Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,594,568	A→G	V52A (GTA→GCA)	lgoT ←	galactonate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,594,568	0	A	G	100.0%	57.1 / NA	17	V52A (GTA→GCA)	lgoT	galactonate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/12);  total (5/12)

AGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATG  >  NC_000913/4594439‑4594702                                                                                                                                  |                                                                                                                                        aGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGccgccg                                                                                                                                   <  2:65756/136‑1 (MQ=255) aGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGccgccg                                                                                                                                   >  1:65756/1‑136 (MQ=255)               gTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTTACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAacaaca                                                                                                                <  1:260448/140‑1 (MQ=255)               gTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAacaaca                                                                                                                <  1:181161/140‑1 (MQ=255)                 aaGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATg                                                                                                              >  1:210022/1‑140 (MQ=255)                     gAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCgg                                                                                                          <  2:264225/140‑1 (MQ=255)                      aGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCggt                                                                                                         >  1:104106/1‑140 (MQ=255)                                   agagCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGt                                                                                            <  2:210022/140‑1 (MQ=255)                                     agCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGttt                                                                                          <  2:104106/140‑1 (MQ=255)                                        aaaGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAAt                                                                                       <  2:74951/140‑1 (MQ=255)                                                 aTTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACt                                                                              >  1:266948/1‑140 (MQ=255)                                                         ggCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGCAAAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGa                                                                       <  2:330245/140‑1 (MQ=255)                                                                        ttcttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCtggtg                                                       >  2:346871/1‑140 (MQ=255)                                                                           ttcACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCa                                                    <  1:346871/140‑1 (MQ=255)                                                                                  aTCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAat                                             <  1:324208/140‑1 (MQ=255)                                                                                                                   tCGAGATAATTGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAAcg            <  1:121635/140‑1 (MQ=255)                                                                                                                             tGATTGCCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATg  <  1:104739/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        AGTTGCGCAATCCCGTAAGCGAGTGAAAACACGGAGAGCAAAGCGCCGATTTCGGTGGCACTTAATCCCAATTCTTCACGAATCGTTAAATTTGCTACCGACAGCGAACTGCGGTCGAGATAATTGATTACCGCCGC‑AAAAAATAATAACAACATGGCGGTGGTTTGAATGCGTTTAATTCGGGTACTGCGTTGAACTAATCCATCTGGTGGCACGGGAATATCTGCCGACGAAGATGGAGTAAATGAAGAACGCGGATCGATG  >  NC_000913/4594439‑4594702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑