Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,766	A→G	V673A (GTC→GCC)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,766	0	A	G	100.0%	79.9 / NA	23	V673A (GTC→GCC)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/10);  total (13/10)

TCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGT  >  NC_000913/672643‑672898                                                                                                                            |                                                                                                                                     tcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCg                                                                                                                      >  2:5562/1‑140 (MQ=255) tcGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCg                                                                                                                      >  2:215053/1‑140 (MQ=255)     tCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTa                                                                                                                  <  2:12992/140‑1 (MQ=255)       aCTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGc                                                                                                                <  2:7013/140‑1 (MQ=255)                   gTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACAcc                                                                                                    >  1:244672/1‑140 (MQ=255)                          gCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTcc                                                                                             <  1:215053/140‑1 (MQ=255)                              aTCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGcc                                                                                         >  1:14856/1‑140 (MQ=255)                                      gCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCgaga                                                                                 <  1:238627/140‑1 (MQ=255)                                          cgcTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCa                                                                             >  2:23627/1‑140 (MQ=255)                                                         agtcagCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAg                                                              >  2:281933/1‑140 (MQ=255)                                                            cagCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCaa                                                           <  2:14856/140‑1 (MQ=255)                                                            cagCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCaa                                                           <  2:300150/140‑1 (MQ=255)                                                             agCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCaaa                                                          <  2:244672/140‑1 (MQ=255)                                                               cgcATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAAca                                                        >  1:4289/1‑140 (MQ=255)                                                                               gCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAAc                                        <  1:57131/140‑1 (MQ=255)                                                                                 cGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACgg                                      >  1:37393/1‑140 (MQ=255)                                                                                 cGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACgg                                      >  1:8533/1‑140 (MQ=255)                                                                                                     tgtgCTCGTAAACCAGTTTCCGGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCa                  >  1:369758/1‑140 (MQ=255)                                                                                                      gtgCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCaa                 >  2:113064/1‑140 (MQ=255)                                                                                                      gtgCTCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCaa                 >  1:227074/1‑140 (MQ=255)                                                                                                          tCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCAt             <  1:281933/140‑1 (MQ=255)                                                                                                          tCGTAAACCAGTTTCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCAt             <  1:113064/140‑1 (MQ=255)                                                                                                                     tttCCAGGCACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGt  >  1:268226/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                     TCGGTCACTTTAGCGATCGTTTTATGCACATCGCGACGCAGCGCTTTCTGATTTTCAGTCAGCGCATCAACGTTCAGTGCCGCAACATCACCTTTTGCTGTGTGCTCGTAAACCAGTTTCCAGACACGTTTCAGGAAGCGGTTAGCCCCTTCCACACCGGATTCCTGCCATTCGAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGT  >  NC_000913/672643‑672898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑