| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,167,837 | (C)5→6 | intergenic (‑119/+14) | ygiS ← / ← mqsA | putative deoxycholate binding periplasmic protein/antitoxin of the MqsRA toxin‑antitoxin system/DNA‑binding transcriptional repressor MqsA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,167,832 | 1 | . | C | 95.5% | 67.4 / ‑2.9 | 22 | intergenic (‑114/+19) | ygiS/mqsA | putative deoxycholate binding periplasmic protein/antitoxin of the MqsRA toxin‑antitoxin system/DNA‑binding transcriptional repressor MqsA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (9/12); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
ATAAATTTCGCGTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTG‑CCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTGGAAGGATGAGGTTGGGCATTGCCTTTTTCGTAACGCGAAAACGCATTTACAC > NC_000913/3167700‑3167963 | ataaATTTCGCGTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTG‑CCCCCGc > 2:209406/1‑140 (MQ=255) tttCGCGTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCtttt > 1:283979/1‑140 (MQ=255) cgcgTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTGGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCa > 2:359552/1‑140 (MQ=255) cgcgTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCa < 1:59942/140‑1 (MQ=255) cgcgTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCa < 1:80272/140‑1 (MQ=255) aTTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGAtt < 2:382687/140‑1 (MQ=255) cTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATttcat > 1:226086/1‑140 (MQ=255) aCCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCattaa > 1:113975/1‑123 (MQ=255) aCCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCattaa < 2:113975/123‑1 (MQ=255) aTTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTg < 1:388994/140‑1 (MQ=255) tataAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGa < 1:144335/140‑1 (MQ=255) aGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCt < 2:108385/140‑1 (MQ=255) gCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCtt < 1:149925/140‑1 (MQ=255) cGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGTTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTa > 1:64931/1‑140 (MQ=255) cTGTGGCATTGTTCGCTCAAACTTATCGCGAGGGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTaaaa > 1:356753/1‑140 (MQ=255) cATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGa < 2:226086/140‑1 (MQ=255) gTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATtgt < 2:10058/140‑1 (MQ=255) tCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTgg > 1:51087/1‑136 (MQ=255) tCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTgg < 2:51087/136‑1 (MQ=255) cTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTGGAAGGATGAg > 1:317598/1‑140 (MQ=255) acTCCGGTAAAGAAAAAAGCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTGGAAGGATGAGGTTGGGCATTGCCTTTTTCGTAAc > 2:43152/1‑140 (MQ=255) gCGAGTTGCCCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTGGAAGGATGAGGTTGGGCATTGCCTTTTTCGTAACGCGAAAACGCATTTacac < 2:283979/140‑1 (MQ=255) | ATAAATTTCGCGTATACATGGTATTCCTTACCGGAGAAATTATAAGCCCAACAACAATACGCCTGTGGCATTGTTCGCTCAAACTTATCGCGAGTGATTTGGCTCACACTCCGGTAAAGAAAAAAGCGAGTTG‑CCCCCGCTTTTCCATTAATTAACGGATTTCATTCAATAGTTCTGGATGCTTATCCAGAACACGTAAAAGTTTGATTGTGGAAGGATGAGGTTGGGCATTGCCTTTTTCGTAACGCGAAAACGCATTTACAC > NC_000913/3167700‑3167963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |