Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,775	C→T	A27T (GCA→ACA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,775	0	C	T	100.0%	49.0 / NA	15	A27T (GCA→ACA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/10);  total (5/10)

ATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTGCAAAACAAAAGTCCTACGCAGGAATTA  >  NC_000913/3469659‑3469906                                                                                                                     |                                                                                                                                    atcatTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCagag                                                                                                              <  1:41142/140‑1 (MQ=255)      ttGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGaa                                                                                                         <  1:104783/140‑1 (MQ=255)           ttCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCGCCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAacacac                                                                                                    <  1:32991/140‑1 (MQ=255)           ttCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAacacac                                                                                                    <  2:103894/140‑1 (MQ=255)           ttCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAacacac                                                                                                    <  2:193003/140‑1 (MQ=255)           ttCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAacacac                                                                                                    <  2:264247/140‑1 (MQ=255)                ttttCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGcc                                                                                               <  1:29734/140‑1 (MQ=255)                   tCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCa                                                                                            >  1:44781/1‑140 (MQ=255)                        ggCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATAc                                                                                         >  1:358349/1‑138 (MQ=255)                        ggCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATAc                                                                                         <  2:358349/138‑1 (MQ=255)                                               caGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATAtt                                                                <  2:119734/140‑1 (MQ=255)                                                      tttCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTc                                                         >  1:131428/1‑140 (MQ=255)                                                            aaaTTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATtata                                                   <  2:44781/140‑1 (MQ=255)                                                                                    gATATTTATTACCACCTTGTTGGTTATTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTGca                           >  1:56895/1‑140 (MQ=255)                                                                                                             aTTCCATGTTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTGCAAAACAAAAGTCCTACGCAGGAatta  >  1:101513/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                    ATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGCCAATGCTGGCAGAGCGGAACACACCAGCCCCATACCAATCATAGATTTAGTAAATATATTTAATTTCATTATAATCCCTTGTGACGTAAAAACTGCAAAACAAAAGTCCTACGCAGGAATTA  >  NC_000913/3469659‑3469906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑