Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,696	G→A	A225T (GCA→ACA)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,696	0	G	A	100.0%	46.7 / NA	15	A225T (GCA→ACA)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

ACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGT  >  NC_000913/3208571‑3208826                                                                                                                              |                                                                                                                                   aCAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCTACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTcacgccacg                                                                                                                      <  2:344504/140‑1 (MQ=255)        ttCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGtt                                                                                                               >  1:176499/1‑140 (MQ=255)                     ggCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGtt                                                                                                  <  2:176499/140‑1 (MQ=255)                           tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTTGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCgg                                                                                            >  2:311229/1‑140 (MQ=255)                                catGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGt                                                                                                   >  1:310057/1‑128 (MQ=255)                                catGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGt                                                                                                   <  2:310057/128‑1 (MQ=255)                                        tGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc                                                                               >  1:250445/1‑140 (MQ=255)                                        tGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTc                                                                               >  2:181323/1‑140 (MQ=255)                                            gATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCa                                                                           >  1:256130/1‑140 (MQ=255)                                                 ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTa                                                                      >  1:395975/1‑140 (MQ=255)                                                            gTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct                                                           <  1:181323/140‑1 (MQ=255)                                                            gTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct                                                           <  1:356918/140‑1 (MQ=255)                                                                   gcgcCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTccc                                                    >  2:137388/1‑140 (MQ=255)                                                                             cTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCtt                                          <  1:137388/140‑1 (MQ=255)                                                                                                                     atgaAAAGCACATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGt  >  1:42755/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                   ACAGCAGTTCGCGCAGCATTGGCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGT  >  NC_000913/3208571‑3208826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑