Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	653,178	C→T	A315V (GCC→GTC)	dpiB →	sensory histidine kinase DpiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	653,178	0	C	T	100.0%	59.4 / NA	18	A315V (GCC→GTC)	dpiB	sensory histidine kinase DpiB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

AAGTGGTCAGGCCCGCCGATTTCTTTACCGAACAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGCCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCAA  >  NC_000913/653046‑653304                                                                                                                                     |                                                                                                                               aaGTGGTCAGGCCCGCCGATTTCTTTACCGAACAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATc                                                                                                                         >  1:37406/1‑140 (MQ=255)      gTCAGGCCCGCCGATTTCTTTACCGAACAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCtt                                                                                                                    >  2:241568/1‑140 (MQ=255)                                 cAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCt                                                                                         <  2:177144/140‑1 (MQ=255)                                         tgaAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCAcc                                                                                           >  2:64229/1‑130 (MQ=255)                                         tgaAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCAcc                                                                                           <  1:64229/130‑1 (MQ=255)                                                     ggATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCaaa                                                                     <  1:329000/140‑1 (MQ=255)                                                      gATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAAt                                                                    >  2:231001/1‑140 (MQ=255)                                                                      ttAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTgag                                                    <  1:241568/140‑1 (MQ=255)                                                                                              aCCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACTCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAg                            <  2:75713/140‑1 (MQ=255)                                                                                                 gggAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCAt                         <  1:38523/140‑1 (MQ=255)                                                                                                  ggAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCAtc                        >  1:300257/1‑140 (MQ=255)                                                                                                   gAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCAtct                       >  1:290117/1‑140 (MQ=255)                                                                                                     aGCTATTCGTTCAGGTGATGATTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCa                     >  2:62464/1‑140 (MQ=255)                                                                                                             gTTCAGGTGATGATTTGCT‑GGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGt            <  2:196422/140‑1 (MQ=255)                                                                                                                      atgatTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTc    >  1:254250/1‑140 (MQ=255)                                                                                                                       tgatTTGCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCa   <  2:254250/140‑1 (MQ=255)                                                                                                                             gCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCaa  >  2:254486/1‑135 (MQ=255)                                                                                                                             gCTGGGGGTCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCaa  <  1:254486/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                               AAGTGGTCAGGCCCGCCGATTTCTTTACCGAACAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATTGCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGCCATTATCAGCTTTCGTAGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAGAGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCAA  >  NC_000913/653046‑653304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑