Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,564,496	T→C	T696A (ACA→GCA)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,564,496	0	T	C	100.0%	68.6 / NA	20	T696A (ACA→GCA)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/14);  total (6/14)

GATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTT  >  NC_000913/3564361‑3564621                                                                                                                                        |                                                                                                                              gATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTa                                                                                                                           >  2:225148/1‑140 (MQ=255)   tAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTAcc                                                                                                                         <  2:298243/140‑1 (MQ=255)          ccGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATa                                                                                                                  <  1:211517/140‑1 (MQ=255)           cGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATaa                                                                                                                 >  1:65313/1‑140 (MQ=255)                         tGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCa                                                                                                   <  2:204058/140‑1 (MQ=255)                           aaTACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCAcc                                                                                                 >  2:6749/1‑140 (MQ=255)                                     tGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACa                                                                                             >  2:65333/1‑134 (MQ=255)                                     tGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACa                                                                                             <  1:65333/134‑1 (MQ=255)                                     tGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATcc                                                                                       <  2:179149/140‑1 (MQ=255)                                             tGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCAtctc                                                                               <  1:267988/140‑1 (MQ=255)                                                  cAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACAt                                                                          <  1:311399/140‑1 (MQ=255)                                                       ccTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCa                                                                     <  1:6749/140‑1 (MQ=255)                                                       ccTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCa                                                                     <  2:65313/140‑1 (MQ=255)                                                       ccTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCa                                                                     <  2:381416/140‑1 (MQ=255)                                                           aTGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGt                                                                 <  2:174344/140‑1 (MQ=255)                                                                      cATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCg                                                      <  1:225148/140‑1 (MQ=255)                                                                                  agtaTTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCa                                          <  1:305961/140‑1 (MQ=255)                                                                                              gTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCa                              >  1:243244/1‑140 (MQ=255)                                                                                                         tGACGACGCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGtt                   >  1:329540/1‑140 (MQ=255)                                                                                                           acgacgCAGTTCTTCCACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTAc                 >  1:264934/1‑140 (MQ=255)                                                                                                                          cACTTCTTCCGCTGCGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCtt  <  2:329540/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                              GATAGCGACCCGGATCTTCCGGACTGAATACACCGCTGCCGATTTGCGTCAGCACCTGATGCAGCTCCTCATCTTTCTCGTAGTATTCACGCGGTTTGTAGCCCTGACGACGCAGTTCTTCCACTTCTTCCGCTGTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTT  >  NC_000913/3564361‑3564621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑