Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,946	T→C	T392A (ACC→GCC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,946	0	T	C	100.0%	88.4 / NA	27	T392A (ACC→GCC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/19);  total (8/19)

CAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACC  >  NC_000913/3878826‑3879076                                                                                                                         |                                                                                                                                    cAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGAcgc                                                                                                                  >  2:823091/1‑140 (MQ=255)  agagCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGAcgcg                                                                                                                 <  1:477797/140‑1 (MQ=255)   gagCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACgcgc                                                                                                                >  2:921858/1‑140 (MQ=255)          ccgcGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTca                                                                                                         <  2:399065/140‑1 (MQ=255)                            ccaGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTTCCGCGCGCGACGCGCCGCCTCACGGGCACGGGCAGCAtac                                                                                       >  2:563304/1‑138 (MQ=255)                             caGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGa                                                                                      <  2:98300/140‑1 (MQ=255)                                      ttCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGGCGCGCCGCCTCACCGGCACGGGGAGCATCGAT‑AAtttttc                                                                            >  2:730588/1‑138 (MQ=255)                                       tCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGcc                                                                           >  1:160279/1‑140 (MQ=255)                                        cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagc                                                                                           <  1:148183/124‑1 (MQ=255)                                        cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGgcagc                                                                                           >  2:148183/1‑124 (MQ=255)                                        cGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGGAGCATCGAT‑AATTTTTCCa                                                                          >  2:713219/1‑140 (MQ=255)                                           aaaGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAAcc                                                                       >  1:694965/1‑140 (MQ=255)                                                       cgcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTcgcg                                                           <  1:27321/140‑1 (MQ=255)                                                       cgcgTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTcgcg                                                           <  1:462855/140‑1 (MQ=255)                                                           ttCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCgg                                                       <  1:823091/140‑1 (MQ=255)                                                                    gTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCCTTTTACGCGCGCGACGCGCCGCTTCACCGGCCCGGGCAGCATCGATAAATTTTGCC‑ACCACCAATTTCGCGTCGGGTGGGgttt                                              >  2:833836/1‑140 (MQ=255)                                                                       tGCCAGTTTGCCCGGCAGG‑CCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTg                                                    <  1:512178/130‑1 (MQ=255)                                                                       tGCCAGTTTGCCCGGCAGG‑CCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTg                                                    >  2:512178/1‑130 (MQ=255)                                                                          cAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCcagca                                        <  1:921858/140‑1 (MQ=255)                                                                               tGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGGTGGGGTTTCCAGCAGGTAt                                   >  1:97316/1‑140 (MQ=255)                                                                                     gCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGcc                             <  1:730588/140‑1 (MQ=255)                                                                                         gCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCCTTTCCCCCGCCCGACGCGCCGCCTCCCGGGCACGGGGCGCATCGCT‑AAATTTTCCAACCACCATTTTCCCGTCGGGTGGGGTTTCCCGCCGGGATTCTTCcacac                         >  2:29126/1‑137 (MQ=255)                                                                                         gCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca                         <  2:160279/140‑1 (MQ=255)                                                                                         gCCCGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCcagca                         <  2:694965/140‑1 (MQ=255)                                                                                           ccGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgt                       <  1:104273/140‑1 (MQ=255)                                                                                            cGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt                      <  2:87155/140‑1 (MQ=255)                                                                                            cGCTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgtt                      <  1:181851/140‑1 (MQ=255)                                                                                              cTAAGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgttcg                    <  1:833836/140‑1 (MQ=255)                                                                                                 aGTCGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAgttcgttc                 <  1:563304/140‑1 (MQ=255)                                                                                                    cGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATc              <  1:824272/140‑1 (MQ=255)                                                                                                    cGAGCGCACCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATc              <  2:968670/140‑1 (MQ=255)                                                                                                           aCCTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTTCCAACCACGATTTTTGCGTCGGTTGGGGTTTTCCGCAGGGATTTTGCCAGCAGTTCGTTCATCTGCTGtt       >  2:403808/1‑140 (MQ=255)                                                                                                             cTTTACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTTCCAACCACGATTTTCGCGTCGGTTGGGGTTTTCAGCAGGGATTCTTCCAGCAGTTCGTTCATCTGCTGTTCa     >  2:824272/1‑140 (MQ=255)                                                                                                                tACGGCGGGCCATTTCACGCGCGCGACGCGCCGCTTCACGGGCCCGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTTCAGCAGGGATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAAcc  >  2:771560/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                    CAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCGAT‑AATTTTGCCAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACC  >  NC_000913/3878826‑3879076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑