Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,278	A→G	L319P (CTG→CCG)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,278	0	A	G	100.0%	299.1 / NA	80	L319P (CTG→CCG)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/43);  total (37/43)

CGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGAC  >  NC_000913/4232142‑4232414                                                                                                                                         |                                                                                                                                         cGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATgcggca                                                                                                                                       >  1:1142963/1‑140 (MQ=255)   tATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCata                                                                                                                                     >  2:752283/1‑140 (MQ=255)        ccAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAg                                                                                                                                <  1:756210/140‑1 (MQ=255)        ccAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAg                                                                                                                                <  1:48681/140‑1 (MQ=255)        ccAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAg                                                                                                                                <  2:248901/140‑1 (MQ=255)         cAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAgg                                                                                                                               <  1:405620/140‑1 (MQ=255)          aGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGc                                                                                                                              <  1:752283/140‑1 (MQ=255)            tGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCtg                                                                                                                            >  2:413768/1‑140 (MQ=255)             ggAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCtgt                                                                                                                           <  2:232455/140‑1 (MQ=255)              gAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCtgtg                                                                                                                          >  2:488090/1‑140 (MQ=255)               aGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGc                                                                                                                         <  2:1142963/140‑1 (MQ=255)                   tGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAg                                                                                                                     >  1:903914/1‑140 (MQ=255)                     aaCCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAgg                                                                                                                               <  1:832582/128‑1 (MQ=255)                     aaCCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAgg                                                                                                                               >  2:832582/1‑128 (MQ=255)                     aaCCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTg                                                                                                                   >  2:462442/1‑140 (MQ=255)                       ccGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAg                                                                                                                 <  2:915800/140‑1 (MQ=255)                       ccGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAg                                                                                                                 <  2:318890/140‑1 (MQ=255)                       ccGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAg                                                                                                                 <  2:847508/140‑1 (MQ=255)                         ggtggtATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCa                                                                                                               <  1:462442/140‑1 (MQ=255)                         ggtggtATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCa                                                                                                               <  2:695123/140‑1 (MQ=255)                                tCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTg                                                                                                        >  1:550979/1‑140 (MQ=255)                                tCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTg                                                                                                        >  1:634054/1‑140 (MQ=255)                                        tAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgac                                                                                                >  1:814316/1‑140 (MQ=255)                                         aaTGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgacg                                                                                               >  1:79891/1‑140 (MQ=255)                                          aTGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgacga                                                                                              >  1:722405/1‑140 (MQ=255)                                              cACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAgcg                                                                                          >  1:264070/1‑140 (MQ=255)                                               aCGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAgcgc                                                                                         <  2:264070/140‑1 (MQ=255)                                                cGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGcc                                                                                        >  1:645900/1‑140 (MQ=255)                                                cGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGcc                                                                                        >  2:646219/1‑140 (MQ=255)                                                 gCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCa                                                                                       <  2:645900/140‑1 (MQ=255)                                                 gCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCa                                                                                       <  1:107371/140‑1 (MQ=255)                                                    cACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGTCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgacg                                                                                               <  2:80943/129‑1 (MQ=255)                                                    cACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgacg                                                                                               >  1:80943/1‑129 (MQ=255)                                                           gACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGaa                                                                             <  1:245374/140‑1 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  1:687482/1‑140 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  2:566872/1‑140 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  1:649636/1‑140 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  1:486299/1‑140 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  2:1114687/1‑140 (MQ=255)                                                                 gtgATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgcgg                                                                       >  2:1089891/1‑140 (MQ=255)                                                                           cTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTc                                                              >  1:710880/1‑139 (MQ=255)                                                                           cTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTc                                                              <  2:710880/139‑1 (MQ=255)                                                                            tACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAg                                                            <  1:1114687/140‑1 (MQ=255)                                                                              ccGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAg                                                            <  2:923690/138‑1 (MQ=255)                                                                              ccGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAg                                                            >  1:923690/1‑138 (MQ=255)                                                                              ccGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGtt                                                          >  2:542650/1‑140 (MQ=255)                                                                               cGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGTAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGttt                                                         >  2:1096060/1‑140 (MQ=255)                                                                               cGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGttt                                                         <  2:79891/140‑1 (MQ=255)                                                                               cGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGttt                                                         <  1:542650/140‑1 (MQ=255)                                                                                   atatTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTAtt                                                     >  1:850950/1‑140 (MQ=255)                                                                                    tattatGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTg                                                    >  1:625281/1‑140 (MQ=255)                                                                                    tattatGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTg                                                    <  2:225409/140‑1 (MQ=255)                                                                                      ttatGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGca                                                  <  1:488672/140‑1 (MQ=255)                                                                                      ttatGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGca                                                  <  1:566872/140‑1 (MQ=255)                                                                                        atGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGcaca                                                >  2:762934/1‑140 (MQ=255)                                                                                         tGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCacac                                               >  2:31515/1‑140 (MQ=255)                                                                                         tGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCacac                                               <  1:501179/140‑1 (MQ=255)                                                                                         tGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCacac                                               <  1:762934/140‑1 (MQ=255)                                                                                         tGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCacac                                               <  2:625281/140‑1 (MQ=255)                                                                                          gCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACAcc                                              <  1:31515/140‑1 (MQ=255)                                                                                            cgcgcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAg                                            >  2:455495/1‑140 (MQ=255)                                                                                              cgcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCg                                          >  2:387098/1‑140 (MQ=255)                                                                                              cgcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCg                                          >  1:317712/1‑140 (MQ=255)                                                                                              cgcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCg                                          >  1:1078848/1‑140 (MQ=255)                                                                                               gcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGca                                                  <  2:981352/131‑1 (MQ=255)                                                                                               gcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGca                                                  >  1:981352/1‑131 (MQ=255)                                                                                               gcgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGt                                         >  1:195923/1‑140 (MQ=255)                                                                                                cgAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTa                                        >  2:699740/1‑140 (MQ=255)                                                                                                    gATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTaccac                                    <  1:1089891/140‑1 (MQ=255)                                                                                                    gATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTaccac                                    <  1:535199/140‑1 (MQ=255)                                                                                                      tGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCt                                  <  2:154203/140‑1 (MQ=255)                                                                                                           aaaaCTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGcgcg                             <  2:223083/140‑1 (MQ=255)                                                                                                           aaaaCTTCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGcgcg                             <  2:814316/140‑1 (MQ=255)                                                                                                                ttCCGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGt                        <  1:699740/140‑1 (MQ=255)                                                                                                                  ccGCGAGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCt                      >  2:849959/1‑140 (MQ=255)                                                                                                                       aGGAAACCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCt                 <  1:646219/140‑1 (MQ=255)                                                                                                                           aaCCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTCTTGCACACCAGCGTACCCCCTGCGCGTGGGTCTTTGCTGGAg             <  2:550979/140‑1 (MQ=255)                                                                                                                           aaCCGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAg             <  1:488090/140‑1 (MQ=255)                                                                                                                             ccGCGAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGcc           <  2:903914/140‑1 (MQ=255)                                                                                                                                cgAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGAc        <  2:634054/140‑1 (MQ=255)                                                                                                                                cgAGAATGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGAc        <  1:455495/140‑1 (MQ=255)                                                                                                                                      tGCGGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGAc  <  2:416103/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCACCTGCGCGTGGGTCTTTGCTGGAGCCGACAAAGAC  >  NC_000913/4232142‑4232414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑