Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,912,976	T→C	K231K (AAA→AAG)	glmS ←	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,912,976	0	T	C	100.0%	92.0 / NA	26	K231K (AAA→AAG)	glmS	L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

TCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAA  >  NC_000913/3912859‑3913104                                                                                                                      |                                                                                                                                 tCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTAt                                                                                                            >  1:387678/1‑140 (MQ=255)      tAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAg                                                                                                       <  2:387678/140‑1 (MQ=255)         gggTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATg                                                                                                    >  1:393777/1‑140 (MQ=255)            tgtTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCg                                                                                                          >  2:37380/1‑131 (MQ=255)            tgtTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCg                                                                                                          <  1:37380/131‑1 (MQ=255)                    aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGaa                                                                                                        >  2:324185/1‑125 (MQ=255)                    aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGaa                                                                                                        <  1:324185/125‑1 (MQ=255)                    aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACg                                                                                         <  2:218419/140‑1 (MQ=255)                    aTCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACg                                                                                         >  2:65263/1‑140 (MQ=255)                                            tctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAata                                                                      >  2:99752/1‑135 (MQ=255)                                            tctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAata                                                                      <  1:99752/135‑1 (MQ=255)                                            tctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 <  1:116930/140‑1 (MQ=255)                                            tctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 <  2:384898/140‑1 (MQ=255)                                                tCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 <  2:185620/136‑1 (MQ=255)                                                tCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 >  1:185620/1‑136 (MQ=255)                                                tCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCctct                                                             <  1:16031/140‑1 (MQ=255)                                                    cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 >  1:311038/1‑132 (MQ=255)                                                    cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 <  2:311038/132‑1 (MQ=255)                                                    cATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCaa                                                         >  2:380069/1‑140 (MQ=255)                                                           tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 <  1:294021/125‑1 (MQ=255)                                                           tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGcc                                                                 >  2:294021/1‑125 (MQ=255)                                                           tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                  <  1:8999/140‑1 (MQ=255)                                                                                                  ttGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa           >  1:140572/1‑140 (MQ=255)                                                                                                   tGGATTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGaa          >  1:340726/1‑140 (MQ=255)                                                                                                      aTTCGATATCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCg       >  2:262270/1‑140 (MQ=255)                                                                                                           atatCCTGACGCTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa  >  2:175118/1‑140 (MQ=255)                                                                                                                      |                                                                                                                                 TCCGGTAAGGGTGTTTTTGATCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAA  >  NC_000913/3912859‑3913104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑