Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,479,075	C→T	G511G (GGG→GGA)	kefB ←	K(+) : H(+) antiporter KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,479,075	0	C	T	100.0%	70.3 / NA	21	G511G (GGG→GGA)	kefB	K(+) : H(+) antiporter KefB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

GCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAAT  >  NC_000913/3478942‑3479200                                                                                                                                      |                                                                                                                              gcaTCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgcc                                                                                                                         <  1:249734/140‑1 (MQ=255)  caTCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgcct                                                                                                                        >  2:345249/1‑140 (MQ=255)  caTCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgcct                                                                                                                        >  2:284202/1‑140 (MQ=255)   aTCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgcctg                                                                                                                       >  2:63520/1‑140 (MQ=255)        cATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgc                                                                                                                          >  2:353068/1‑132 (MQ=255)        cATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTcctgc                                                                                                                          <  1:353068/132‑1 (MQ=255)        cATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGtaata                                                                                                                  >  2:229545/1‑140 (MQ=255)             ccAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGTGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACtaa                                                                                                             >  2:138185/1‑138 (MQ=255)               aGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATg                                                                                                           <  1:345249/140‑1 (MQ=255)               aGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATg                                                                                                           <  1:284202/140‑1 (MQ=255)                     cgAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTc                                                                                                     >  1:176876/1‑140 (MQ=255)                                         ctGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTcgcg                                                                                 <  2:176876/140‑1 (MQ=255)                                                                    cGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCt                                                      >  2:170305/1‑140 (MQ=255)                                                                     gTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTg                                                     <  2:171950/140‑1 (MQ=255)                                                                             cGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCata                                             >  1:50575/1‑140 (MQ=255)                                                                                              ctAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATg                            <  1:131783/140‑1 (MQ=255)                                                                                                aaCGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGgt                          <  1:229545/140‑1 (MQ=255)                                                                                                         gagaATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCgg                 <  1:310240/140‑1 (MQ=255)                                                                                                           gaATGTTTCACGGGAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCt               <  2:50575/140‑1 (MQ=255)                                                                                                                        gAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAAt  >  1:83776/1‑140 (MQ=255)                                                                                                                        gAAAACTGCGTCACTCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAAt  <  2:339870/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                              GCATCCGCATATCCAGGCGGCGAAAATGCAGTTGCGCTCGCTGTGCCTGATGCGGATGCATGCCAAGCGTGACCAGCGTCTTGCGCCCCAGCTCTAACGCACTGGAGAATGTTTCACGGGAAAACTGCGTCACCCCTGCCTGTAATAACTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAAT  >  NC_000913/3478942‑3479200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑