Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,326,663	T→C	E91E (GAA→GAG)	ftsH ←	ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,326,663	0	T	C	100.0%	105.7 / NA	30	E91E (GAA→GAG)	ftsH	ATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/11);  total (19/11)

ACCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCG  >  NC_000913/3326531‑3326790                                                                                                                                     |                                                                                                                                aCCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGa                                                                                                                          <  2:175796/140‑1 (MQ=255)    aaaCGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACaa                                                                                                                       >  2:263983/1‑140 (MQ=255)                aCCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCt                                                                                                           >  1:16616/1‑140 (MQ=255)                            gccgccCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGt                                                                                               >  2:67435/1‑140 (MQ=255)                                     cATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTa                                                                                      >  2:60538/1‑140 (MQ=255)                                        ttGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAAtt                                                                                   >  1:9285/1‑140 (MQ=255)                                               aTGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATc                                                                            >  1:278794/1‑140 (MQ=255)                                                           cAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAAt                                                                <  1:263983/140‑1 (MQ=255)                                                               cacCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTaa                                                            >  1:127366/1‑140 (MQ=255)                                                                   aaTCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGTATGTAAGTgg                                                        >  2:297137/1‑140 (MQ=255)                                                                    aTCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGTATGTAAGTGGt                                                       <  1:297137/140‑1 (MQ=255)                                                                    aTCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGt                                                       >  1:316321/1‑140 (MQ=255)                                                                      cAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGtat                                                     <  2:9285/140‑1 (MQ=255)                                                                       aGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGtata                                                    <  1:60538/140‑1 (MQ=255)                                                                       aGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGtata                                                    >  1:227102/1‑140 (MQ=255)                                                                       aGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGtata                                                    >  1:201080/1‑140 (MQ=255)                                                                        gCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATaa                                                   <  2:227102/140‑1 (MQ=255)                                                                              gCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTa                                             >  2:42906/1‑140 (MQ=255)                                                                               cATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGt                                               <  1:365810/137‑1 (MQ=255)                                                                               cATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGt                                               >  2:365810/1‑137 (MQ=255)                                                                               cATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTAc                                            >  1:352048/1‑140 (MQ=255)                                                                               cATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTAc                                            >  2:81614/1‑140 (MQ=255)                                                                                 tCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTa                                          <  2:201080/140‑1 (MQ=255)                                                                                 tCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTa                                          >  2:346553/1‑140 (MQ=255)                                                                                                           gCCAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACg                <  1:346553/140‑1 (MQ=255)                                                                                                            ccAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGt               <  1:81614/140‑1 (MQ=255)                                                                                                            ccAGCAGGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGt               >  1:14862/1‑140 (MQ=255)                                                                                                               gcagGCTTGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCg            >  1:258839/1‑140 (MQ=255)                                                                                                                     ttGGTTCTTCAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATa      >  1:63676/1‑140 (MQ=255)                                                                                                                         ttcttcAGGCGGCTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATAcgcg  <  2:258839/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                ACCAAACGACATGGCACCTTTGCCACCGCCGCCCTGCATTTGACGCATGAAGAAGATCCAGACACCAATCAGCAACAGCATCGGGAACCAGGAGATGAAGATAGAAGCCAGCAGGCTTGGTTCTTCAGGCGGTTCACCGACAACCTTGACGTTCTTGGTCAACAGGTTATCCAGTAATTTCGGATCCTGAACCGGAATGTAAGTGGTATAACGGTTACTATCTTTCTTGGTAACGTTGATTTCACGTCCGTTGATACGCG  >  NC_000913/3326531‑3326790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑