Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,879,362 (T)8→9 intergenic (‑107/+41) yeaQ ← / ← yoaG PF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,879,3541.T93.8% 103.5 / 0.2 32intergenic (‑99/+49)yeaQ/yoaGPF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base T (16/14);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

CAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCA  >  NC_000913/1879228‑1879485
                                                                                                                               |                                                                                                                                    
cAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑ttt‑ttttttgcga                                                                                                                        <  1:238135/140‑6 (MQ=255)
             tGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAAtt                                                                                                            >  1:247152/1‑140 (MQ=255)
             tGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAAtt                                                                                                            >  2:33934/1‑140 (MQ=255)
                cAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTT‑TTTTTGCGATGGCGCAGAAATTgcgc                                                                                                        <  2:364706/140‑1 (MQ=255)
                      gCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCa                                                                                                   <  2:391706/140‑1 (MQ=255)
                       cccATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCaa                                                                                                  <  1:33934/140‑1 (MQ=255)
                         cATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACg                                                                                                <  1:58571/140‑1 (MQ=255)
                             gTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCa                                                                                            <  1:375843/140‑1 (MQ=255)
                               ttCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGt                                                                                          <  2:82199/140‑1 (MQ=255)
                                     aCTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAAt                                                                                    >  2:361073/1‑140 (MQ=255)
                                          tCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTacca                                                                               >  1:355535/1‑140 (MQ=255)
                                                        gCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAcatcat                                                                 >  2:88930/1‑140 (MQ=255)
                                                        gCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAcatcat                                                                 >  2:87577/1‑140 (MQ=255)
                                                                   gCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTcc                                                      <  2:65233/140‑1 (MQ=255)
                                                                    cTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCg                                                     <  1:87577/140‑1 (MQ=255)
                                                                                 tAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACg                                        >  1:116428/1‑140 (MQ=255)
                                                                                   gCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTa                                      <  2:101785/140‑1 (MQ=255)
                                                                                   gCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTa                                      <  2:247152/140‑1 (MQ=255)
                                                                                   gCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTa                                      <  1:361073/140‑1 (MQ=255)
                                                                                     acaacaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACg                                    <  1:88930/140‑1 (MQ=255)
                                                                                     acaacaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTTATTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACg                                    <  2:200335/140‑1 (MQ=255)
                                                                                      caacaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACgg                                   <  2:116428/140‑1 (MQ=255)
                                                                                         caTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTAt                                >  2:226644/1‑140 (MQ=255)
                                                                                            cGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCa                             >  1:344833/1‑140 (MQ=255)
                                                                                            cGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCa                             >  1:367666/1‑140 (MQ=255)
                                                                                              ggAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCAcc                           >  1:108302/1‑140 (MQ=255)
                                                                                                   gCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATc                      >  2:4019/1‑140 (MQ=255)
                                                                                                   gCAAGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATc                      >  1:4534/1‑140 (MQ=255)
                                                                                                     aaGATTTGTGACGAGCATCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCtt                    >  1:391776/1‑140 (MQ=255)
                                                                                                                     aTCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAgccgc    <  1:226644/140‑1 (MQ=255)
                                                                                                                      tCACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAgccgcc   <  2:344833/140‑1 (MQ=255)
                                                                                                                       cACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCa  >  1:189650/1‑140 (MQ=255)
                                                                                                                       cACGGAGGTTTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCa  >  2:323922/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                    
CAAAAATAATCCATGACAGAATGCCCATTGTTTCCTCACTTATCTGTTTTGCATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTT‑TTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTTTTCCGTGTCATAAGAACGTACGGTATTCACCAGATCTTTTATCACTTCAGCCGCCA  >  NC_000913/1879228‑1879485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑