Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	305,455	A→G	F455L (TTC→CTC)	ecpD ←	fimbrial adhesin EcpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	305,455	0	A	G	100.0%	222.9 / NA	63	F455L (TTC→CTC)	ecpD	fimbrial adhesin EcpD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/35);  total (28/35)

ACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCTGGTGGTTACGATATAGCCG  >  NC_000913/305322‑305588                                                                                                                                      |                                                                                                                                      aCTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTAc                                                                                                                                 <  2:629990/140‑1 (MQ=255)             atatCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTAcc                                                                                                                     <  1:77453/140‑1 (MQ=255)                    tCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCg                                                                                                              <  2:51506/140‑1 (MQ=255)                    tCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCg                                                                                                              <  1:743225/140‑1 (MQ=255)                    tCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCg                                                                                                              >  1:208936/1‑140 (MQ=255)                     ccACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCgg                                                                                                             >  1:954453/1‑140 (MQ=255)                     ccACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCg                                                                                                              <  2:381218/139‑1 (MQ=255)                     ccACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCg                                                                                                              >  1:381218/1‑139 (MQ=255)                      cACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGa                                                                                                            >  1:773945/1‑140 (MQ=255)                      cACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGa                                                                                                            <  2:968455/140‑1 (MQ=255)                       aCGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAg                                                                                                           >  1:456468/1‑140 (MQ=255)                        cGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTAcc                                                                                                                     <  1:668761/129‑1 (MQ=255)                        cGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTAcc                                                                                                                     >  2:668761/1‑129 (MQ=255)                         ggTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCt                                                                                                         <  1:830711/140‑1 (MQ=255)                            gtgGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGaa                                                                                                      <  1:1029410/140‑1 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCt                                                                                                         <  1:277170/135‑1 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCt                                                                                                         >  2:277170/1‑135 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    <  1:776188/140‑1 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    <  2:566433/140‑1 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    <  2:360263/140‑1 (MQ=255)                              ggTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    <  2:54697/140‑1 (MQ=255)                               gTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGac                                                                                                   >  1:874616/1‑140 (MQ=255)                                     cacaGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATa                                                                                             >  1:861076/1‑140 (MQ=255)                                       caGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAgg                                                                                           >  2:397159/1‑140 (MQ=255)                                         gcgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGaa                                                                                                      <  2:404322/127‑1 (MQ=255)                                         gcgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGaa                                                                                                      >  1:404322/1‑127 (MQ=255)                                         gcgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    >  1:790162/1‑129 (MQ=255)                                         gcgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGa                                                                                                    <  2:790162/129‑1 (MQ=255)                                          cgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAgc                                                                                        >  1:614379/1‑140 (MQ=255)                                          cgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAgc                                                                                        >  1:16899/1‑140 (MQ=255)                                          cgcATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGACATCGGAGCTGAAGACACAATAGGAgc                                                                                        >  1:1145630/1‑140 (MQ=255)                                             aTCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGcgcc                                                                                     <  1:225576/140‑1 (MQ=255)                                              tCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGcgccc                                                                                    >  2:985281/1‑140 (MQ=255)                                                  tCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCa                                                                                <  1:985281/140‑1 (MQ=255)                                                  tCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCa                                                                                <  1:77314/140‑1 (MQ=255)                                                           gCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTgc                                                                       >  1:28084/1‑140 (MQ=255)                                                            ccAGCTATCATCGCACCCGGGATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTCCTTTCGCCTTCCCGTCATCGGGGCTGAAGACACAATAGGGGCGCCCGCCAATCACCTgcg                                                                      <  2:796701/140‑1 (MQ=255)                                                            ccAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTCCTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTgcg                                                                      <  2:658282/140‑1 (MQ=255)                                                            ccAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTgcg                                                                      >  1:1010672/1‑140 (MQ=255)                                                               gCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCgg                                                                   >  1:290296/1‑140 (MQ=255)                                                                  atcatcGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTc                                                                <  2:582889/140‑1 (MQ=255)                                                                        gCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCa                                                          <  2:874616/140‑1 (MQ=255)                                                                        gCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCa                                                          <  1:397159/140‑1 (MQ=255)                                                                         cACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCAc                                                         >  2:160676/1‑140 (MQ=255)                                                                          ccccGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCAcc                                                        <  1:160676/139‑1 (MQ=255)                                                                          ccccGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCAcc                                                        <  2:24190/139‑1 (MQ=255)                                                                          aCCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCAcc                                                        >  1:126939/1‑140 (MQ=255)                                                                          aCCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCAcc                                                        >  1:1121237/1‑140 (MQ=255)                                                                           cccGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATATGAGCGCCCGCCAATCACCTGCGCGCGTCCGGTCACCt                                                       >  1:459252/1‑140 (MQ=255)                                                                              ggCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGa                                                    >  1:733926/1‑140 (MQ=255)                                                                                             tGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCgg                                     >  1:1028634/1‑140 (MQ=255)                                                                                                        tGCGGGTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTa                          <  2:954453/140‑1 (MQ=255)                                                                                                           gggTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCg                       <  2:1028634/140‑1 (MQ=255)                                                                                                           gggTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCg                       <  2:1010672/140‑1 (MQ=255)                                                                                                             gTAATAAAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACTGCt                     <  2:861076/140‑1 (MQ=255)                                                                                                              taataaAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtg                    <  2:28084/140‑1 (MQ=255)                                                                                                               aataaAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtg                    >  1:912982/1‑139 (MQ=255)                                                                                                               aataaAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtg                    <  2:912982/139‑1 (MQ=255)                                                                                                                ataaAGGAAAGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtggt                  <  2:423128/140‑1 (MQ=255)                                                                                                                ataaAGGAAAGTGGCGCCGGGGGCGGTGCTTTCGGTTTCCCGTAACGGGCGGTGAAGACACAATAGGGGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtggt                  <  2:51189/140‑1 (MQ=255)                                                                                                                         aGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtg                    <  2:850358/129‑1 (MQ=255)                                                                                                                         aGTGTCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCtg                    >  1:850358/1‑129 (MQ=255)                                                                                                                           tgtCGCCGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCTGGTGGTTACGatat       >  1:616370/1‑140 (MQ=255)                                                                                                                                ccGGGAGCGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCTGGTGGTTACGATATAGCCg  <  2:1082897/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ACTTCGCCAGAGATATCAGTCCACGGTGTGGTCAACCACAGCGCATCGGTCATATCCCGCCAGCTATCATCGCACCCGGCATCGTAGGTTTTTGTAGCTCCGTTGCGGGTAATAAAGGAAAGTGTCGCCGGGAACGGTACTTTCGCCTTACCGTCATCGGAGCTGAAGACACAATAGGAGCGCCCGCCAATCACCTGCGCGGGTCCGGTCACCTTGATCAGCACTTCGTCGGCAGCTGTTTTACCGCTGGTGGTTACGATATAGCCG  >  NC_000913/305322‑305588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑