Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,444,104	C→T	P575S (CCA→TCA)	tamA →	translocation and assembly module subunit TamA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,444,104	0	C	T	100.0%	97.5 / NA	30	P575S (CCA→TCA)	tamA	translocation and assembly module subunit TamA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

TTCGCCGCAGCGACTTTAAAACCGGTACCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCG  >  NC_000913/4443967‑4444233                                                                                                                                          |                                                                                                                                  ttCGCCGCAGCGACTTTAAAACCGGTACCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCa                                                                                                                                 >  1:36736/1‑140 (MQ=255)                  aaaaCCGGTACCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATgg                                                                                                                >  1:18197/1‑140 (MQ=255)                        ggTACCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGaaaaaa                                                                                                          >  1:294211/1‑140 (MQ=255)                           aCCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATc                                                                                                       >  1:13384/1‑140 (MQ=255)                                ggTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCt                                                                                                  <  2:18197/140‑1 (MQ=255)                                        tGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTgg                                                                                          <  2:116748/140‑1 (MQ=255)                                        tGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTgg                                                                                          <  2:294211/140‑1 (MQ=255)                                            cTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGgttat                                                                                      <  2:13384/140‑1 (MQ=255)                                                                       aCTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGt                                                           <  2:218896/140‑1 (MQ=255)                                                                        cTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTAc                                                                           >  2:350228/1‑123 (MQ=255)                                                                        cTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTAc                                                                           <  1:350228/123‑1 (MQ=255)                                                                                          gTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGcacc                                        >  2:237859/1‑140 (MQ=255)                                                                                              cgGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCAccacca                                    <  1:98544/140‑1 (MQ=255)                                                                                                gATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGc                                  >  1:324527/1‑140 (MQ=255)                                                                                                     aGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCt                             >  1:265628/1‑140 (MQ=255)                                                                                                      gACGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTg                            <  1:163184/140‑1 (MQ=255)                                                                                                       aCGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGc                           <  1:73123/140‑1 (MQ=255)                                                                                                       aCGAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGc                           <  1:391290/140‑1 (MQ=255)                                                                                                         gAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCAt                         <  2:265628/140‑1 (MQ=255)                                                                                                         gAACACGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTCTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCAt                         <  1:332699/140‑1 (MQ=255)                                                                                                            cacGGGTTACAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTg                      <  2:34341/140‑1 (MQ=255)                                                                                                                     cAGTTTTACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTaaa             >  1:201783/1‑140 (MQ=255)                                                                                                                        ttttACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcg          <  1:237859/140‑1 (MQ=255)                                                                                                                          ttACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcggc        >  2:140579/1‑140 (MQ=255)                                                                                                                           tACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcggcg       >  1:128506/1‑140 (MQ=255)                                                                                                                           tACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcggcg       >  2:185707/1‑140 (MQ=255)                                                                                                                           tACATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAgcggcg       >  2:357835/1‑140 (MQ=255)                                                                                                                            aCATCGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGcggcgg      <  2:324527/140‑1 (MQ=255)                                                                                                                                cGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCg  >  1:259894/1‑140 (MQ=255)                                                                                                                                cGGTCTGGGGTCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCg  >  1:162603/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  TTCGCCGCAGCGACTTTAAAACCGGTACCGGGGTCGGCGTGCGCTGGGAATCGCCGGTCGGGCCAATCAAACTCGATTTTGCCGTACCGGTCGCGGATAAAGACGAACACGGGTTACAGTTTTACATCGGTCTGGGGCCAGAATTATGAGTTTATGGAAAAAAATCAGCCTCGGCGTGGTTATCGTTATCTTACTGTTGCTGGGATCGGTGGCGTTTCTGGTGGGCACCACCAGCGGCCTGCATCTGGTATTTAAAGCGGCGGATCG  >  NC_000913/4443967‑4444233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑