Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,868	T→C	D153G (GAC→GGC)	ugpB ←	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,868	0	T	C	100.0%	48.4 / NA	15	D153G (GAC→GGC)	ugpB	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/9);  total (6/9)

CAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCG  >  NC_000913/3591732‑3591981                                                                                                                                         |                                                                                                                  cAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCtt                                                                                                                <  1:37633/140‑1 (MQ=255)   gTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTttg                                                                                                              <  2:391695/140‑1 (MQ=255)   gTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTttg                                                                                                              <  2:74906/140‑1 (MQ=255)                  gCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACg                                                                                               >  1:241271/1‑140 (MQ=255)                        cGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTc                                                                                         <  1:101202/140‑1 (MQ=255)                                  gCCGCACTTCATGCCGGAGGCTTTCAATTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCATTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGa                                                                               <  1:323396/140‑1 (MQ=255)                                                cGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGt                                                                 >  1:21164/1‑140 (MQ=255)                                                   aGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAg                                                              >  1:394887/1‑140 (MQ=255)                                                                     cATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGCGGCCCGTTTTGCTGTCg                                            <  2:394887/140‑1 (MQ=255)                                                                                    ccTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGt                                              <  1:15771/123‑1 (MQ=255)                                                                                    ccTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGaa                             >  2:205047/1‑140 (MQ=255)                                                                                    ccTGCCAGGCTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGt                                              >  2:15771/1‑123 (MQ=255)                                                                                                          ttCCGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACt       >  1:12413/1‑140 (MQ=255)                                                                                                             cGGGTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCAt    <  2:241271/140‑1 (MQ=255)                                                                                                               ggTCTAATCCTGCTTTCTTGAAGGCGCCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCg  <  2:12413/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                  CAGTTGGATCCAGCCCTGCCAGCCGCTGGCGTAGCCGCACTTCATGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCG  >  NC_000913/3591732‑3591981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑