| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,473,693 | T→C | N130S (AAC→AGC) | rpsG ← | 30S ribosomal subunit protein S7 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,473,693 | 0 | T | C | 94.1% | 54.6 / ‑4.6 | 17 | N130S (AAC→AGC) | rpsG | 30S ribosomal subunit protein S7 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (10/6); minor base G (1/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAACCGGACGGACTTCAACTGGTA > NC_000913/3473560‑3473822 | aGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTg > 2:276457/1‑140 (MQ=255) gcgcCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAgttcgtt > 2:214960/1‑140 (MQ=255) tAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGcc < 1:214960/140‑1 (MQ=255) aaaCTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCAt < 1:276457/140‑1 (MQ=255) gtgtGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACg > 1:53724/1‑135 (MQ=255) gtgtGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACg < 2:53724/135‑1 (MQ=255) cGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCa > 2:341031/1‑140 (MQ=255) gAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGGTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCaa > 2:271102/1‑140 (MQ=255) gAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCaa < 2:195385/140‑1 (MQ=255) gCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGAt > 2:61655/1‑140 (MQ=255) aaCGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTacgac > 1:307826/1‑140 (MQ=255) aaCGTCTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTacgac > 1:385588/1‑140 (MQ=255) cTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCGGAGCATTACGACGAAcc > 2:92377/1‑140 (MQ=255) cTTCACGTTTCTTAACTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAAcc > 1:216896/1‑140 (MQ=255) aaCTGCAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAACCGGACGGACTTCaa < 1:341031/140‑1 (MQ=255) cAGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAACCGGACGGACTTCAACTGGt > 2:73310/1‑140 (MQ=255) aGTACCTTTGCTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAACCGGACGGACTTCAACTGGTa < 1:61655/140‑1 (MQ=255) | AGCGGGCTGCTTACTGGAAGCGCCCGCCTGGTGACTAAAACTCCGAAGGGATAACCAACGGTAGTGTGCGAACGCCTTGTTGGCTTCGGCCATACGGTGAACGTCTTCACGTTTCTTAACTGCAGTACCTTTGTTTTCTGCAGCATCAGAAAGTTCGTTCGCCAGGCGCAGAGCCATGGATTTATCACCGCGTTTACGAGCAGCTTCAACGATCCAACGCATTGCCAGAGCATTACGACGAACCGGACGGACTTCAACTGGTA > NC_000913/3473560‑3473822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |