Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,752,077	T→C	V29A (GTA→GCA)	yiaU →	putative LysR‑type DNA‑binding transcriptional regulator YiaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,752,077	0	T	C	100.0%	175.6 / NA	48	V29A (GTA→GCA)	yiaU	putative LysR‑type DNA‑binding transcriptional regulator YiaU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/18);  total (30/18)

ATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACAT  >  NC_000913/3751959‑3752209                                                                                                                       |                                                                                                                                     atatatAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCaa                                                                                                                 >  2:532044/1‑140 (MQ=255)  tatataAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAAc                                                                                                                >  2:173359/1‑140 (MQ=255)    tataAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGt                                                                                                              >  2:902342/1‑140 (MQ=255)         aaTTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCa                                                                                                         >  1:90169/1‑140 (MQ=255)              tatataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAatat                                                                                                    >  2:1038448/1‑140 (MQ=255)                       gggCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAt                                                                                           >  1:474103/1‑140 (MQ=255)                        ggCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAtt                                                                                          >  1:1009910/1‑140 (MQ=255)                             ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                                                                     >  1:1041345/1‑140 (MQ=255)                             ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                                                                     >  2:1134705/1‑140 (MQ=255)                             ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                                                                     >  2:1039953/1‑140 (MQ=255)                             ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                                                                     >  1:92702/1‑140 (MQ=255)                             ttGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                                                                     >  1:924093/1‑140 (MQ=255)                                     aCAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                             >  2:975635/1‑140 (MQ=255)                                     aCAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGt                                                                             >  1:918708/1‑140 (MQ=255)                                       aaaaTTACAGCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTgg                                                                           >  1:329904/1‑140 (MQ=255)                                               aaCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaaa                                                                   >  1:365992/1‑140 (MQ=255)                                               aaCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaaa                                                                   >  1:1019648/1‑140 (MQ=255)                                                  ttAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTc                                                                <  1:1153783/140‑1 (MQ=255)                                                           gggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa                                                       >  2:808647/1‑140 (MQ=255)                                                            ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGaa                                                      >  2:828394/1‑140 (MQ=255)                                                            ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGaa                                                      >  1:918512/1‑140 (MQ=255)                                                                ttAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGTATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccac                                                  >  2:454958/1‑140 (MQ=255)                                                                ttAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccac                                                  >  1:50357/1‑140 (MQ=255)                                                                 tAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccacc                                                 >  1:203716/1‑140 (MQ=255)                                                                 tAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccacc                                                 >  2:408315/1‑140 (MQ=255)                                                                  aaaaaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCAccc                                                >  2:697417/1‑140 (MQ=255)                                                                               gTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCt                                   >  1:440392/1‑140 (MQ=255)                                                                                 aaTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTca                                 <  2:1019648/140‑1 (MQ=255)                                                                                 aaTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTca                                 <  2:783189/140‑1 (MQ=255)                                                                                   tCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                               <  1:975635/140‑1 (MQ=255)                                                                                   tCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                               <  1:828394/140‑1 (MQ=255)                                                                                   tCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTcaca                               <  1:697417/140‑1 (MQ=255)                                                                                          cAGTGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTg                        >  2:1015898/1‑140 (MQ=255)                                                                                             tGAAAATATCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCa                     >  2:363489/1‑140 (MQ=255)                                                                                                  atatCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACcgcg                <  1:886974/140‑1 (MQ=255)                                                                                                  atatCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACcgcg                <  2:918512/140‑1 (MQ=255)                                                                                                  atatCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACcgcg                <  1:173359/140‑1 (MQ=255)                                                                                                   tatCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCgcgc               <  1:532044/140‑1 (MQ=255)                                                                                                   tatCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCgcgc               <  1:39586/140‑1 (MQ=255)                                                                                                     tCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTg             <  2:50357/140‑1 (MQ=255)                                                                                                     tCAGCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTg             <  1:808647/140‑1 (MQ=255)                                                                                                        gCCATGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTa          <  2:329904/140‑1 (MQ=255)                                                                                                           aTGCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCCTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCg       <  1:432366/140‑1 (MQ=255)                                                                                                             gCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACcg                  >  2:348690/1‑127 (MQ=255)                                                                                                             gCCGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACcg                  <  1:348690/127‑1 (MQ=255)                                                                                                              ccGCGACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTAc    >  2:1028286/1‑140 (MQ=255)                                                                                                                gcgACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACAt  <  1:1015898/140‑1 (MQ=255)                                                                                                                gcgACTGCACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGAAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACAt  <  2:474103/140‑1 (MQ=255)                                                                                                                       |                                                                                                                                     ATATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGATGCTCACACCTTTTGGCACCGCGCTGTTACCGTACAT  >  NC_000913/3751959‑3752209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑