Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,675	A→G	Y10C (TAC→TGC)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,675	0	A	G	100.0%	84.7 / NA	24	Y10C (TAC→TGC)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/14);  total (10/14)

CTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTC  >  NC_000913/427539‑427775                                                                                                                                         |                                                                                                     cTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGt                                                                                                   >  2:259704/1‑140 (MQ=255) cTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGt                                                                                                   <  2:176587/140‑1 (MQ=255)    aaTGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCAt                                                                                                <  2:110763/140‑1 (MQ=255)              cTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGGCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCgtg                                                                                      >  1:251987/1‑140 (MQ=255)              cTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCgtg                                                                                      >  2:265641/1‑140 (MQ=255)              cTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCgtg                                                                                      >  1:81984/1‑140 (MQ=255)                         attaAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCAt                                                                           >  2:255309/1‑140 (MQ=255)                          ttaAACGTGACTTCGTAGCTGCCGTCCTGGCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATc                                                                          <  2:251987/140‑1 (MQ=255)                                gTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTctg                                                                    <  1:29589/140‑1 (MQ=255)                                 tGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTctgc                                                                   >  2:7046/1‑140 (MQ=255)                                        gTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATgc                                                            <  2:136581/140‑1 (MQ=255)                                        gTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATgc                                                            <  1:284724/140‑1 (MQ=255)                                                 gTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCaa                                                   <  1:7046/140‑1 (MQ=255)                                                 gTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCaa                                                   <  1:268968/140‑1 (MQ=255)                                                       ccGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGtt                                             <  2:150201/140‑1 (MQ=255)                                                       ccGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTCTCATCGGTCTGCTGTATGCGCTTCCCAACCTGtt                                             <  2:350519/140‑1 (MQ=255)                                                           aaGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGTTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGt                                         <  1:374519/140‑1 (MQ=255)                                                                          cgcTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGtt                          <  2:81984/140‑1 (MQ=255)                                                                            cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCgg                               <  2:23784/133‑1 (MQ=255)                                                                            cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCgg                               >  1:23784/1‑133 (MQ=255)                                                                            cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCt                             >  1:357980/1‑135 (MQ=255)                                                                            cTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCt                             <  2:357980/135‑1 (MQ=255)                                                                             tGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAg                       >  1:19705/1‑140 (MQ=255)                                                                                                  aGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTGCGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTc  >  2:155398/1‑140 (MQ=255)                                                                                                                                         |                                                                                                     CTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCATCGGTCTGCTGTATGCGCTTCCCAACCTGTTTGGTGAGGATCCGGCTGTTCAGATCACTGGTGCGCGCGGAGTC  >  NC_000913/427539‑427775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑