Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,863	A→G	I465V (ATC→GTC)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,863	0	A	G	100.0%	190.3 / NA	53	I465V (ATC→GTC)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/27);  total (26/27)

GGCAATTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGATGCCGCCGCA  >  NC_000913/4276727‑4276999                                                                                                                                         |                                                                                                                                         ggCAATTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcgtca                                                                                                                                       >  2:118193/1‑140 (MQ=255) ggCAATTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACAcgtca                                                                                                                                       >  1:373105/1‑140 (MQ=255)    aaTTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCg                                                                                                                                    <  2:1040097/140‑1 (MQ=255)     aTTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGa                                                                                                                                   >  1:609146/1‑140 (MQ=255)         atgatTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTg                                                                                                                               <  1:693062/140‑1 (MQ=255)         atgatTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGAACACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTg                                                                                                                               <  1:58950/140‑1 (MQ=255)          tgatTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTgg                                                                                                                              >  1:331540/1‑140 (MQ=255)             ttttGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGgcgc                                                                                                                           <  2:34076/140‑1 (MQ=255)             ttttGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGgcgc                                                                                                                           <  2:705135/140‑1 (MQ=255)              tttGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGca                                                                                                                          <  1:729893/140‑1 (MQ=255)              tttGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGca                                                                                                                          <  1:285172/140‑1 (MQ=255)              tttGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGca                                                                                                                          <  2:1117053/140‑1 (MQ=255)                    aTTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCt                                                                                                                    <  2:609146/140‑1 (MQ=255)                            ctctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTaa                                                                                                            >  2:1002941/1‑140 (MQ=255)                            ctctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTaa                                                                                                            >  1:90482/1‑140 (MQ=255)                             tctAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTaaa                                                                                                           >  2:21881/1‑140 (MQ=255)                                aaCTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAAc                                                                                                        <  2:90482/140‑1 (MQ=255)                                      cATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGc                                                                                                  >  2:330998/1‑140 (MQ=255)                                           ccTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAg                                                                                             <  2:530264/140‑1 (MQ=255)                                           ccTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAg                                                                                             >  2:304824/1‑140 (MQ=255)                                           ccTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAg                                                                                             >  2:486809/1‑140 (MQ=255)                                                aaTCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTc                                                                                        >  2:931293/1‑140 (MQ=255)                                                aaTCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTc                                                                                        >  2:89379/1‑140 (MQ=255)                                                     ttGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAAc                                                                                   >  1:849069/1‑140 (MQ=255)                                                     ttGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAAc                                                                                   >  1:586408/1‑140 (MQ=255)                                                     ttGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAAc                                                                                   >  1:27027/1‑140 (MQ=255)                                                     ttGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAAc                                                                                   >  2:280115/1‑140 (MQ=255)                                                      tGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACTATCGCTGAAGGCGTCGAAACt                                                                                  >  1:971113/1‑140 (MQ=255)                                                      tGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACt                                                                                  >  1:688290/1‑140 (MQ=255)                                                      tGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCAACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACt                                                                                  >  2:187309/1‑140 (MQ=255)                                                          tGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTgagg                                                                              >  1:795767/1‑140 (MQ=255)                                                          tGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTgagg                                                                              <  1:330998/140‑1 (MQ=255)                                                          tGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGCCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTgagg                                                                              <  2:795767/140‑1 (MQ=255)                                                            tCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTgaggag                                                                            <  1:304824/140‑1 (MQ=255)                                                                   tttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                                                 <  1:115259/128‑1 (MQ=255)                                                                   tttGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTg                                                                                 >  2:115259/1‑128 (MQ=255)                                                                              aCAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTgcgc                                                          >  1:506340/1‑140 (MQ=255)                                                                                   tCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAcg                                                     <  2:688290/140‑1 (MQ=255)                                                                                     gTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACTATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAcgcg                                                   <  2:971113/140‑1 (MQ=255)                                                                                     gTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAcgcg                                                   <  1:1002941/140‑1 (MQ=255)                                                                                       ttgttgAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTTAGGAGCAGGTTAACTGGCTGCGCAAACgcggc                                                 >  1:853178/1‑140 (MQ=255)                                                                                        tgttgAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACgcggcg                                                <  2:265590/140‑1 (MQ=255)                                                                                         gttgAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGt                                               <  2:849069/140‑1 (MQ=255)                                                                                             aaaCGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAc                                                      <  1:61665/129‑1 (MQ=255)                                                                                             aaaCGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAc                                                      >  2:61665/1‑129 (MQ=255)                                                                                             aaaCGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTgcgc                                           >  2:535748/1‑140 (MQ=255)                                                                                              aaCGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCt                                          <  1:535748/140‑1 (MQ=255)                                                                                              aaCGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAAAGCGGCGTGCGCt                                          <  2:331540/140‑1 (MQ=255)                                                                                                    gACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc                                    <  1:21881/140‑1 (MQ=255)                                                                                                    gACCACCCACAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGcc                                    >  2:316564/1‑140 (MQ=255)                                                                                                         ccaacaAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCACTATTGCCAGGGa                               <  1:187309/137‑1 (MQ=255)                                                                                                          ccacAAAACCAGTCATTTGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGAt                              >  1:303424/1‑140 (MQ=255)                                                                                                                          ttGATTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGc              >  1:664519/1‑140 (MQ=255)                                                                                                                             aTTGCGGAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTTAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGAt           <  2:853178/140‑1 (MQ=255)                                                                                                                                  ggAACACGTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGATgccgc      <  1:280115/140‑1 (MQ=255)                                                                                                                                      cacgTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGATGCCGCCGCa  <  2:373105/140‑1 (MQ=255)                                                                                                                                      cacgTCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGATGCCGCCGCa  <  2:562192/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGCAATTGATGATTTTGGTATTGGCTACTCTAACTTGCATAACCTTAAATCATTGAATGTCGATATTTTGAAAATCGACAAATCGTTTGTTGAAACGCTGACCACCCACAAAACCAGTCATTTGATTGCGGAACACATCATCGAGCTGGCGCACAGCCTGGGGTTAAAAACGATCGCTGAAGGCGTCGAAACTGAGGAGCAGGTTAACTGGCTGCGCAAACGCGGCGTGCGCTATTGCCAGGGATGGTTCTTTGCGAAGGCGATGCCGCCGCA  >  NC_000913/4276727‑4276999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑