Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,179,724	A→G	P43P (CCA→CCG)	ygiA →	putative protein YgiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,179,724	0	A	G	100.0%	73.2 / NA	21	P43P (CCA→CCG)	ygiA	putative protein YgiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/7);  total (14/7)

ATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAG  >  NC_000913/3179596‑3179860                                                                                                                                 |                                                                                                                                         atgaCGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGa                                                                                                                               >  1:375415/1‑140 (MQ=255)       acgacgGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACaa                                                                                                                         >  2:384375/1‑140 (MQ=255)                     gCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACa                                                                                                           >  2:257641/1‑140 (MQ=255)                              gAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATa                                                                                                  <  2:375415/140‑1 (MQ=255)                                    aaGGCAACGTAAAGATACGGGTTATCTGCCGCATACTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAACATGAAACGGACAAAATCCATACGCCAc                                                                                            >  2:350770/1‑140 (MQ=255)                                                     cGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCaaaaa                                                                           >  1:46751/1‑140 (MQ=255)                                                            tCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAgc                                                                    >  2:354454/1‑140 (MQ=255)                                                                                           tttCGACCAGCCATCCTCTATTCTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCgg                                     >  2:377114/1‑140 (MQ=255)                                                                                                                 cTGATGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggc               <  1:384375/140‑1 (MQ=255)                                                                                                                    aTGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  2:379307/1‑140 (MQ=255)                                                                                                                    aTGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  2:167018/1‑140 (MQ=255)                                                                                                                    aTGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  2:354092/1‑140 (MQ=255)                                                                                                                    aTGGGTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  2:303102/1‑140 (MQ=255)                                                                                                                    aTGGGGATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  1:298792/1‑140 (MQ=255)                                                                                                                       ggTATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCACAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctggctg         >  2:194684/1‑140 (MQ=255)                                                                                                                         tATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            >  1:80290/1‑135 (MQ=255)                                                                                                                         tATTTACCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATgctggctgg            <  2:80290/135‑1 (MQ=255)                                                                                                                              aCCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag  <  2:298792/140‑1 (MQ=255)                                                                                                                              aCCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag  <  1:354092/140‑1 (MQ=255)                                                                                                                              aCCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag  <  1:303102/140‑1 (MQ=255)                                                                                                                              aCCGCTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAag  <  1:167018/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         ATGACGACGACGGGGCTTCGGCCCCGTCTGAACGTAAGGCAACGTAAAGATACGGGTTATCTGCCGCATTCTTCCCCCTTCTCGCTTCAATTTCGACCAGCCATCCTCTATTCTGATGGGTATTTACCACTGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAG  >  NC_000913/3179596‑3179860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑