Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,135,663	(G)5→6	coding (100/699 nt)	flgH →	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,135,658	1	.	G	100.0%	88.2 / NA	25	Q32R (CAG→CGG)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (16/9);  total (16/9)

GGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCA‑GGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTGGCG  >  NC_000913/1135528‑1135789                                                                                                                                    |                                                                                                                                    ggTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCA‑ggggggcga                                                                                                                            >  1:119909/1‑136 (MQ=255)   ttCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGAcc                                                                                                                           >  2:226277/1‑140 (MQ=255)             tACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAAc                                                                                                                 >  1:7537/1‑140 (MQ=255)                                      tGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTc                                                                                                     <  2:68015/127‑1 (MQ=255)                                      tGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTc                                                                                                     >  1:68015/1‑127 (MQ=255)                                       gCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGcc                                                                                       >  1:17216/1‑140 (MQ=255)                                            aaaaCGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACgg                                                                                  <  2:189034/140‑1 (MQ=255)                                                 gCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTa                                                                             >  2:197752/1‑140 (MQ=255)                                                     cgcATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTAtttt                                                                         <  2:119909/140‑1 (MQ=255)                                                          aCTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGt                                                                    <  2:7537/140‑1 (MQ=255)                                                                   aTTTCAAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTAtttt                                                                         <  1:320794/126‑1 (MQ=255)                                                                   aTTTCAAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTAtttt                                                                         >  2:320794/1‑126 (MQ=255)                                                                    tttCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGcc                                                          >  2:82593/1‑140 (MQ=255)                                                                           cTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAAc                                                   >  2:295969/1‑140 (MQ=255)                                                                                        tCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAAc                                      >  2:222813/1‑140 (MQ=255)                                                                                             aaCCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTg                                 >  2:344996/1‑140 (MQ=255)                                                                                              aCCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGt                                <  1:344996/140‑1 (MQ=255)                                                                                                   cTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGaa                           <  1:226277/140‑1 (MQ=255)                                                                                                            gATACCCTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGa                  <  1:295969/140‑1 (MQ=255)                                                                                                                 ccTCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACg             >  2:228253/1‑140 (MQ=255)                                                                                                                   tCCACGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCa           >  2:139037/1‑140 (MQ=255)                                                                                                                      aCGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAata        >  2:290924/1‑140 (MQ=255)                                                                                                                      aCGCCGCTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAata        >  1:77202/1‑140 (MQ=255)                                                                                                                          cgcTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTgg    >  1:71362/1‑140 (MQ=255)                                                                                                                           gcTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTGGc   >  2:345426/1‑140 (MQ=255)                                                                                                                            cTGGTGCAGGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTGGCg  <  1:222813/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    GGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCA‑GGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTATGGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTGGCG  >  NC_000913/1135528‑1135789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑