc                                                                                                          >  1:25143/1‑126 (MQ=255)                          cACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTAc                                                                                                          <  2:25143/126‑1 (MQ=255)                          cACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATc                                                                                            <  2:324674/140‑1 (MQ=255)                          cACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATc                                                                                            >  1:951818/1‑140 (MQ=255)                              ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                        >  1:184891/1‑140 (MQ=255)                              ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                        >  1:31046/1‑140 (MQ=255)                              ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                        >  2:769184/1‑140 (MQ=255)                              ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                        >  1:999197/1‑140 (MQ=255)                              ttCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCa                                                                                        >  1:980161/1‑140 (MQ=255)                               tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCAc                                                                                          <  2:588725/137‑1 (MQ=255)                               tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCAc                                                                                          >  1:588725/1‑137 (MQ=255)                               tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAg                                                                                       >  1:925321/1‑140 (MQ=255)                               tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAg                                                                                       >  2:936899/1‑140 (MQ=255)                               tCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAg                                                                                       >  1:761603/1‑140 (MQ=255)                                ccAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAgg                                                                                      <  2:49133/140‑1 (MQ=255)                                  aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                                    <  1:936899/140‑1 (MQ=255)                                  aGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                                    <  2:826713/140‑1 (MQ=255)                                    aaGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaaaa                                                                                  >  2:868559/1‑140 (MQ=255)                                      gCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                                    >  2:754169/1‑136 (MQ=255)                                      gCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGaa                                                                                    <  1:754169/136‑1 (MQ=255)                                             gCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACgg                                                                         <  1:810718/140‑1 (MQ=255)                                             gCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACgg                                                                         <  1:1026012/140‑1 (MQ=255)                                              ccGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGa                                                                        <  2:1098291/140‑1 (MQ=255)                                              ccGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGa                                                                        <  2:586201/140‑1 (MQ=255)                                               cGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGAt                                                                       <  1:96599/140‑1 (MQ=255)                                                        aaaGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATAc                                                              >  1:500235/1‑140 (MQ=255)                                                         aaGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACa                                                             <  2:627783/140‑1 (MQ=255)                                                            ggAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACAtta                                                          >  1:1027367/1‑139 (MQ=255)                                                            ggAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTg                                                          <  2:501623/140‑1 (MQ=255)                                                                 aCCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTtat                                                     <  2:46709/140‑1 (MQ=255)                                                                      tCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATaaaaa                                                >  1:405028/1‑140 (MQ=255)                                                                      tCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATaaaaa                                                >  1:594187/1‑140 (MQ=255)                                                                      tCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATaaaaa                                                >  1:847703/1‑140 (MQ=255)                                                                               aTGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcg                                       <  2:501398/140‑1 (MQ=255)                                                                               aTGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcg                                       <  1:590797/140‑1 (MQ=255)                                                                                tGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgc                                      <  2:980161/140‑1 (MQ=255)                                                                                tGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgc                                      <  2:1032736/140‑1 (MQ=255)                                                                                 gAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgcg                                     <  2:189198/140‑1 (MQ=255)                                                                                 gAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGgcgcg                                     <  2:500235/140‑1 (MQ=255)                                                                                     gCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGt                                 <  2:925321/140‑1 (MQ=255)                                                                                                aTACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGAt                      >  2:265697/1‑140 (MQ=255)                                                                                                aTACCGCAAAACACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGAt                      >  1:668921/1‑140 (MQ=255)                                                                                                       aaaaCACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGaca               >  1:943816/1‑140 (MQ=255)                                                                                                       aaaaCACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGaca               <  2:594187/140‑1 (MQ=255)                                                                                                       aaaaCACGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGaca               <  2:184891/140‑1 (MQ=255)                                                                                                          acacGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACgg            <  1:769184/140‑1 (MQ=255)                                                                                                            acGACCCGTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAg          <  2:665567/140‑1 (MQ=255)                                                                                                                   gTAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACttt   >  2:42175/1‑140 (MQ=255)                                                                                                                    tAACCCTGGAGTCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTta  >  2:123609/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                 GGTCGGTAAACCCGGCCTGATATATCACCTTCCAGAAGCGATTTGCCGGATGAGCAAAGGGAAAACCAGTCCCGGCGGATGAAAGCCCAGGGTTGATACCGCAAAACACGACCCGTAACCCTGGAGCCAAAATATCCTCAACCATATTTACTCCTTTCTAACATCACCAGGAAAAGTATAACGGATTGAAAATACATTGTTTATAAAAACAGCAGGCGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTA  >  NC_000913/3215329‑3215583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑