| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,318,699 | T→C | G21G (GGT→GGC) | argG → | argininosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,318,699 | 0 | T | C | 96.0% | 79.8 / ‑3.9 | 25 | G21G (GGT→GGC) | argG | argininosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (10/14); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
TGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCG > NC_000913/3318570‑3318829 | tGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCa > 1:153676/1‑140 (MQ=255) aaTTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCa < 2:260377/140‑1 (MQ=255) atcaGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTc < 2:144186/140‑1 (MQ=255) caGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCt < 1:381860/140‑1 (MQ=255) caGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCt < 2:75343/140‑1 (MQ=255) aattaaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGc > 1:203610/1‑140 (MQ=255) aattaaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGc > 1:256881/1‑140 (MQ=255) aattaaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGc > 1:364016/1‑140 (MQ=255) aattaaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGc < 2:324019/140‑1 (MQ=255) taaGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCata < 1:97313/140‑1 (MQ=255) aGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCAtata > 2:333900/1‑140 (MQ=255) cAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACt > 2:193448/1‑140 (MQ=255) gtTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAAc < 1:333900/140‑1 (MQ=255) ttATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGgc < 1:278607/140‑1 (MQ=255) aTCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTat < 1:390660/140‑1 (MQ=255) cccGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTatgatg < 2:153676/140‑1 (MQ=255) tAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATc < 2:203610/140‑1 (MQ=255) aaCGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCtcgt > 1:86943/1‑140 (MQ=255) aCGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCtcgtc < 2:86943/140‑1 (MQ=255) aCGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCtcgtc < 2:256881/140‑1 (MQ=255) cGTATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCtcgtcg > 1:36266/1‑140 (MQ=255) tATTGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTg < 2:36266/140‑1 (MQ=255) tGGTATCGCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCa > 1:203462/1‑140 (MQ=255) gCTTTTTCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATa > 1:171270/1‑140 (MQ=255) ttCTGGCGGCCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGgcg < 2:171270/140‑1 (MQ=255) | TGCATAAAAATTCAGTGAGAGCGGAAATCCAGGCTCATCATCAGTTAATTAAGCAGGGTGTTATTTTATGACGACGATTCTCAAGCATCTCCCGGTAGGTCAACGTATTGGTATCGCTTTTTCTGGCGGTCTGGACACCAGTGCCGCACTGCTGTGGATGCGACAAAAGGGAGCGGTTCCTTATGCATATACTGCAAACCTGGGCCAGCCAGACGAAGAGGATTATGATGCGATCCCTCGTCGTGCCATGGAATACGGCG > NC_000913/3318570‑3318829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |