Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,405,411	T→C	intergenic (+953/‑25)	yhdH → / → accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,405,411	0	T	C	100.0%	276.7 / NA	74	intergenic (+953/‑25)	yhdH/accB	acrylyl‑CoA reductase/biotin carboxyl carrier protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/33);  total (41/33)

TGTCCGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGC  >  NC_000913/3405276‑3405539                                                                                                                                        |                                                                                                                                 tGTCCGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAAc                                                                                                                              >  1:158184/1‑140 (MQ=255) tGTCCGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAAc                                                                                                                              >  1:228090/1‑140 (MQ=255)  gTCCGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACa                                                                                                                             <  2:446578/140‑1 (MQ=255)    ccGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACcaaacaaa                                                                                                                           <  1:721090/140‑1 (MQ=255)      gTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAaga                                                                                                                         >  1:912127/1‑140 (MQ=255)      gTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAaga                                                                                                                         >  2:525959/1‑140 (MQ=255)               aaCATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAAc                                                                                                                <  2:939664/140‑1 (MQ=255)                aCATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAAcc                                                                                                               <  1:525959/140‑1 (MQ=255)                aCATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAAcc                                                                                                               <  2:912127/140‑1 (MQ=255)                aCATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAAcc                                                                                                               <  1:591059/140‑1 (MQ=255)                aCATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAAcc                                                                                                               <  2:416886/140‑1 (MQ=255)                          tctGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGaa                                                                                                                 >  2:699529/1‑128 (MQ=255)                          tctGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGaa                                                                                                                 <  1:699529/128‑1 (MQ=255)                          tctGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGa                                                                                                     >  1:729210/1‑140 (MQ=255)                           ctGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGat                                                                                                    <  1:1137480/140‑1 (MQ=255)                            tGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCAt                                                                                                        <  2:124026/135‑1 (MQ=255)                            tGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCAt                                                                                                        >  1:124026/1‑135 (MQ=255)                             ggggCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGatat                                                                                                  >  1:410356/1‑140 (MQ=255)                                gCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCg                                                                                               >  1:160603/1‑140 (MQ=255)                                      tAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGAt                                                                                         >  1:307068/1‑140 (MQ=255)                                        gACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTa                                                                                       >  2:154202/1‑140 (MQ=255)                                         aCCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTaa                                                                                      >  1:895441/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  2:428299/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  1:651891/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  1:268178/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  2:710330/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  2:1027256/1‑140 (MQ=255)                                              cGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAAc                                                                                 >  2:666485/1‑140 (MQ=255)                                                  ccGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGAt                                                                             >  1:304746/1‑140 (MQ=255)                                                     gCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGa                                                                          <  2:307068/140‑1 (MQ=255)                                                      ccTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAg                                                                         <  1:1042248/140‑1 (MQ=255)                                                      ccTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAg                                                                         <  2:410356/140‑1 (MQ=255)                                                      ccTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAg                                                                         <  2:277196/140‑1 (MQ=255)                                                      ccTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAg                                                                         <  2:651891/140‑1 (MQ=255)                                                        tgctgGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCt                                                                       >  1:889434/1‑140 (MQ=255)                                                         gctgGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTg                                                                      <  2:625676/140‑1 (MQ=255)                                                          ctgGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTgg                                                                     >  1:86807/1‑140 (MQ=255)                                                                    tCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATc                                                           <  2:160603/140‑1 (MQ=255)                                                                       cTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAgg                                                        <  2:895441/140‑1 (MQ=255)                                                                       cTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAgg                                                        <  1:628582/140‑1 (MQ=255)                                                                          tGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCAt                                                     >  1:761882/1‑140 (MQ=255)                                                                          tGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCAt                                                     >  1:677509/1‑140 (MQ=255)                                                                            cATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCAtct                                                   >  2:893206/1‑140 (MQ=255)                                                                                    tGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTg                                           >  1:80204/1‑140 (MQ=255)                                                                                    tGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTg                                           >  2:589188/1‑140 (MQ=255)                                                                                     gTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTgg                                          >  2:877874/1‑140 (MQ=255)                                                                                     gTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTgg                                          >  1:557808/1‑140 (MQ=255)                                                                                      tCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGa                                         >  2:376769/1‑140 (MQ=255)                                                                                      tCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGa                                         >  1:66528/1‑140 (MQ=255)                                                                                      tCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGa                                         >  2:175996/1‑140 (MQ=255)                                                                                       cGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGaa                                        >  1:24965/1‑140 (MQ=255)                                                                                                cGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGaa                               >  1:331077/1‑140 (MQ=255)                                                                                                    ttACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCg                           >  1:691936/1‑140 (MQ=255)                                                                                                    ttACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCg                           <  2:375518/140‑1 (MQ=255)                                                                                                    ttACAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCg                           <  1:175996/140‑1 (MQ=255)                                                                                                       cAGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAag                        <  2:268178/140‑1 (MQ=255)                                                                                                        aGACGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAaga                       <  2:80204/140‑1 (MQ=255)                                                                                                          aCGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGt                     <  2:66528/140‑1 (MQ=255)                                                                                                          aCGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGt                     <  1:877874/140‑1 (MQ=255)                                                                                                          aCGGCGGTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGt                     >  2:446649/1‑140 (MQ=255)                                                                                                           cggcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTc                    <  2:761882/140‑1 (MQ=255)                                                                                                           cggcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTc                    <  2:677509/140‑1 (MQ=255)                                                                                                            ggcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCa                   <  2:889434/140‑1 (MQ=255)                                                                                                            ggcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCa                   >  2:95563/1‑140 (MQ=255)                                                                                                             gcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAg                  <  2:691936/140‑1 (MQ=255)                                                                                                             gcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAg                  <  2:557808/140‑1 (MQ=255)                                                                                                             gcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAg                  >  1:1114492/1‑140 (MQ=255)                                                                                                             gcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAg                  >  1:1045521/1‑140 (MQ=255)                                                                                                             gcggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAg                  <  1:1027256/140‑1 (MQ=255)                                                                                                               ggTGAAACGCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTa                <  1:376769/140‑1 (MQ=255)                                                                                                                       gCCTGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAg        <  2:1114492/140‑1 (MQ=255)                                                                                                                        ccTGTCACAATCTCACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGc       >  2:102670/1‑140 (MQ=255)                                                                                                                          tGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCAt           >  2:1088663/1‑134 (MQ=255)                                                                                                                          tGTCACAATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCAt           <  1:1088663/134‑1 (MQ=255)                                                                                                                             cacaATCACACCAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGc  >  1:726821/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                 TGTCCGTCAAGTCTAACATTGACACTCTGGGGCAAAATAGACCGGCGTCCCGGCCTGCTGGAATTTATCGCTATGCATACAGCTGTCGGGGCATACGCTTTACAGACGGCGGTGAAACGCCTGTCACAATCACACTAAACAAAGAGTACGGAACCCACTCATGGATATTCGTAAGATTAAAAAACTGATCGAGCTGGTTGAAGAATCAGGCATCTCCGAACTGGAAATTTCTGAAGGCGAAGAGTCAGTACGCATTAGCCGTGC  >  NC_000913/3405276‑3405539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑