Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,256	(C)8→7	pseudogene (3831/5422 nt)	yneO ←	AIDA‑I family autotransporter YneO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,249	0	C	.	100.0%	103.6 / NA	25	pseudogene (3838/5422 nt)	yneO	AIDA‑I family autotransporter YneO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/15);  total (10/15)

TGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTG  >  NC_000913/1594123‑1594379                                                                                                                               |                                                                                                                                   tGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGatat                                                                                                                      >  2:292857/1‑140 (MQ=255) tGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGatat                                                                                                                      <  2:177729/140‑1 (MQ=255) tGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGatat                                                                                                                      <  1:86805/140‑1 (MQ=255)      cccccAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCcgtc                                                                                                                 >  1:228859/1‑140 (MQ=255)                           tCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                            <  1:156033/140‑1 (MQ=255)                                  cAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCgttgt                                                                                     <  1:374020/140‑1 (MQ=255)                                    gtggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTa                                                                                   <  2:297064/140‑1 (MQ=255)                                     tggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATCAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                            <  1:194450/130‑1 (MQ=255)                                     tggtgACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATCAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGaa                                                                                            >  2:194450/1‑130 (MQ=255)                                           cATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCgg                                                                                              >  1:120668/1‑122 (MQ=255)                                           cATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCgg                                                                                              <  2:120668/122‑1 (MQ=255)                                                          cacCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCa                                                             >  1:317297/1‑140 (MQ=255)                                                          cacCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCa                                                             >  1:180625/1‑140 (MQ=255)                                                                             aaTGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgct                                          <  2:261501/140‑1 (MQ=255)                                                                              aTGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCaccgcta                                         >  2:224744/1‑140 (MQ=255)                                                                                            cATATTAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACtgtg                           <  1:292857/140‑1 (MQ=255)                                                                                                 tAATGGCAGAGTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCa                      <  1:334717/140‑1 (MQ=255)                                                                                                          agTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGc             >  2:26293/1‑140 (MQ=255)                                                                                                           gTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt            <  2:317297/140‑1 (MQ=255)                                                                                                           gTCGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCt            <  1:224744/140‑1 (MQ=255)                                                                                                             cGGAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTAc          <  2:228859/140‑1 (MQ=255)                                                                                                              ggAACCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACg         <  1:26293/140‑1 (MQ=255)                                                                                                                 aCCGAAAGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCTGAATCGTTGTTAAGAGGCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTc      <  2:180625/140‑1 (MQ=255)                                                                                                                     aaaGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTg  >  2:392518/1‑140 (MQ=255)                                                                                                                      aaGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTg  <  2:11572/139‑1 (MQ=255)                                                                                                                      aaGCAATTT‑CCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTg  >  1:11572/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                   TGCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCCCACGATATCCGTCGTACCCTGATACGCCGCGGAATCGTTGTTAAGAGTCAGGATCCCGGTACCCAGTTTTGTCACATCACCGCTACCGCTGATACTGTGCGTCATCGCCCAGCTACGCTCAGTG  >  NC_000913/1594123‑1594379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑