Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	637,533	T→C	K29R (AAA→AGA)	ybdO ←	putative LysR family DNA‑binding transcriptional regulator YbdO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	637,533	0	T	C	100.0%	72.9 / NA	21	K29R (AAA→AGA)	ybdO	putative LysR family DNA‑binding transcriptional regulator YbdO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/6);  total (15/6)

ACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAA  >  NC_000913/637408‑637662                                                                                                                              |                                                                                                                                  aCCCTGTTGTCGTCGGCGCGATACCTTTTCCTTAGCTAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCTTTGTAACGACTGGTTCACGGCTGATGGGGTGATATATAGTGAGTCAGCAGCTCTGCTAAGGCTTaaa                                                                                                                     <  2:346119/140‑1 (MQ=255)      gttgtCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTg                                                                                                                >  1:207480/1‑140 (MQ=255)            gtcgGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAAt                                                                                                          >  1:29217/1‑140 (MQ=255)                                  aGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACt                                                                                                     >  2:67531/1‑123 (MQ=255)                                  aGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACt                                                                                                     <  1:67531/123‑1 (MQ=255)                                                 ggTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACttt                                                                     >  1:219886/1‑140 (MQ=255)                                                 ggTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACttt                                                                     >  2:149322/1‑140 (MQ=255)                                                    cATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTc                                                                  >  1:70568/1‑140 (MQ=255)                                                          aTTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCg                                                            <  2:197388/140‑1 (MQ=255)                                                              cgcACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTaga                                                        >  2:301234/1‑140 (MQ=255)                                                                                   cTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCg                                   >  1:197137/1‑140 (MQ=255)                                                                                   cTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCg                                   >  1:344788/1‑140 (MQ=255)                                                                                              cTGATGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATAttttt                        <  2:70568/140‑1 (MQ=255)                                                                                                 aTGGGGTGATATATAGCGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTttctt                     >  2:170979/1‑140 (MQ=255)                                                                                                  tGGGGTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTttcttc                    <  1:301234/140‑1 (MQ=255)                                                                                                   ggggTGATATATAGTGATTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTcc                   <  2:207480/140‑1 (MQ=255)                                                                                                                   aTTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTa   >  2:105147/1‑140 (MQ=255)                                                                                                                   aTTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTa   >  1:75460/1‑140 (MQ=255)                                                                                                                   aTTCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTa   >  1:114188/1‑140 (MQ=255)                                                                                                                    ttCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATg                <  2:312304/126‑1 (MQ=255)                                                                                                                    ttCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATg                >  1:312304/1‑126 (MQ=255)                                                                                                                    ttCAGCAGCTCTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTaa  >  2:36722/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                  ACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAA  >  NC_000913/637408‑637662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑