Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,708	T→C	A199A (GCT→GCC)	yhcA →	putative fimbrial chaperone YhcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,708	0	T	C	100.0%	126.9 / NA	36	A199A (GCT→GCC)	yhcA	putative fimbrial chaperone YhcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

AGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCTACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTGGCC  >  NC_000913/3362589‑3362830                                                                                                                        |                                                                                                                           aGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGtt                                                                                                        >  2:70924/1‑140 (MQ=255)       tATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTAccc                                                                                                  <  2:327496/140‑1 (MQ=255)          gTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACccagc                                                                                               >  1:263524/1‑140 (MQ=255)           tcgttcgtATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACccagcc                                                                                              >  1:103928/1‑140 (MQ=255)           tcgttcgtATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGCCGGGTTTAacccaccc                                                                                              >  2:314731/1‑140 (MQ=255)             gttcgtATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACccagccag                                                                                            <  1:31071/140‑1 (MQ=255)                    tGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGa                                                                                        >  2:388847/1‑137 (MQ=255)                    tGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGa                                                                                        <  1:388847/137‑1 (MQ=255)                           aCAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATc                                                                              >  1:270063/1‑140 (MQ=255)                           aCAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATc                                                                              <  2:103928/140‑1 (MQ=255)                            cAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATcg                                                                             <  2:263524/140‑1 (MQ=255)                            cAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATcg                                                                             <  2:258684/140‑1 (MQ=255)                                 tAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGat                                                                                       <  2:89109/125‑1 (MQ=255)                                 tAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGat                                                                                       >  1:89109/1‑125 (MQ=255)                                       gCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTaataaat                                                                  <  1:25059/140‑1 (MQ=255)                                       gCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTaataaat                                                                  <  2:270063/140‑1 (MQ=255)                                             ttCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCa                                                            <  1:203602/140‑1 (MQ=255)                                                   aaaaaCGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACt                                                      >  2:71887/1‑140 (MQ=255)                                                       aCGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTaaa                                                  >  1:394796/1‑140 (MQ=255)                                                       aCGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTaaa                                                  >  2:299287/1‑140 (MQ=255)                                                       aCGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTaaa                                                  >  1:291926/1‑140 (MQ=255)                                                            ggCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCAc                                                           >  2:291958/1‑126 (MQ=255)                                                            ggCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCAc                                                           <  1:291958/126‑1 (MQ=255)                                                                     taaGCCCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTgg                                    <  1:272068/140‑1 (MQ=255)                                                                       aGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAg                                  <  1:269101/140‑1 (MQ=255)                                                                       aGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAg                                  >  1:338273/1‑140 (MQ=255)                                                                           cTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTgg                                    >  2:131266/1‑134 (MQ=255)                                                                           cTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTgg                                    <  1:131266/134‑1 (MQ=255)                                                                           cTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGAc                              >  2:384094/1‑140 (MQ=255)                                                                                                tACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGtt         >  2:137652/1‑140 (MQ=255)                                                                                                tACAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGtt         >  2:358676/1‑140 (MQ=255)                                                                                                 aCAACTACTGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTa        <  1:137652/140‑1 (MQ=255)                                                                                                     ctactGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTa        <  2:267799/136‑1 (MQ=255)                                                                                                     ctactGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTa        >  1:267799/1‑136 (MQ=255)                                                                                                     ctactGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTgg    >  2:164599/1‑140 (MQ=255)                                                                                                       actGCATTAACAAACGCCACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTGGcc  >  1:44858/1‑140 (MQ=255)                                                                                                                        |                                                                                                                           AGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCTACGGATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTCACTGACTTAAAATAACTTTGCCTGGAGCGACAAGGATGTTAAAAAAAACGTTACTGGCC  >  NC_000913/3362589‑3362830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑