Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	57,230	A→G	intergenic (‑121/‑134)	lptD ← / → djlA	lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	57,230	0	A	G	100.0%	83.5 / NA	24	intergenic (‑121/‑134)	lptD/djlA	lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

CGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGG  >  NC_000913/57099‑57356                                                                                                                                    |                                                                                                                               cGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTg                                                                                                                        >  2:283451/1‑140 (MQ=255)   ttttttCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGaa                                                                                                                      >  2:30357/1‑140 (MQ=255)     ttttCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATg                                                                                                                    <  1:122139/140‑1 (MQ=255)     ttttCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATg                                                                                                                    >  2:237896/1‑140 (MQ=255)            aCGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATg                                                                                                                    >  2:286846/1‑133 (MQ=255)            aCGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATg                                                                                                                    <  1:286846/133‑1 (MQ=255)                tGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGAt                                                                                                         <  2:14576/140‑1 (MQ=255)                      tATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCa                                                                                                   <  1:81931/140‑1 (MQ=255)                          gTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAAtt                                                                                               >  2:124621/1‑140 (MQ=255)                            tCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATtat                                                                                             >  1:118631/1‑140 (MQ=255)                                 catcGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCg                                                                                        <  1:168699/140‑1 (MQ=255)                                          cGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCcac                                                                               >  2:262306/1‑140 (MQ=255)                                                 cgTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGAc                                                                        <  2:53228/140‑1 (MQ=255)                                                                       aCGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAg                                                  <  2:118631/140‑1 (MQ=255)                                                                       aCGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAg                                                  <  1:369741/140‑1 (MQ=255)                                                                                           cTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGgaga                              <  1:262306/140‑1 (MQ=255)                                                                                           cTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGgaga                              <  1:124621/140‑1 (MQ=255)                                                                                               tgtTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGc                          <  1:30357/140‑1 (MQ=255)                                                                                                 ttAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGc                          >  2:12716/1‑138 (MQ=255)                                                                                                 ttAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGc                          <  1:12716/138‑1 (MQ=255)                                                                                                   aaTCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTg                      >  1:36906/1‑140 (MQ=255)                                                                                                   aaTCCTGTCCGAATAGCGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTg                      >  1:170995/1‑140 (MQ=255)                                                                                                                   cGTCAGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTACATTGTGGGAGATAGCCCTGATATCCGTGTGTCGAt      >  2:284906/1‑140 (MQ=255)                                                                                                                       aGTGGTGTTAGGCGCGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTgg  <  2:170995/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                               CGTTTTTTCATACGTTGATTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGG  >  NC_000913/57099‑57356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑