Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,612	T→C	S79G (AGC→GGC)	yraR ←	putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,612	0	T	C	100.0%	88.1 / NA	25	S79G (AGC→GGC)	yraR	putative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

GGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAA  >  NC_000913/3298491‑3298735                                                                                                                          |                                                                                                                            ggCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCgt                                                                                                           <  2:373781/140‑1 (MQ=255)                              gACTACCAACATATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGa                                                                              <  2:249391/140‑1 (MQ=255)                                         tatGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGACGATGGGATCGGTg                                                                   >  2:244032/1‑140 (MQ=255)                                           tgctgcGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGAc                                                                 >  1:239088/1‑140 (MQ=255)                                             ctgcGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCt                                                               >  2:74984/1‑140 (MQ=255)                                                 gcgcCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTgcgc                                                           >  1:296670/1‑140 (MQ=255)                                                   gcCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCa                                                         <  1:114750/140‑1 (MQ=255)                                                     ccAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAAc                                                       >  1:71528/1‑140 (MQ=255)                                                             cgccCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACt                                               <  2:323042/140‑1 (MQ=255)                                                                   gTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTg                                         >  2:270399/1‑140 (MQ=255)                                                                               tCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATgggg                             <  1:74984/140‑1 (MQ=255)                                                                                        aGCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAacac                    <  1:270399/140‑1 (MQ=255)                                                                                         gCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATggg                              <  2:14118/129‑1 (MQ=255)                                                                                         gCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATggg                              >  1:14118/1‑129 (MQ=255)                                                                                         gCGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACAcc                   >  2:201515/1‑140 (MQ=255)                                                                                          cGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCAt                                 >  2:270272/1‑125 (MQ=255)                                                                                          cGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCAt                                 <  1:270272/125‑1 (MQ=255)                                                                                          cGTGTAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACAccc                  >  2:393056/1‑140 (MQ=255)                                                                                            tgtAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCg                >  1:395235/1‑140 (MQ=255)                                                                                             gtAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCgg               <  2:296670/140‑1 (MQ=255)                                                                                             gtAATCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCgg               <  2:71528/140‑1 (MQ=255)                                                                                                aTCGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCa            >  2:290873/1‑140 (MQ=255)                                                                                                  cGGCATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCata          <  1:290873/140‑1 (MQ=255)                                                                                                     cATGAATAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCg       >  1:36600/1‑140 (MQ=255)                                                                                                          aTAAACGCCTCTTTGCCCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCaa  >  1:57927/1‑140 (MQ=255)                                                                                                                          |                                                                                                                            GGCGAGTGGGCATTGGCACCCATCGCACTGACTACCAACATATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAA  >  NC_000913/3298491‑3298735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑