| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,318,226 | A→G | V180A (GTG→GCG) | phnK ← | carbon‑phosphorus lyase subunit PhnK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,318,226 | 0 | A | G | 90.9% | 25.5 / ‑5.7 | 12 | V180A (GTG→GCG) | phnK | carbon‑phosphorus lyase subunit PhnK |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (5/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCACCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAAGGTGGTCGGCAGGTCGTCGATCCG > NC_000913/4318098‑4318353 | gCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATcc > 2:237424/1‑140 (MQ=255)gCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATcc > 2:28800/1‑140 (MQ=255) cAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGtt > 1:24304/1‑140 (MQ=255) gCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCa < 2:24304/140‑1 (MQ=255) gcagGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGc < 2:51840/140‑1 (MQ=255) ggcgACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGAt < 1:237424/140‑1 (MQ=255) gggTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCgg < 2:356016/140‑1 (MQ=255) ccaccGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCaaa > 1:144513/1‑140 (MQ=255) ccaccaGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCCCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCgg < 2:290457/138‑1 (MQ=255) ccaccaGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCACCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCgg > 1:290457/1‑138 (MQ=255) caccaGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGaaa < 1:28800/140‑1 (MQ=255) aGGCGGGCCTGCGCCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAAGGTGGTCGGCAGGTCGTCGATCCg > 1:31594/1‑140 (MQ=255) | GCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCCTGCACCGACACATCCAGCCCGCCGGTCGGTTCATCCATAAACACCAGCTTCGGATGCGTCACCAGGTTGCGGGCAATCTGCAAACGCTGCTGCATACCGCCGGAAAAGGTGGTCGGCAGGTCGTCGATCCG > NC_000913/4318098‑4318353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |